In Vitro Heavy Metals Inhibitive Assay Using the Acetylcholinesterase from Osteochilus hasselti (Cyprinid Fish)

Authors

  • Mohd Khalizan Sabullah Faculty of Science and Natural Resources, Universiti Malaysia Sabah, 88400 Kota Kinabalu, Sabah, Malaysia.
  • Kavilasni Subramaniam Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Ain Aqilah Basirun Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Abubakar M. Umar Department of Biological Sciences, Faculty of Science, Gombe State University, P.M.B. 027, Gombe, Nigeria.

DOI:

https://doi.org/10.54987/jobimb.v7i2.480

Keywords:

Osteochilus hasselti; acetylcholinesterase; heavy metals; in vitro assay; biomonitoring

Abstract

Rapid industrial development has caused many hazardous toxicants, especially heavy metals, to be released directly or indirectly into the environment which resulted in the polluted environment, mainly the water bodies. There are numerous Malaysian rivers that are largely polluted by heavy metals which can cause negative impact on health of public as well as the environment. Hence, a rapid and simple biomonitoring method will aid in notifying government agencies as well the public to such threat. Accordingly, acetylcholinesterase (AChE) inhibition has been widely used as a biomarker for heavy metals detection. In this study, the potential of AChE from Osteochilus hasselti brain as an alternative biosensor for heavy metals detection is measured. We discovered that out of seven heavy metals tested at the final concentration of 5 mg/L, only Hg2+, Ag2+, and Cu2+ exerted more than 50% significant inhibition (p<0.05) based on ANOVA analysis. The As5+ and Cd2+ exhibited inhibition by lowering the activity of AChE to less than 50%, displaying no significant difference (ð‘ƒð‘ƒ > 0.05) compared to each other. Meanwhile, the Cr6+ and Pb2+ showed no notable inhibitory effect on the activity of AChE. The results exhibited that AChE of O. hasselti has the capability to be used as a biosensor for the detection of metal ions.

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Published

26.12.2019

How to Cite

Sabullah, M. K., Subramaniam, K., Basirun, A. A., & Umar, A. M. (2019). In Vitro Heavy Metals Inhibitive Assay Using the Acetylcholinesterase from Osteochilus hasselti (Cyprinid Fish). Journal of Biochemistry, Microbiology and Biotechnology, 7(2), 30–34. https://doi.org/10.54987/jobimb.v7i2.480

Issue

Vol. 7 No. 2 (2019)

Section

Articles

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