Probiotic Properties of Lactobacillus Isolates from Chicken Intestines

Authors

  • Wan Nur Fadhilah Shamsudin institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor.
  • Shu San Loo Agro-Biotechnology Institute Malaysia (ABI), c/o MARDI Headquarters, 43400 Serdang, Selangor.
  • Yin Wan Ho Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor.
  • Norhani Abdullah institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor.
  • Wan Zuhainis Saad Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Kiew Lian Wan School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia.

DOI:

https://doi.org/10.54987/jobimb.v7i2.476

Keywords:

Lactobacillus; probiotic properties; antagonistic effects; pathogens

Abstract

Three lactic acid bacteria (LAB) isolates which demonstrated probiotic properties were obtained from chicken intestine. The isolates demonstrated good tolerance to acidic pH 3, 0.3% bile salts and strong adhesion to Caco-2 cells. These isolates also showed antagonistic activity against pathogenic E. coli (078:K80), Salmonella enterica serovar Typhimurium, C. perfringens (Type A), Salmonella enterica serovar Enteritidis, E. faecium (FM3), E. faecalis (E227). All three isolates had high inhibition zone (> 6 mm) against E. coli 078:K80 and Salmonella enterica serovar Typhimurium and were susceptible to antibiotics gentamicin, kanamycin, streptomycin, tetracycline, erythromycin, clindamycin, chloramphenicol and ampicillin. These isolates were identified by using 16S rRNA sequencing technique and as two L. salivarius and one L. reuteri with accession number MH375403, MH375402 and MH375404, respectively.

References

Tierney, J., Gowing, H., Van Sinderen, D., Flynn, S., Stanley, L., McHardy, N., and Mulcahy, G. In vitro inhibition of Eimeria tenella invasion by indigenous chicken Lactobacillus species. Vet Parasitol. 2004. 122(3): 171–182.

FAO/WHO (Food and Agriculture Organization/World Health Organization). Health and Nutritional Properties of Probiotics in Food including Powder Milk with Live Lactic Acid Bacteria.Report of a Joint FAO/WHO expert consultation on evaluation of healt and nutritional properties of probiotics in food including powder milk with lactic acid bacteria. 2001. 1-34.

Markowiak, P., and Śliżewska, K. The role of probiotics, prebiotics and synbiotics in animal nutrition. Gut Pathog. 2018. 10(1): 21.

Ouled-Haddar, H., Idoui, T., Sifour, M., Guezira, M., and Bouthabet, M. Isolation, characterization and microencapsulation of probiotic Lactobacillus curvatus G7 from chicken crop. Online J Sci Technol. 2012. 2(1)1-6.

Heravi, R.,M., Kermanshahi, H., Sankian,M., Nassiri,M.,R., Moussavi.,A.,H., Nsiraii,L.,R., Varasteh.,A.,R. Screening of lactobacilli bacteria isolated from gastrointestinal tract of broiler chickens for their use as probiotic. Afr J Microbiol Res. 2011. 5(14): 1858-1868.

Black, J.G. Microbiol. John Wiley and Sons (Asia) Pte. Ltd. Seventh Edition. 2008.

Ehrmann, M. a., Kurzak, P., Bauer, J., and Vogel, R. F. Characterization of lactobacilli towards their use as probiotic adjuncts in poultry. J Appl Microbiol. 2002. 92(5): 966–975.

Jin, L. Z., Ho, Y. W., Abdullah, N., and Jalaludin, S. Acid and bile tolerance of Lactobacillus isolated from chicken intestine. Lett Appl Microbiol. 1998. 27(3): 183–185.

Duary, R. K., Rajput, Y. S., Batish, V. K., and Grover, S. Assessing the adhesion of putative indigenous probiotic lactobacilli to human colonic epithelial cells. Indian J Med Res. 2011.134(11): 664–671.

El-Kholy, A. M., El-Shinawy, S. H., Meshref, A. M. S. M. S., and Korny, A. M. M. Screening of antagonistic activity of probiotic bacteria against some food-borne pathogens. J Appl and Environmental Microbiol. 2014. 2(2): 53–60.

Carasi, P., Díaz, M., Racedo, S. M., Antoni, G. De, Urdaci, M. C., and Serradell, M. de los A. Safety characterization and antimicrobial properties of kefir-isolated Lactobacillus kefiri. BioMed Res Int. 2014: 1–7.

Abbas Hilmi, H. T., Surakka, A., Apajalahti, J., and Saris, P. E. J. Identification of the most abundant Lactobacillus species in the crop of 1- and 5-week-old broiler chickens. Appl Environ Microbiol. 2007. 73(24): 7867–7873.

Saitou, N., and Nei, S. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987.4(4):406-425.

Bujnakova, D., Strakova, E., and Kmet, V. In vitro evaluation of the safety and probiotic properties of Lactobacilli isolated from chicken and calves. Anaerobe. 2014. 29: 118–127.

Sanhueza, E., Paredes-osses, E., González, C. L., and García, A. Electronic J bioTechnol effect of pH in the survival of Lactobacillus salivarius strain UCO _ 979C wild type and the pH acid acclimated variant. Electron J BioTechnol. 2015. 18(5): 343–346.

Cotter, P. D., and Hill, C. Antimicrobial activity - The most important property of probiotic and starter lactic acid bacteria. Food Technol BioTechnol. 2003. 67(3): 429–453.

Wang, Z., Wang, L., Chen, Z., Ma, X., Yang, X., Zhang, J., and Jiang, Z. In vitro evaluation of swine-derived Lactobacillus reuteri: Probiotic properties and effects on intestinal porcine epithelial cells challenged with enterotoxigenic Escherichia coli K88. J Microbiol BioTechnol. 2016. 26(6): 1018–1025.

Pfeiler, E. A., and Klaenhammer, T. R. Role of transporter proteins in bile tolerance of Lactobacillus acidophilus. Appl Environ Microbiol. 2009. 75(18): 6013–6016.

Lambert, J. M., Bongers, R. S., De Vos, W. M., and Kleerebezem, M. Functional analysis of four bile salt hydrolase and penicillin acylase family members in Lactobacillus plantarum WCFS1. Appl Environ Microbiol. 2008. 74(15): 4719–4726.

Ruiz, L., Margolles, A., and Sánchez, B. Bile resistance mechanisms in Lactobacillus and Bifidobacterium. Front Microbiol 4 (2013): 396

Fontana, L., Bermudez-Brito, M., Plaza-Diaz, J., And, S. M.-Q., and Gil, A. Sources , isolation , characterisation and evaluation of probiotics. Br J Nutr. 2013. 109: S35–S50.

Zommiti, M., Connil, N., Hamida, J. Ben, and Ferchichi, M. Probiotic Characteristics of Lactobacillus curvatus DN317, a Strain Isolated from Chicken Ceca. Probiotics Antimicrob Protein. 2017. 9(4): 415–424.

Shokryazdan, P., Sieo, C. C., Kalavathy, R., Liang, J. B., Alitheen, N. B., Faseleh Jahromi, M., and Ho, Y. W. Probiotic potential of Lactobacillus strains with antimicrobial activity against some human pathogenic strains. BioMed Res International. 2014: 1-16.

Thakur, N., Angad, G., Veterinary, D., Rokana, N., Panwar, H., Angad, G., and Veterinary, D. Probiotics: Selection criteria, safety and role in health and disease, J Innovative Biol. 2016. 3(1): 259-270.

Andrabi, S. T., Bhat, B., Gupta, M., and Bajaj, B. K. Phytase-producing potential and other functional attributes of lactic acid bacteria isolates for prospective probiotic applications. Probiotics Antimicrob Protein. 2016. 8(3): 121–129.

Aka-gbezo, S., Konan, A. G., Cho, M. N., Achi, P., Koffi-nevry, R., Koussemon-camara, M., and Bonfoh, B. Screening of antimicrobial activity of lactic acid bacteria isolated from anango baca slurry, a spontaneously fermented maize product used in Côte d’Ivoire. Int J Biol Chem Sci. 2017. 11(6): 2616–2629.

Dec, M., Puchalski, A., Urban-Chmiel, R., and Wernicki, A. Screening of Lactobacillus strains of domestic goose origin against bacterial poultry pathogens for use as probiotics. Poultry Sci. 2014. 93(10): 2464–2472.

Manini, F, M.,C., Casiraghi, K., Poutanen, M., Brasca, D., Erba, C., Plumed-Ferrer. Characterization of lactic acid bacteria isolated from wheat bran sourdough. Food Sci Technol. 2015. 66: 275 – 283.

Chandok, H., Shah, P., Akare, U. R., Hindala, M., Bhadoriya, S. S., Ravi, G. V., and Nayarisseri, A. Screening, isolation and identification of probiotic producing Lactobacillus acidophilus Strains EMBS081 and EMBS082 by 16S rRNA Gene Sequencing. Interdisciplinary Scis: Comp Life Sci. 2014.7(3): 242–248.

Rajoka, M. S. R., Hayat, H. F., Sarwar, S., Mehwish, H. M., Ahmad, F., Hussain, N.,Shi, J. Isolation and evaluation of probiotic potential of lactic acid bacteria isolated from poultry intestine. Microbiol (Russian Federation). 2018. 87(1): 116–126.

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Published

26.12.2019

How to Cite

Shamsudin, W. N. F., Loo, S. S., Ho, Y. W., Abdullah, N., Saad, W. Z., & Wan, K. L. (2019). Probiotic Properties of Lactobacillus Isolates from Chicken Intestines. Journal of Biochemistry, Microbiology and Biotechnology, 7(2), 8–13. https://doi.org/10.54987/jobimb.v7i2.476

Issue

Vol. 7 No. 2 (2019)

Section

Articles

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