Probiotics and Postbiotics from Southeast Asian Traditional Fermented Foods Highlight Functional Potential in Malaysia

Authors

  • Sakina Shahabudin Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang Al-Sultan Abdullah
  • Mohd Faizulnazrie Masri Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300 Kuantan, Pahang, Malaysia.
  • Nina Suhaity Azmi Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300 Kuantan, Pahang, Malaysia.

DOI:

https://doi.org/10.54987/jobimb.v13i2.1157

Keywords:

Fermented foods, Probiotics, Postbiotics, Malaysia, Lactic acid bacteria

Abstract

Fermented foods are essential in Southeast Asia's culinary and cultural landscapes, providing nutrition, preservation, and microbial diversity. Beyond their traditional significance, these foods include beneficial probiotics and metabolic by-products, known as postbiotics, that improve food functionality and human health. This review compiles recent research on the probiotic diversity and postbiotic potential of fermented foods from Southeast Asia, with a focus on traditional Malaysian variations such as budu, tempoyak, belacan, cincalok, tapai, pekasam, and pickled maman. It covers the most common microbial species, fermentation substrates, and bioactive metabolites in the fermented foods, while also highlighting recently discovered postbiotic components such as exopolysaccharides, peptides, and short-chain fatty acids. Despite these advantages, Malaysian traditional fermented foods have received minimal research, particularly in metabolomic characterization and clinical validation. Omics tools integration, safety assessment, and the valuation of local probiotic strains for functional food innovation will be beneficial in the next steps for fermentation science in Malaysia.

References

Tharmabalan S, Rusli SA, Lo R, Saidin NFB, Basar Z. From tradition to Table: an introduction to the culture and nutritional significance of Malaysian fermented foods products. J Ethn Foods. 2025;12(1):45–62. https://doi.org/10.1186/s42779-025-00278-2

Food and Agriculture Organization of the United Nations, World Health Organization. Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Geneva: World Health Organization; 2001.

Salminen S, Collado MC, Endo A, Hill C, Lebeer S, Quigley EMM, et al. The International Scientific Association of Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of postbiotics. Nat Rev Gastroenterol Hepatol. 2021;18:649–667.

Anggadhania L, Setiarto RHB, Yusuf D, Anshory L, Royyani MF. Exploring tempoyak, fermented durian paste, a traditional Indonesian indigenous fermented food: typical of Malay tribe. J Ethn Foods. 2023;10:42. https://doi.org/10.1186/s42779-023-00206-2

Md Zoqratt MZH, Gan HM. The inconsistent microbiota of Budu, the Malaysian fermented anchovy sauce, revealed through 16S amplicon sequencing. PeerJ. 2021;9:e12345. https://doi.org/10.7717/peerj.12345

Hussin M, Anzian A, Liew CXQ, Muhialdin BJ, Mohsin AZ, Fang CM, et al. Potentially probiotic fermented glutinous rice (Oryza sativa L.) with Lactiplantibacillus plantarum improved immune system response in BALB/cByJ mice. Fermentation. 2022;8(11):612. https://doi.org/10.3390/fermentation8110612

Terpou A, Dahiya D, Nigam PS. Evolving dynamics of fermented food microbiota and the gut microenvironment: strategic pathways to enhance human health. Foods. 2025;14(13):2361. https://doi.org/10.3390/foods14132361

Xing Y, Huang M, Olovo CV, Mgbechidinma CL, Yang Y, Liu J, et al. Traditional fermented foods: challenges, sources, and health benefits of fatty acids. Fermentation. 2023;9(2):110. https://doi.org/10.3390/fermentation9020110

Iram P, Samuel K, Tonga NG, Ajibola OO, Kelvin E. The Bidayuh ethnic’s fermented foods: a review as potential sources of probiotics. Int Postgrad Semin Agric For. 2025;8(2):88–105.

Mohd Kamalul Abrar NH, Md Jaffri J. The health benefits of fermented food: a narrative review. Malays J Sci. 2023;42(1):78–91. https://doi.org/10.22452/mjs.vol42no1.8

Tamang JP, Watanabe K, Holzapfel WH. Diversity of microorganisms in global fermented foods and beverages. Front Microbiol. 2016;7:377. https://doi.org/10.3389/fmicb.2016.00377

Narzary Y, Das S, Goyal AK, Lam SS, Sarma H, Sharma D. Fermented fish products in South and Southeast Asian cuisine: indigenous technology, nutrient composition, and cultural significance. J Ethn Foods. 2021;8(1):33. https://doi.org/10.1186/s42779-021-00109-0

Cha YJ, Yu D. Health benefits and functions of salt-fermented fish. J Ethn Foods. 2024;11:34. https://doi.org/10.1186/s42779-024-00251-5

Lynch KM, Zannini E, Wilkinson S, Daenen L, Arendt EK. Physiology of acetic acid bacteria and their role in vinegar and fermented beverages. Compr Rev Food Sci Food Saf. 2019;18(3):587–625. https://doi.org/10.1111/1541-4337.12440

Yusmarini, Johan VS, Fitriani S, Rahmayuni, Artanti VF, Pato U. Characteristics of probiotic tapai made by addition of Lactobacillus plantarum 1. Int J Agric Technol. 2019;15(1):195–206.

Sampsell K, Marcolla CS, Tapping S, Fan Y, Sánchez-Lafuente CL, Willing BP, et al. Current research in fermented foods: bridging tradition and science. Adv Nutr. 2025;16(12):100554. https://doi.org/10.1016/j.advnut.2025.100554

Shah AM, Tarfeen N, Mohamed H, Song Y. Fermented foods: their health-promoting components and potential effects on gut microbiota. Fermentation. 2023;9(2):118. https://doi.org/10.3390/fermentation9020118

Tamang JP, Cotter PD, Endo A, Han NS, Kort R, Liu SQ, et al. Fermented foods in a global age: East meets West. Compr Rev Food Sci Food Saf. 2020;19:1–34. https://doi.org/10.1111/1541-4337.12520

Pauzi RY, Astuti DI. Exploring microbial succession in Indonesian fermented soybean paste (tauco) through a culture-dependent approach. Microbiol Biotechnol Lett. 2025;53:57–67. https://doi.org/10.48022/mbl.2411.11002

Amatachaya A, Siramolpiwat S, Kraisorn M, Yasiri A. Gamma-aminobutyric acid producing Lactiplantibacillus pentosus isolated from fermented spider plant. J Pure Appl Microbiol. 2023;17(1):354–361. https://doi.org/10.22207/JPAM.17.1.25

Okfrianti Y, Herison C, Fahrurrozi, Budiyanto. Identification of lactic acid bacteria isolated from ethnic fermented bamboo shoot Lemea. Food Res. 2023;7(Suppl 1):145–150. https://doi.org/10.26656/fr.2017.7(S1).29

Teoh SQ, Chin NL, Chong CW, Mat Ripen A, How S, Lim JJL. Health benefits and processing of tempeh with outlines on its functional microbes. Future Foods. 2024;9:100330. https://doi.org/10.1016/j.fufo.2024.100330

Hernández-Gómez JG, López-Bonilla A, Trejo-Tapia G, Ávila-Reyes SV, Jiménez-Aparicio AR, Hernández-Sánchez H. In vitro bile salt hydrolase activity screening of probiotic microorganisms. Foods. 2021;10(3):674. https://doi.org/10.3390/foods10030674

Lestari SD, Hussain N, Meor Hussin AS, Mustafa S, Sew YS. Bacillus multi-strain from Malaysian fish sauces demonstrating enzymatic and glutamic acid production activities. Appl Food Biotechnol. 2024;11(1):e26.

Calvanese CM, Villani F, Ercolini D, De Filippis F. Postbiotics versus probiotics: possible new allies for human health. Food Res Int. 2025;217:116869. https://doi.org/10.1016/j.foodres.2025.116869

Hamdi A, Lloyd C, Eri R, Van TTH. Postbiotics: a promising approach to combat age-related diseases. Life (Basel). 2025;15(8):1190. https://doi.org/10.3390/life15081190

Eraghieh Farahani H, Pourhajibagher M, Asgharzadeh S, Bahador A. Postbiotics: novel modulators of gut health and metabolism. Probiotics Antimicrob Proteins. 2025. https://doi.org/10.1007/s12602-025-10832-8

Khalil ES, Abd Manap MY, Mustafa S, Alhelli AM, Shokryazdan P. Probiotic properties of exopolysaccharide-producing Lactobacillus strains from tempoyak. Molecules. 2018;23(2):398. https://doi.org/10.3390/molecules23020398

Peres Fabbri L, Cavallero A, Vidotto F, Gabriele M. Bioactive peptides from fermented foods. Foods. 2024;13(21):3369. https://doi.org/10.3390/foods13213369

Shahabudin S, Masri MF, Lani MN, Azmi NS. Antifungal activity of lactic acid bacteria from Malaysian fermented foods. Malays J Biochem Mol Biol. 2022;1:8–15.

Abdul Rahman S, Abdul Kahar A, Mansor A, Murni DL, Hussin A, Sharifudin SA, et al. Identification of indigenous microbes with antimicrobial activity from fermented vegetables. Sci Heritage J. 2017;1(1):01–03. http://doi.org/10.26480/gws.01.2017.01.03

Irakoze ML, Wafula EN, Owaga EE. Effect of lactic acid fermentation on phytochemicals and antioxidant capacity of African vegetables. Bacteria. 2023;2(1):48–59. https://doi.org/10.3390/bacteria2010004

Ajibola OO, Thomas R, Bakare BF. Fermented indigenous vegetables and fruits from Malaysia as probiotics sources. Food Sci Hum Wellness. 2023;12:1493–1509. http://doi.org/10.1016/j.fshw.2023.02.011

Chhikara N, Devi HR, Jaglan S, Sharma P, Gupta P, Panghal A. Bioactive compounds and health benefits of Parkia speciosa. Agric Food Secur. 2018;7:45. https://doi.org/10.1186/s40066-018-0197-x

Li H, Chen C, Li Y, Li Z, Li C, Luan C. Antioxidant effects and probiotic properties of Latilactobacillus sakei. Foods. 2023;12(23):4276. https://doi.org/10.3390/foods12234276

Khadang Nikfarjam A, Mahdian E, Ahmadzadeh Ghavidel R, Karazhyan R. Probiotic potential of Lactobacillus strains from pickled garlic. J BioSci Biotechnol. 2021;10(1):41–51.

Xu J, Zeng Z, Zheng X, Chu Z, Peng S, Yang D, et al. Mining characteristic microbes in pickled chili peppers. NPJ Sci Food. 2025;9:77. https://doi.org/10.1038/s41538-025-00442-7

Xu J, Kong J, Zheng X, Chu Z, Osei PO, Peng W, et al. Novel metabolites linked to Lactiplantibacillus plantarum in chili fermentation. J Future Foods. 2025. https://doi.org/10.1016/j.jfutfo.2025.02.011

Shi H, An F, Lin H, Li M, Wu J, Wu R. Advances in fermented foods revealed by multi-omics. Front Microbiol. 2022;13:1044820. https://doi.org/10.3389/fmicb.2022.1044820

Abbaspour N. Fermentation’s role in shaping plant-based foods. Appl Food Res. 2024;4(2):100468. https://doi.org/10.1016/j.afres.2024.100468

Thorakkattu P, Khanashyam AC, Shah K, Babu KS, Mundanat AS, Deliephan A, et al. Postbiotics: current trends in food and pharmaceutical industries. Foods. 2022;11(19):3094. https://doi.org/10.3390/foods11193094

Downloads

Published

12.12.2025

How to Cite

Shahabudin, S., Masri, M. F., & Azmi, N. S. (2025). Probiotics and Postbiotics from Southeast Asian Traditional Fermented Foods Highlight Functional Potential in Malaysia. Journal of Biochemistry, Microbiology and Biotechnology, 13(2), 138–144. https://doi.org/10.54987/jobimb.v13i2.1157

Issue

Section

Articles