Comparative Effects of Freeze-Drying and Spray-Drying on the Physicochemical, Microbiological, and Sensory Properties of Coconut Milk and Dairy Milk Yoghurts
DOI:
https://doi.org/10.54987/jobimb.v13i2.1142Keywords:
Coconut-based yoghurt, Probiotic viability, Functional food, Lactic acid bacteria, Spray and Freeze dryinAbstract
Coconut milk yoghurt, rich in healthy fats, offers a sustainable alternative to dairy. Converting it into powder enhances shelf life, versatility, and probiotic retention for diverse food applications. This study evaluates the effects of spray-drying (SD) and freeze-drying (FD) on the physicochemical, microbiological, and sensory properties of coconut milk-based yoghurt (CMY) compared to dairy yoghurt (DY). Yoghurt powders were prepared by diluting the yoghurts with 64% distilled water and 6% maltodextrin for SD processing, conducted at 150°C inlet and 65–70°C outlet temperatures. For FD, liquid yoghurts were frozen overnight and lyophilized under vacuum at -50°C for four days. Analysis revealed that dried CMY exhibited significantly higher soluble solids and lactic acid levels than dried DY (<0.72%) in both drying treatments. Although protein content in dried CMY powders is significantly lower (<23%) compared to DY powders (27%), the LAB counts were consistently higher in CMY samples. Significant differences were noted in colour parameters (b*), though no differences were observed for L* and a*. All dried yoghurts produced microbiologically safe powders, with yeast, mould, and coliform counts within acceptable limits. Sensory evaluation by 60 panellists using a 9-point hedonic scale indicated greater acceptance for CMY over DY, with SD powders preferred over FD powders. In conclusion, CMY is more preferred over DY for producing high-quality yoghurt powders with enhanced probiotic retention, better sensory appeal, and improved functional properties.
References
Warren V, Bell R, Bruning-Mescher S. Plant-based milk alternatives: consumer needs and marketing strategies. In: Bertella G, Santini C, editors. Plant-Based Food Consumption. Cambridge: Woodhead Publ.; 2024. p. 153–77. https://doi.org/10.1016/B978-0-323-98828-5.00006-1
D’Andrea AE, Kinchla AJ, Nolden AA. A comparison of the nutritional profile and nutrient density of commercially available plant-based and dairy yogurts in the United States. Front Nutr. 2023;10:1195045. https://doi.org/10.3389/fnut.2023.1195045
Clegg ME, Tarrado Ribes A, Reynolds R, Kliem K, Stergiadis S. A comparative assessment of the nutritional composition of dairy and plant-based dairy alternatives available for sale in the UK and the implications for consumers’ dietary intakes. Food Res Int. 2021;148:110586. https://doi.org/10.1016/j.foodres.2021.110586
Beegum PPS, Ramesh SV, Pandiselvam R, Neema M, Daliyamol, Manikantan MR, Hebbar KB. Perspectives on the cardioprotective, neuroprotective and anti-obesity functions of coconut (Cocos nucifera L.). Food Biosci. 2024;58:103756. https://doi.org/10.1016/j.fbio.2024.103756
Nowak D, Jakubczyk E. The freeze-drying of foods: the characteristic of the process course and the effect of its parameters on the physical properties of food materials. Foods. 2020;9(10):1488. https://doi.org/10.3390/foods9101488
Ismail EA, Aly AA, Atallah AA. Quality and microstructure of freeze-dried yoghurt fortified with additives as protective agents. Heliyon.2020;6(10):e05196. https://doi.org/10.1016/j.heliyon.2020.e05196
Santos G, Nogueira RI, Rosenthal A. Powdered yoghurt produced by spray drying and freeze drying: a review. Braz J Food Technol. 2018;21:e2017062. https://doi.org/10.1590/1981-6723.12716
Silva GS, Nunes TP, Rosenthal A, Pagani AA. Development and acceptance of freeze-dried yogurt powder. Int Food Res J. 2018;25:1159–65.
Samborska K, Barańska A, Boostani S, Riazi M, Jafari SM. Introduction to the spray drying process. In: Jafari SM, Samborska K, editors. Spray Drying for the Food Industry. Cambridge: Woodhead Publ.; 2024. p. 3–28. https://doi.org/10.1016/B978-0-12-819799-8.00013-2
Hewlings S. Coconuts and health: different chain lengths of saturated fats require different consideration. J Cardiovasc Dev Dis. 2020;7(4):49. https://doi.org/10.3390/jcdd7040059
Pachekrepapol U, Kokhuenkhan Y, Ongsawat J. Formulation of yogurt-like product from coconut milk and evaluation of physicochemical, rheological, and sensory properties. Int J Gastr Food Sci. 2021;25:100393. https://doi.org/10.1016/j.ijgfs.2021.100393
Koç B, Sakin-Yilmazer M, Balkir P, Kaymak-Ertekin F. Spray drying of yogurt: optimization of process conditions for improving viability and other quality attributes. Dry Technol. 2010;28:495–507. https://doi.org/10.1080/07373931003613809
Mat Sarif SN, Tang JYH, Abd Ghani A. Physicochemical properties and microbial count of bacterial survival in freeze-dried goat milk yogurt powder. J Agrobiotechnol. 2022;13(1S):74–84. https://doi.org/10.37231/jab.2022.13.1S.317
AOAC. AOAC official method 930.15. Loss on drying (moisture) for feeds (at 135 °C for 2 h): dry matter on oven drying for feeds (at 135 °C for 2 h). In: Latimer GW Jr, editor. Offic Methods Anal AOAC Int. Oxford: Oxford Univ Press; 2023. https://doi.org/10.1093/9780197610145.001.0001
Sadler G, Murphy P. pH and titratable acidity. In: Food Analysis. 2010. p. 219–38. https://doi.org/10.1007/978-1-4419-1478-1_13
Hasan TA, Isa JK. Study of the microbiological quality and physicochemical of soft white cheese in Iraq. Nat Volatiles Essent Oils. 2021;8(6):48–61. DOI not available.
Izadi M, Eskandari MH, Niakousari M, Shekarforoush S, Hanifpour MA, Izadi Z. Optimisation of a pilot-scale spray drying process for probiotic yoghurt using response surface methodology. Int J Dairy Technol. 2014;67(2):211–9. https://doi.org/10.1111/1471-0307.12108
Santiago-García PA, Mellado-Mojica E, León-Martínez FM, Dzul-Cauich JG, López MG, García-Vieyra MI. Fructans (agavins) from Agave angustifolia and Agave potatorum as fat replacement in yogurt: effects on physicochemical, rheological, and sensory properties. LWT. 2021;140:110846. https://doi.org/10.1016/j.lwt.2020.110846
Wiktor A, Nowacka M, Dadan M, Rybak K, Lojkowski W, Chudoba T, Witrowa-Rajchert D. The effect of pulsed electric field (PEF) on drying kinetics, color and microstructure of carrot. Dry Technol. 2016;34:1286–96. https://doi.org/10.1080/07373937.2015.1105813
Chetachukwu S, Thongraung C, Yupanqui C. Development of reduced-fat coconut yoghurt: physicochemical, rheological, microstructural and sensory properties. Int J Dairy Technol. 2019;72(1):1–8. https://doi.org/10.1111/1471-0307.12600
Gengan G, Mohd Zaini NS, Saari N, Meor Hussin AS, Jaafar A, Hasan H, et al. Nutritional and therapeutic benefits of coconut milk and its potential as a plant-based functional yogurt alternative: a review. Food Sci Hum Wellness. 2024;15:100496. https://doi.org/10.26599/FSHW.2024.9250004
Jafari SM, Vakili S, Dehnad D. Production of a functional yogurt powder fortified with nanoliposomal vitamin D through spray drying. Food Bioprocess Technol. 2019;12(7):1220–31. https://doi.org/10.1007/s11947-019-02289-9
Saxena A, Maity T, Raju SP, Bawa A. Degradation kinetics of colour and total carotenoids in jackfruit (Artocarpus heterophyllus) bulb slices during hot air drying. Food Bioprocess Technol. 2012;5:672–9. https://doi.org/10.1007/s11947-010-0409-2
Priya SR. Preparation and quality assessment of yoghurt prepared from dairy milk and coconut (Cocos nucifera L.) milk. CORD. 2016;32:10–5. https://doi.org/10.37833/cord.v32i1.42
Hoxha R, Evstatieva Y, Nikolova D. Physicochemical, rheological, and sensory characteristics of yogurt fermented by lactic acid bacteria with probiotic potential and bioprotective properties. Foods. 2023;12(13):2600. https://doi.org/10.3390/foods12132600
Codex Alimentarius Commission. Codex standard for fermented milks. Rome: FAO; 2003.
Sunitha B, Bhavya J, Arvind G, Sk K. Production of powdered yoghurt by spray drying. Int J Eng Res. 2016;5:100–5.
Alyaqoubi S, Abdullah A, Samudi M, Abdullah Sani N, Addai Z, Musa KH. Study of antioxidant activity and physicochemical properties of coconut milk (pati santan) in Malaysia. J Chem Pharm Res. 2015;7:967–73.
Bharti B, Badshah J, Gandhi S, Beniwal B. A review on comparison between bovine milk and plant-based coconut milk. Int J Food Sci Nutr. 2021;6:374–8.
Konadu L, Dedo D, Acquah-Mensah J, Fagbemi EO, editors. Effect of endosperm maturity on the physicochemical composition and sensory acceptability of coconut milk and yoghurt. Proc Food Sci Conf. 2021;1:25–33. https://doi.org/10.5897/AJFS2023.2268
Jørgensen CE, Abrahamsen RK, Rukke EO, Hoffmann TK, Johansen AG, Skeie SB. Processing of high-protein yoghurt: a review. Int Dairy J. 2019;88:42–59. https://doi.org/10.1016/j.idairyj.2018.08.002
Vanga SK, Raghavan V. How well do plant-based alternatives fare nutritionally compared to cow’s milk? J Food Sci Technol. 2018;55(1):10–20. https://doi.org/10.1007/s13197-017-2915-y
Moore JB, Horti A, Fielding BA. Evaluation of the nutrient content of yogurts: a comprehensive survey of yogurt products in the major UK supermarkets. BMJ Open. 2018;8(8):e021387. https://doi.org/10.1136/bmjopen-2017-021387
Shristi G, Gita B, editors. Study of physicochemical, nutritional and sensory characteristics of paneer and yoghurt prepared from coconut milk. Proc Food Nutr Symp. 2019;2:1–7.
Belewu MA, Belewu KY, Bamidele RA. Cyper-coconut yoghurt: preparation, compositional and organoleptic qualities. Afr J Food Sci Technol. 2010;1(1):1–3.
Hempel A, Borchert N, Walsh H, Choudhury KR, Kerry JP, Papkovsky DB. Analysis of total aerobic viable counts in raw fish by high-throughput optical oxygen respirometry. J Food Prot. 2011;74(5):776–82. https://doi.org/10.4315/0362-028X.JFP-10-352
Nordin Y, Kwan SY, Chang W, Loo Y, Tan C, Sani SN, et al. Evaluation of bacteriological quality of locally produced raw and pasteurised milk in Selangor, Malaysia. Food Res. 2018;3:208–12. https://doi.org/10.26656/fr.2017.3(3).235
Hong Kong Centre for Food Safety. Microbiological guidelines for food (for ready-to-eat food in general and specific food items). Hong Kong: Dept. Food Environ Hyg; 2014.
Francisco B. Microbial ecology and fermentation dynamics of traditional and novel fermented yogurt in Brazil. Int J Food Sci. 2024;7:33–43. https://doi.org/10.47604/ijf.2596
Vitheejongjaroen P, Phettakhu P, Arsayot W, Taweechotipatr M, Pachekrepapol U. The ability of Lacticaseibacillus paracasei MSMC 36-9 strain with probiotic potential to ferment coconut milk and produce a yogurt-type beverage. Beverages. 2024;10(2):30. https://doi.org/10.3390/beverages10020030
Huang W, Dong A, Pham HT, Zhou C, Huo Z, Wätjen AP, et al. Evaluation of the fermentation potential of lactic acid bacteria isolated from herbs, fruits and vegetables as starter cultures in nut-based milk alternatives. Food Microbiol. 2023;112:104243. https://doi.org/10.1016/j.fm.2023.104243
Getachew A, Tadie A, Chercos DH, Guadu T. Level of faecal coliform contamination of drinking water sources and its associated risk factors in rural settings of North Gondar Zone, Ethiopia: a cross-sectional community-based study. Ethiop J Health Sci. 2018;28(2):227–34. https://doi.org/10.4314/ejhs.v28i2.14
Some S, Mondal R, Mitra D, Jain D, Verma D, Das S. Microbial pollution of water with special reference to coliform bacteria and their nexus with environment. Energy Nexus. 2021;1:100008. https://doi.org/10.1016/j.nexus.2021.100008
Martin NH, Trmčić A, Hsieh TH, Boor KJ, Wiedmann M. The evolving role of coliforms as indicators of unhygienic processing conditions in dairy foods. Front Microbiol. 2016;7:1549. https://doi.org/10.3389/fmicb.2016.01549
El-Sayed HS, Salama HH, Edris AE. Survival of Lactobacillus helveticus CNRZ32 in spray-dried functional yogurt powder during processing and storage. J Saudi Soc Agric Sci. 2020;19(7):461–7. https://doi.org/10.1016/j.jssas.2020.08.003
Cardona M, Izquierdo D, Barat JM, Fernández-Segovia I. Intrinsic and extrinsic attributes that influence choice of meat and meat products: techniques used in their identification. Eur Food Res Technol. 2023;249(10):2485–514. https://doi.org/10.1007/s00217-023-04301-1
Yang J, Lee J. Application of sensory descriptive analysis and consumer studies to investigate traditional and authentic foods: a review. Foods. 2019;8(2):54. https://doi.org/10.3390/foods8020054
Grasso N, Alonso-Miravalles L, O’Mahony JA. Composition, physicochemical and sensorial properties of commercial plant-based yogurts. Foods. 2020;9(3):252. https://doi.org/10.3390/foods9030252
Amirah AS, Syazwani S, Shukri R, Anis Sobirin MH, Nor-Khaizura MAR, Ibdalluh W, Ramli N. Influence of raisin puree on the physicochemical properties, resistant starch, probiotic viability and sensory attributes of coconut milk yogurt. Food Res. 2019;4:77–84. https://doi.org/10.26656/fr.2017.4(1).185
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Normaziatul Nadiah Hazkhirie, Gengghatarani Gengan, Nurul Fatin Amirah Mahadzir, Fatin Farhanah Abdullah1 Abdullah, Anis Nabilah Junaidi, Muhamad Hafiz Abd Rahim

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
