Impact of High-Pressure Processing on the Physicochemical and Storage Stability of Tepache, a Pineapple By-product Fermented Beverage

Authors

  • Nor Afizah Mustapha Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Khairina Azreen Zainal Abidin
  • Wan Zunairah Wan Ibadullah Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Nur Hanani Zainal Abedin Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Nor-Khaizura Mahmud Ab Rashid aculty of Food Science and Technology, Universiti Putra Malaysia, 43400

DOI:

https://doi.org/10.54987/jobimb.v13i2.1139

Keywords:

Tepache, Functional Beverage, Fermentation, High-Pressure Processing, Antioxidant

Abstract

Tepache is a functional fermented beverage made from pineapple by-products. It is rich in prebiotics and bioactive compounds. However, prolonged storage of the beverage can lead to further proliferation of microorganisms, eventually resulting in an undesirable taste and changes in nutritional composition. There is a need to extend the shelf life of the tepache without compromising its organoleptic and nutritional properties. In this study, the effect of high-pressure processing (HPP) on the physicochemical properties, microbial quality, and sensory characteristics of tepache was investigated. Samples were treated at 300, 450, and 600 MPa for 6 min and stored at 4 ℃ for 14 days. Total plate count, pH, total soluble solids, color, DPPH scavenging activity, and sensory evaluation were analyzed. HPP significantly reduced the microbial load, ranging from no viable cells to 6.00 log10 CFU/mL, with 600 MPa showing the highest efficacy. Tepache treated with HPP exhibited higher pH, total soluble solids, and sensory scores, which increased with higher pressure. The treatment maintained the color of the sample, but a slight decrease in b* was observed during storage. The DPPH scavenging activity was improved by the high-pressure treatment, and lower reduction was observed during storage compared to the control sample. The findings suggest that HPP at higher pressure is a suitable option to improve both the shelf life and quality of fermented beverages without compromising their antioxidative properties.

Author Biography

Khairina Azreen Zainal Abidin

Tepache is a functional fermented beverage made from pineapple by-products. It is rich in prebiotics and bioactive compounds. However, prolonged storage of the beverage can lead to further proliferation of microorganisms, eventually resulting in an undesirable taste and changes in nutritional composition. There is a need to extend the shelf life of the tepache without compromising its organoleptic and nutritional properties. In this study, the effect of high-pressure processing (HPP) on the physicochemical properties, microbial quality, and sensory characteristics of tepache was investigated. Samples were treated at 300, 450, and 600 MPa for 6 min and stored at 4 ℃ for 14 days. Total plate count, pH, total soluble solids, color, DPPH scavenging activity, and sensory evaluation were analyzed. HPP significantly reduced the microbial load, ranging from no viable cells to 6.00 log10 CFU/mL, with 600 MPa showing the highest efficacy. Tepache treated with HPP exhibited higher pH, total soluble solids, and sensory scores, which increased with higher pressure. The treatment maintained the color of the sample, but a slight decrease in b* was observed during storage. The DPPH scavenging activity was improved by the high-pressure treatment, and lower reduction was observed during storage compared to the control sample. The findings suggest that HPP at higher pressure is a suitable option to improve both the shelf life and quality of fermented beverages without compromising their antioxidative properties.

References

Alvarado C, Garcia B, Martin S, Regalado C. Food-associated lactic acid bacteria with antimicrobial potential from traditional Mexican foods. Rev. Latinoam. Microbiol. 2006; 48: 260-268.

Gutiérrez-Sarmiento W, Peña-Ocaña BA, Lam-Gutiérrez A, Guzmán-Albores JM, Jasso-Chávez R, Ruíz-Valdiviezo VM. Microbial community structure, physicochemical characteristics and predictive functionalities of the Mexican tepache fermented beverage, Microbiol. Res. 2022; 260: 127045. https://doi.org/10.1016/j.micres.2022.127045

Corona-González RI, Ramos-Ibarra JR, Gutiérrez-González P, Pelayo-Ortiz C, Guatemala-Morales GM, Arriola-Guevara E. The use of response surface methodology to evaluate the fermentation conditions in the production of tepache. Rev. Mex. Ing. Quím. 2013; 12(1): 19-28.

de la Fuente-Salcido NM, Castañeda-Ramírez JC, García-Almendárez BE, Bideshi DK, Salcedo-Hernández R, Barboza-Corona, JE. (2015). Isolation and characterization of bacteriocinogenic lactic bacteria from M-Tuba and Tepache, two traditional fermented beverages in México. Food Sci. Nut. 2015; 3(5): 434–442. https://doi.org/10.1002/fsn3.236

Romero-Luna HE, Peredo-Lovillo A, Dávila-Ortiz G. 2022. Tepache: a pre-hispanic fermented beverage as a potential source of probiotic yeasts. Hispanic foods: Chemistry of fermented foods, Chap. 9, In ACS Symposium SeriesVol. 1406. Ilinois: American Chemical Society; 2022: 135-147. https:doi//.org/10.1021/bk-2022-1406.ch009

Ma Y, Yin X, Bi X, Su F, Liang Z, Luo M, Fu D, Xing Y, Che Z. (2019). Physicochemical properties and bioactive compounds of fermented pomegranate juice as affected by high-pressure processing and thermal treatment. Int. J. Food Prop. 2019; 22(1): 1250–1269. https://doi.org/10.1080/10942912.2019.1640737

Arifan F, Winarni S, Wahyuningsih, Pudjihastuti I, Wisnu Broto RTD. Total plate count (TPC) analysis of processed ginger on tlogowungu tourism village. Adv. Eng. Res. 2018; 167: 377-379. https://doi.org/10.2991/icoma-18.2019.80

Choi MH, Kim GH, Lee HS. Effects of ascorbic acid retention on juice color and pigment stability in blood orange (Citrus sinensis) juice during refrigerated storage. Food Res. Int. 2002; 35(8): 753-759. https://doi.org/10.1016/S0963-9969(02)00071-6

Oikeh EI, Omoregie ES, Oviasogie FE, Oriakhi K. Phytochemical, antimicrobial, and antioxidant activities of different citrus juice concentrates. Food Sci. Nut. 2016; 4(1): 103–109. https://doi.org/10.1002/fsn3.268

Xia Q, Liu Q, Cao Y, Zhao Y, Lu S, Wu D, Guan AR. Improving quality of sea buckthorn juice by high-pressure processing. LWT. 2023;185: 115149. https://doi.org/10.1016/j.lwt.2023.115149

Pereira ALF, Almeida FDL, de Jesus ALT. Storage stability and acceptance of probiotic beverage from cashew apple juice. Food Bioprocess Technol. 2013; 6: 3155–3165. https://doi.org/10.1007/s11947-012-1032-1

Abdurrahman FHR, Titik S. Antioxidant activity and organoleptic quality of probiotic tepache of pineapple peel sugar variation and fermentation duration. Proceeding of International Conference on Biology Education, Natural Science, and Technology (INCOBEST), 2023; 1(1): 502-508.

Truffault V, Fry SC, Stevens RG, Gautier H. Ascorbate degradation in tomato leads to accumulation of oxalate, threonate and oxalyl threonate. Plant J. 2017; 89: 996–1008. https://doi.org/10.1111/tpj.13439

Nokthai P, Lee VS, Shank L. Molecular modeling of peroxidase and polyphenol oxidase: substrate specificity and active site comparison. Int. J. Mol. Sci. 2010; 11: 3266–3276. https://doi.org/10.3390/ijms11093266

Sutedjo KSD, Nisa FC. Star fruit (Averrhoa carambola L.) concentrate and fermentation period in physic-chemical microbiology properties of yoghurt. J. Pangan Agroindustri 2015; 3(2): 582–593

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Published

12.12.2025

How to Cite

Mustapha, N. A. ., Abidin, K. A. Z. ., Ibadullah, W. Z. W., Abedin, N. H. Z., & Rashid, N.-K. M. A. (2025). Impact of High-Pressure Processing on the Physicochemical and Storage Stability of Tepache, a Pineapple By-product Fermented Beverage. Journal of Biochemistry, Microbiology and Biotechnology, 13(2), 70–73. https://doi.org/10.54987/jobimb.v13i2.1139

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