Valorization of Pineapple By-products (Ananas comosus) to Enhance Proximate and Antioxidant Properties of Green Tea Kombucha

Authors

  • Nur Aqilah Mohamad Arif Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Siti Nur Syahirah Rosli Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Mohd Sabri Pak-Dek Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Nurul Shazini Ramli Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

DOI:

https://doi.org/10.54987/jobimb.v13i2.1136

Keywords:

Kombucha, Pineapple by-product, Antioxidant activity, Sustainability, Symbiotic Culture of Bacteria and Yeast (SCOBY)

Abstract

The shift towards health-conscious lifestyles has significantly influenced the functional foods market, leading to increased innovation in the development of fermented beverages. Pineapple (Ananas comosus), is recognized for its rich bioactive compounds such as bromelain, and dietary fiber, making their utilization not only nutritionally beneficial but also sustainable. Previous studies have shown that the fusion of pineapple with black tea increased the antioxidant properties of kombucha. However, the effects of pineapple infusion and its by-products on the proximate composition and antioxidant activities of green tea kombucha remain unclear. Therefore, this study aimed to investigate the proximate composition and antioxidant of green tea kombucha when infused with dried pineapple flesh (KPF) and its peels and cores (KPC). The green tea kombucha without any pineapple infusion was used as the control (KT). Proximate composition was analysed using Association of Official Analytical Chemists (AOAC) International standards meanwhile the antioxidant activity was measured using 2,2°-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and Ferric Reducing Antioxidant Power (FRAP) assays. All analyses were conducted on day 13 of fermentation. Findings showed that the infusion with pineapple peels and cores (KPC) in green tea kombucha increased the crude fiber content, probably due to the release of fibrous microparticles during fermentation.  Moreover, KPC had significantly the highest antioxidant activity with an IC50 value of 443.9 ug/mL for ABTS and FRAP value of 77.82 μg TEAC/mL compared to other samples. The findings indicate that KPC has a strong potential as a functional ingredient. Future studies could focus on optimizing the formulation to enhance consumer acceptance of pineapple peel-infused green tea kombucha.

References

de Matos Santos L, Roselino MN, de Carvalho Alves J, Viana SN, dos Reis Requião E, dos Santos JM, et al. Production and characterization of kombucha-like beverage by cocoa (Theobroma cacao) by-product as raw material. Future Foods. 2025;1(11):100528. https://doi.org/10.1016/j.fufo.2024.100528

Jayabalan R, Waisundara VY. Kombucha as a functional beverage. In: Grumezescu AM, Holban AM, editors. Functional and Medicinal Beverages. Cambridge: Woodhead Publ.; 2019. p. 413–46. https://doi.org/10.1016/B978-0-12-816397-9.00012-1

Baek JS, Nam Y, Kim S, Kim HS, Lee EJ, Lee MR, et al. Development of low-caffeine kombucha using lotus root tea and an evaluation of its functional properties. Beverages. 2025;11(2):55. https://doi.org/10.3390/beverages11020055

Treviño-Garza M, Guerrero-Medina A, González-Sánchez R, García-Gómez C, Guzmán-Velasco A, Báez-González J, et al. Production of microbial cellulose films from green tea (Camellia sinensis) kombucha with various carbon sources. Coatings. 2020;10(11):1132. https://doi.org/10.3390/coatings10111132

Teixeira Oliveira J, Machado da Costa F, Gonçalves da Silva T, Dotto Simões G, dos Santos Pereira E, Quevedo da Costa P. Green tea and kombucha characterization: phenolic composition, antioxidant capacity and enzymatic inhibition potential. Food Chem. 2023;408:135206. https://doi.org/10.1016/j.foodchem.2022.135206

Liu Y, Zheng Y, Yang T, Regenstein JM, Zhou P. Functional properties and sensory characteristics of kombucha analogs prepared with alternative materials. Trends Food Sci Technol. 2022;129:608–16. https://doi.org/10.1016/j.tifs.2022.11.001

Sornkayasit K, Jumnainsong A, Srijampa S, Ruknarong L, Buddhisa S, Thanonkeo P, et al. Immunomodulatory potentials of modified kombucha with pineapple by-products in aging: an ex vivo study. J Funct Foods. 2024;112:105933. https://doi.org/10.1016/j.jff.2023.105933

Ahmad Zamri MFM, Akhiar A, Mohd Roslan ME, Mohd Marzuki MH, Saad JM, Shamsuddin AH. Valorisation of organic fraction of municipal solid waste via anaerobic co-digestion of Malaysian tropical fruits for biogas production. IOP Conf Ser Earth Environ Sci. 2020;476:012077. https://doi.org/10.1088/1755-1315/476/1/012077

Phung LT, Kitwetcharoen H, Chamnipa N, Boonchot N, Thanonkeo S, Tippayawat P, et al. Changes in the chemical compositions and biological properties of kombucha beverages made from black teas and pineapple peels and cores. Sci Rep. 2023;13(1):7859. https://doi.org/10.1038/s41598-023-34954-7

Tan CY, Mohd N, Sabran MR. Banana peels as potential prebiotic and functional ingredient. J Gizi Pangan. 2024;19(Suppl 1):119–26. https://doi.org/10.25182/jgp.2024.19.Supp.1.119-126

AOAC International. Official Methods of Analysis of the Association of Official Analytical Chemists International. 18th ed. Gaithersburg (MD): AOAC Int.; 2005.

Xiao F, Xu T, Lu B, Liu R. Guidelines for antioxidant assays for food components. Food Front. 2020;1(1):60–9. https://doi.org/10.1002/fft2.10

Ramli NS, Ismail P, Rahmat A. Influence of conventional and ultrasonic-assisted extraction on phenolic contents, betacyanin contents, and antioxidant capacity of red dragon fruit (Hylocereus polyrhizus). Sci World J. 2014;2014:1–7. https://doi.org/10.1155/2014/964731

Barakat N, Beaufort S, Rizk Z, Bouajila J, Taillandier P, El Rayess Y. Kombucha analogues around the world: a review. Crit Rev Food Sci Nutr. 2022;62(24):6785–809. https://doi.org/10.1080/10408398.2022.2069673

Meena L, Sengar AS, Neog R, Sunil CK. Pineapple processing waste (PPW): bioactive compounds, their extraction, and utilisation—a review. J Food Sci Technol. 2021;59(11):4152–64. https://doi.org/10.1007/s13197-021-05271-6

Sanwal N, Gupta A, Bareen MA, Sharma N, Sahu JK. Kombucha fermentation: recent trends in process dynamics, functional bioactivities, toxicity management, and potential applications. Food Chem Adv. 2023;3:100421. https://doi.org/10.1016/j.focha.2023.100421

Nordin NL, Sulaiman R, Bakar J, Noranizan MA. Comparison of phenolic and volatile compounds in MD2 pineapple peel and core. Foods. 2023;12(11):2233. https://doi.org/10.3390/foods12112233

Mala T, Piayura S, Itthivadhanapong P. Characterization of dried pineapple (Ananas comosus L.) peel powder and its application as a novel functional food ingredient in cracker product. Future Foods. 2024;1(9):100322. https://doi.org/10.1016/j.fufo.2024.100322

Sethi S, Joshi A, Arora B, Bhowmik A, Sharma RR, Kumar P. Significance of FRAP, DPPH, and CUPRAC assays for antioxidant activity determination in apple fruit extracts. Eur Food Res Technol. 2020;246(3):591–8. https://doi.org/10.1007/s00217-020-03432-z

Dzah CS, Kpodo FM, Asante-Donyinah D, Boateng NA. Influence of Morinda citrifolia fruit maturity level, parts and storage length on total phenols, ascorbic acid, antioxidant activity and ethylene gas emission. Food Chem Adv. 2024;1(4):100599. https://doi.org/10.1016/j.focha.2023.100599

Uslu N, Özcan MM. Determination of bioactive properties and quantitative values of phenolic components of different layers of pineapple fruit. J Food Saf Food Qual. 2024;75(3):86–91. https://doi.org/10.53194/0003-925X-75-86

Han Y, Wang X, Gao Z. Microbial species, metabolites, and natural safety control strategies for harmful factors during the fermentation process of Fu Brick Tea. Food Biosci. 2024;15:104753. https://doi.org/10.106/j.fbio.2024.104753

Krishnamoorthy R, Adisa AR, Periyasamy VS, Ahamed A, Alshuniaber MA, Alshatwi AA. Antioxidant potential of biotransformed green tea catechin metabolites and their impact on peripheral blood mononuclear cells. J King Saud Univ Sci. 2022;34(4):101976. https://doi.org/10.1016/j.jksus.2022.101976

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Published

12.12.2025

How to Cite

Arif, N. A. M., Rosli, S. N. S., Pak-Dek, M. S., & Ramli, N. S. . (2025). Valorization of Pineapple By-products (Ananas comosus) to Enhance Proximate and Antioxidant Properties of Green Tea Kombucha. Journal of Biochemistry, Microbiology and Biotechnology, 13(2), 51–56. https://doi.org/10.54987/jobimb.v13i2.1136

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