Survival of Salmonella in Diced Onions Washed by Different Concentrations of Pineapple Vinegar During Refrigerated Storage
DOI:
https://doi.org/10.54987/jobimb.v13i2.1133Keywords:
Diced onions, Salmonella Typhimurium, Natural preservative, Pineapple vinegar, Antimicrobial propertiesAbstract
Salmonella contamination has been repeatedly linked to diced onions. This study investigated the efficacy of washing solutions using pineapple vinegar at varying concentrations (0%, 5%, and 15%) on the survival of S. Typhimurium in diced onions during refrigerated storage conditions. Briefly, diced onions (50 g) were inoculated with S. Typhimurium at ~ 8 log CFU/g and then submerged in the washing solutions for 2 min. Inoculated onions were allowed to dry for approximately 1 hr, divided into 1 g portions and stored at refrigerated conditions for 12 days. Salmonella was enumerated on Xylose Lysine Deoxycholate (XLD) media at predetermined sampling points during storage. Overall, S. Typhimurium exhibited a little reduction (5.63 ± 0.34 log CFU/g) at 0% washing solution, whereas 6.25 ± 0.53 log CFU/g at 15% pineapple vinegar during the entire storage. Salmonella populations showed moderate reductions at 5% pineapple vinegar washing solution at the beginning of storage, but remained stable throughout the storage period. These findings suggest that pineapple vinegar could serve as a natural alternative to chemical preservatives, providing greater microbiological safety for diced onions while preserving their quality. The study highlights the role of natural antimicrobial agents in enhancing food safety and prolonging the shelf life of fresh produce.
References
Hossain MA, Rahman SM. Total phenolics, flavonoids and antioxidant activity of tropical fruit pineapple. Food Res Int. 2011;44(3):672–6. http://doi.org/10.1016/j.foodres.2010.11.036
Roda A, De Faveri DM, Dordoni R, Lambri M. Vinegar production from pineapple wastes – preliminary saccharification trials. Chem Eng Trans. 2014;37:607–12. http://doi.org/10.3303/CET1437102
Food and Drug Administration (US). Salmonella (Salmonellosis). FDA; 2019 Mar 29. Available from: https://www.fda.gov/food/foodborne-pathogens/Salmonella-salmonellosis Accessed 2024 Jul 4.
Malaysian Society of Infectious Diseases and Chemotherapy. Typhoid. MSIDC; 2024. Available from: https://adultimmunisation.msidc.my/typhoid/ Accessed 2024 Jul 17.
Centers for Disease Control and Prevention (US). Salmonella outbreak linked to cantaloupes. CDC; 2024 Jan 19. Available from: https://www.cdc.gov/Salmonella/sundsvall-11-23/index.html Accessed 2024 Jul 10.
Centers for Disease Control and Prevention (US). Salmonella outbreak linked to cucumbers. CDC; 2024 Jul 2. Available from: https://www.cdc.gov/Salmonella/africana-06-24/index.html Accessed 2024 Jul 10.
Centers for Disease Control and Prevention (US). Salmonella outbreak linked to fresh basil. CDC; 2024 Jun 18. Available from: https://www.cdc.gov/Salmonella/basil-04-24/index.html Accessed 2024 Jul 10.
World Health Organization. Typhoid. WHO; 2023 Mar 30. Available from: https://www.who.int/news-room/fact-sheets/detail/typhoid Accessed 2024 Jul 11.
Centers for Disease Control and Prevention (US). Salmonella outbreak linked to fresh diced onions. CDC; 2023 Dec 13. Available from: https://www.cdc.gov/Salmonella/thompson-10-23/index.html Accessed 2024 Jul 10.
Food and Drug Administration (US). Outbreak investigation of Salmonella Oranienburg: whole, fresh onions. FDA; 2022 Feb 2. Available from: https://www.fda.gov/food/outbreaks-foodborne-illness/outbreak-investigation-Salmonella-oranienburg-whole-fresh-onions-october-2021 Accessed 2024 Jul 4.
Food and Drug Administration (US). Separating food – food safety for moms to be. FDA; 2018 Sep 27. Available from: https://www.fda.gov/food/people-risk-foodborne-illness/separating-food-food-safety-moms-be Accessed 2024 Jul 4.
Andino A, Hanning I. Salmonella enterica: survival, colonization, and virulence differences among serovars. Sci World J. 2015;2015:520179. http://doi.org/10.1155/2015/520179
Bertan FA, Marchioro ML, Oldoni TL, Dekker RF, Cunha MA. Valorization of pineapple processing residues through acetification to produce specialty vinegars enriched with red-Jambo extract of Syzygium malaccense leaf. Sci Rep. 2022;12(1):23968. http://doi.org/10.1038/s41598-022-23968-2
Ezenekwe E, Ekwegbalu E, Uchendu AC, Ezemba AS, Osuala OJ, Ezemba CC. Antimicrobial evaluation of vinegar produced from pineapple and pawpaw fruits with their peels. Eur J Biomed Pharm Sci. 2021;8(7):5–11.
Selvanathan Y, Masngut N. Optimization of process factor and characterization of vinegar-like beverage production via spontaneous fermentation from pineapple peel waste. LWT. 2023;182:114818. http://doi.org/10.1016/j.lwt.2023.114818
Soemarie YB, Milanda T, Barliana MI. Fermented foods as probiotics: a review. J Adv Pharm Technol Res. 2021;12(4):335–9. http://doi.org/10.4103/japtr.japtr_116_21
González Hernández MA, Canfora EE, Jocken WE, Blaak EE. The short-chain fatty acid acetate in body weight control and insulin sensitivity. Nutrients. 2019;11(8):1943. http://doi.org/10.3390/nu11081943
Roda A, De Faveri DM, Giacosa S, Dordoni R, Lambri M. Effect of pre-treatments on the saccharification of pineapple waste as a potential source for vinegar production. J Clean Prod. 2016;112:4477–84. http://doi.org/10.1016/j.jclepro.2015.07.019
Cornish P. Ideal temperature to store onions to avoid soft and soggy vegetables. Express. 2023 Mar 2. Available from: https://www.express.co.uk/life-style/food/1741353/how-to-store-onions-temperature Accessed 2024 Jul 6.
Zaher SM, Fujikawa H. Effect of native microflora on the growth kinetics of Salmonella Enteritidis strain 04-137 in raw ground chicken. J Food Prot. 2011;74(5):735–42. http://doi.org/10.4315/0362-028X.JFP-10-334
Chen Q, Song Y, An Y, Lu Y, Zhong G. Soil microorganisms: their role in enhancing crop nutrition and health. Diversity. 2024;16(12):734. http://doi.org/10.3390/d16120734
Shin H, Lee K, Back G, Lee C, Han K. Identification of Fusarium basal rot pathogens of onion and evaluation of fungicides against the pathogens. Mycobiology. 2023;51(4):264. http://doi.org/10.1080/12298093.2023.2243759
Aryal S. Xylose lysine deoxycholate agar: principle, uses, composition, preparation and colony characteristics. Microbiology Info; 2022 Aug 10. Available from: https://microbiologyinfo.com/xylose-lysine-deoxycholate-xld-agar-principle-uses-composition-preparation-and-colony-characteristics/ Accessed 2024 Oct 17.
Bonnet M, Lagier JC, Raoult D, Khelaifia S. Bacterial culture through selective and non-selective conditions: the evolution of culture media in clinical microbiology. New Microbes New Infect. 2020;34:100622. http://doi.org/10.1016/j.nmni.2019.100622
Mardani N, Jahadi M, Sadeghian M, Keighobadi K, Khosravi-Darani K. Antimicrobial activities, phenolic and flavonoid contents, antioxidant and DNA protection of the internal and outer layers of Allium cepa L. from Iran. NFS J. 2023;31:93–101. http://doi.org/10.1016/j.nfs.2023.03.003
Sharma K, Mahato N, Lee YR. Systematic study on active compounds as antibacterial and antibiofilm agent in aging onions. J Food Drug Anal. 2018;26(2):518–28. http://doi.org/10.1016/j.jfda.2017.06.009
Pezzuto A, Belluco S, Losasso C, Patuzzi I, Bordin P, Piovesana A, et al. Effectiveness of washing procedures in reducing Salmonella enterica and Listeria monocytogenes on a raw leafy green vegetable (Eruca vesicaria). Front Microbiol. 2016;7:1663. http://doi.org/10.3389/fmicb.2016.01663
Balogun MA, Sobande OS, Oyeyinka SA. Antimicrobial properties of onion and garlic extracts in beef and chicken. Food Chem Adv. 2023;3:100519. http://doi.org/10.1016/j.focha.2023.100519
Hua S, Wang Y, Wang L. Regulatory mechanisms of acetic acid, ethanol and high temperature tolerances of acetic acid bacteria during vinegar production. Microb Cell Fact. 2024;23:324. http://doi.org/10.1186/s12934-024-02602-y
Álvarez-Ordóñez A, Prieto M, Bernardo A, Hill C, López M. The acid tolerance response of Salmonella spp.: an adaptive strategy to survive in stressful environments prevailing in foods and the host. Food Res Int. 2012;45(2):482–92. http://doi.org/10.1016/j.foodres.2011.04.002
Elpers L, Kretzschmar J, Nuccio P, Bäumler AJ, Hensel M. Factors required for adhesion of Salmonella enterica serovar Typhimurium to corn salad (Valerianella locusta). Appl Environ Microbiol. 2020;86(8):e02757–19. http://doi.org/10.1128/AEM.02757-19
Zhang Y, Keller SE, Grasso-Kelley EM. Fate of Salmonella throughout production and refrigerated storage of tahini. J Food Prot. 2017;80(6):940–6. http://doi.org/10.4315/0362-028X.JFP-16-507
Elpers L, Deiwick J, Hensel M. Effect of environmental temperatures on proteome composition of Salmonella enterica serovar Typhimurium. Mol Cell Proteomics. 2022;21(8):100265. http://doi.org/10.1016/j.mcpro.2022.100265
Zinn K, Bockmühl D. Did granny know best? Evaluating the antibacterial, antifungal and antiviral efficacy of acetic acid for home care procedures. BMC Microbiol. 2020;20:265. http://doi.org/10.1186/s12866-020-01948-8
Leekha S, Terrell CL, Edson RS. General principles of antimicrobial therapy. Mayo Clin Proc. 2011;86(2):156–67. http://doi.org/10.4065/mcp.2010.0639
Winfield MD, Groisman EA. Role of nonhost environments in the lifestyles of Salmonella and Escherichia coli. Appl Environ Microbiol. 2003;69(7):3687–94. http://doi.org/10.1128/AEM.69.7.3687-3694.2003
Al-Lahham S, Sbieh R, Jaradat N, Almasri M, Mosa A, Hamayel A, Hammad F. Antioxidant, antimicrobial and cytotoxic properties of four different extracts derived from the roots of Nicotiana tabacum L. Eur J Integr Med. 2019;33:101039. http://doi.org/10.1016/j.eujim.2019.101039
Vatansever F, Avci P, Vecchio D, Sadasivam M, Gupta A, Chandran R, et al. Antimicrobial strategies centered around reactive oxygen species: bactericidal antibiotics, photodynamic therapy and beyond. FEMS Microbiol Rev. 2013;37(6):955–89. http://doi.org/10.1111/1574-6976.12026
Food and Drug Administration (US). Outbreak investigation of Salmonella: sprouts. FDA; 2023 Jun 9. Available from: https://www.fda.gov/food/outbreaks-foodborne-illness/outbreak-investigation-Salmonella-sprouts-december-2022
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Copyright (c) 2025 Izzhani Sofea Najwa Izzudin, Yashini Selvanathan, Nasratun Masngut, Nurul Hawa Ahmad
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