Neuroprotective Mechanisms of Orientin against Hydrogen Peroxide-induced Oxidative Damage in SH-SY5Y Cells

Authors

  • Kit Ying Lim School of Postgraduate Studies and Research, International Medical University, Kuala Lumpur, Malaysia.
  • Andrew Octavian Sasmita Division of Applied Biomedical Sciences and Biotechnology, School of Health Sciences, International Medical University, Kuala Lumpur, Malaysia.
  • Anna Pick Kiong Ling Division of Applied Biomedical Sciences and Biotechnology, School of Health Sciences, International Medical University, Kuala Lumpur, Malaysia.
  • Rhun Yian Koh Division of Applied Biomedical Sciences and Biotechnology, School of Health Sciences, International Medical University, Kuala Lumpur, Malaysia
  • Kenny Gah Leong Voon Division of Pathology, School of Medicine, International Medical University, Kuala Lumpur, Malaysia.
  • Yee How Say Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman Perak Campus, Perak, Malaysia.
  • Ying Pei Wong Division of Applied Biomedical Sciences and Biotechnology, School of Health Sciences, International Medical University, Kuala Lumpur, Malaysia.

DOI:

https://doi.org/10.54987/jobimb.v6i1.384

Keywords:

Orientin, Neuroprotective, Nrf2/Keap-1, PI3K/Akt, MAPK/ERK

Abstract

Natural products or plant derivatives could be used to prevent or treat neurodegenerative diseases (ND).  Oxidative stress has been highly implicated in the progression of ND;thus, this leads to the study on the effects of orientin in regulating Nrf2/Keap-1 redox signalling, PI3K/Akt survival and MAPK/ERK apoptosis pathways in the hydrogen peroxide (H2O2)-induced oxidative damage in SH-SY5Y cells. The cells were treated with half maximum non-toxic dose (½ MNTD) or maximum non-toxic dose (MNTD) of orientin and subsequently with H2O2. Cells were then subjected to the measurement of nitric oxide (NO), intracellular calcium (Ca2+), mitochondrial membrane potential (MMP) and annexin V/propidium iodide apoptosis assays. Regulation of the signalling pathways was analysed by Western blotting. Results showed that ½ MNTD of orientin reduced the NO levels. The intracellular Ca2+ level was also reduced by ½ MNTD and MNTD of orientin. The orientin treatment at ½ MNTD and MNTD restored the loss of MMP. Pre-treatment of orientin reduced the early and late apoptotic cells as well as necrotic cells. Orientin was found to up-regulate the Nrf2/Keap-1 and PI3K/Akt pathways whilst down-regulate the MAPK/ERK pathway. The findings from this study will be useful for the prevention of ND in the near future.

 

 

 

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Published

31.07.2018

How to Cite

Lim, K. Y., Sasmita, A. O., Ling, A. P. K., Koh, R. Y., Voon, K. G. L., Say, Y. H., & Wong, Y. P. (2018). Neuroprotective Mechanisms of Orientin against Hydrogen Peroxide-induced Oxidative Damage in SH-SY5Y Cells. Journal of Biochemistry, Microbiology and Biotechnology, 6(1), 10–18. https://doi.org/10.54987/jobimb.v6i1.384

Issue

Vol. 6 No. 1 (2018)

Section

Articles

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