Biodegradation of Synthetic Reactive Orange 16 Dye in Anaerobic Reactor Conditions
DOI:
https://doi.org/10.54987/jobimb.v11i1.815Abstract
Industrial use of azo dyes has been linked to severe environmental damage in the textile and apparel sectors. The breakdown products of many azo dyes are poisonous and mutagenic, posing a threat to human health and the environment. Hence, azo dye wastewater bioremediation is gaining interest. The objective of this study was focused on the biodegradation of an azo dye, Reactive Orange 16 (RO16), in an anaerobic continuous tank reactor operated with digested sludge sample. The digested sludge was used as the organic biodegradation agent for azo dye. The aim was to investigate the fate of the anaerobic RR16 breakdown products during the degradation process. Gas Chromatography-Mass Spectrophotometry (GC-MS) was used to identify RO16 biodegradation metabolites and their corresponding molecular weight. As a result, RO 16 showed about 85.91% decolorization capacity, and the degradation metabolites were detected as pentanoic acid and phenol after seven days of incubation. The degradation mechanism was completed as the azo bond of RO16 was successfully cleaved based on the result of GC-MS, and the proposed pathway of the degradation mechanism is described. The digested palm oil sludge with dye degradation enzymes may provide new avenues for biological waste treatment, especially wastewater bioremediation.
References
Zhu Y hai, Yuan S, Bao D, Yin Y bin, Zhong H xia, Zhang X bo, et al. Decorating waste cloth via industrial wastewater for tube-type flexible and wearable sodium-ion batteries. Adv Mater. 2017;29(16):1603719.
Gao Y, Yang B, Wang Q. Biodegradation and decolorization of dye wastewater: A Review. IOP Conf Ser Earth Environ Sci. 2018 Aug;178(1):012013.
Kishor R, Purchase D, Saratale GD, Saratale RG, Ferreira LFR, Bilal M, et al. Ecotoxicological and health concerns of persistent coloring pollutants of textile industry wastewater and treatment approaches for environmental safety. J Environ Chem Eng. 2021 Apr 1;9(2):105012.
Yaser AZ, Pogaku R. Recent trends for the removal of colored particles in industrial wastewaters. Environ Sci Pollut Res Int. 2017 Jul;24(19):15861-2.
Shahi A, V C P, Singh RS, Verma A. Biodegradation of reactive red 120 in microbial fuel cell by Staphylococcus equoruma RAP2: Statistical modelling and process optimization. J Water Process Eng. 2021 Apr 1;40:101913.
Wielewski LP, Zuccolotto T, Soares M, Prola LDT, Liz MV de. Degradation of the textile dye Reactive Black 5 by Basidiomycetes. Rev Ambiente Água. 2020 Feb 10;15:e2464.
El-Naggar NEA, Hamouda RA, Saddiq AA, Alkinani MH. Simultaneous bioremediation of cationic copper ions and anionic methyl orange azo dye by brown marine alga Fucus vesiculosus. Sci Rep. 2021 Feb 11;11(1):3555.
Firoozeh F, Shahamat Y, Rodriguez-Couto S, Kouhsari E, Niknejad F. Bioremediation for the decolorization of textile dyes by bacterial strains isolated from dyeing wastewater. Jordan J Biol Sci. 2022 Jun 1;15.
Akansha K, Yadav AN, Kumar M, Chakraborty D, Ghosh Sachan S. Decolorization and degradation of reactive orange 16 by Bacillus stratosphericus SCA1007. Folia Microbiologica. 2022 Feb 1:1-2.
Saha P, Sivaramakrishna A, Rao KV. Bioremediation of reactive orange 16 by industrial effluent-adapted bacterial consortium VITPBC6: process optimization using response surface methodology (RSM), enzyme kinetics, pathway elucidation, and detoxification. Environmental Science and Pollution Research. 2023 Mar;30(12):35450-77.
Jamee R, Siddique R. Biodegradation of Synthetic dyes of textile effluent by microorganisms: An environmentally and economically sustainable approach. Eur J Microbiol Immunol. 2019 Dec 25;9(4):114-8.
Telke A, Kadam A, Govindwar S. Bacterial enzymes and their role in decolorization of azo dyes. In: Environmental Science and Engineering (Subseries: Environmental Science). 2014. p. 149-68.
Saratale RG, Saratale GD, Chang JS, Govindwar SP. Bacterial decolorization and degradation of azo dyes: A review. J Taiwan Inst Chem Eng. 2011;42(1):138-57.
Latif MAS, Ariffin F, Rudiyanto, Ismail S, Umor N, Yaser AZ, et al. Decolorization of synthetic azo dyes under anaerobic condition in a continuous stirred tank reactor. J Pure Appl Microbiol. 2021 Jun 1;15(2):658-66.
Cui D, Li G, Zhao M, Han S. Decolourization of azo dyes by a newly isolated Klebsiella sp. strain Y3, and effects of various factors on biodegradation. Biotechnol Biotechnol Equip. 2014 May 4;28(3):478-86.
Fernandes CD, Nascimento VRS, Meneses DB, Vilar DS, Torres NH, Leite MS, et al. Fungal biosynthesis of lignin-modifying enzymes from pulp wash and Luffa cylindrica for azo dye RB5 biodecolorization using modeling by response surface methodology and artificial neural network. J Hazard Mater. 2020;399:123094.
Krithika A, Gayathri KV, Kumar DT, Doss CGP. Mixed azo dyes degradation by an intracellular azoreductase enzyme from alkaliphilic Bacillus subtilis: a molecular docking study. Arch Microbiol. 2021;
Brás R, Isabel M, Pinheiro HM, Gonçalves IC. Batch tests for assessing decolourisation of azo dyes by methanogenic and mixed cultures. In: Journal of Biotechnology. 2001. p. 155-62.
Mubarak NSA, Jawad AH, Nawawi WI. Equilibrium, kinetic and thermodynamic studies of Reactive Red 120 dye adsorption by chitosan beads from aqueous solution. Energy Ecol Environ. 2017;2(1):85-93.
Mani P, Fidal VT, Bowman K, Breheny M, Chandra TS, Keshavarz T, et al. Degradation of Azo Dye (Acid Orange 7) in a microbial fuel cell: Comparison between anodic microbial-mediated reduction and cathodic laccase-mediated oxidation. Front Energy Res. 2019;7(September):1-12.
Šlosar?íková P, Plachá D, Malachová K, Rybková Z, Novotný ?. Biodegradation of Reactive Orange 16 azo dye by simultaneous action of Pleurotus ostreatus and the yeast Candida zeylanoides. Folia Microbiol (Praha). 2020 Aug;65(4):629-38.
Patel H, Yadav VK, Yadav KK, Choudhary N, Kalasariya H, Alam MM, Gacem A, Amanullah M, Ibrahium HA, Park JW, Park S. A recent and systemic approach towards microbial biodegradation of dyes from textile industries. Water. 2022 Oct 8;14(19):3163.
Liu W, Liu C, Liu L, You Y, Jiang J, Zhou Z, et al. Simultaneous decolorization of sulfonated azo dyes and reduction of hexavalent chromium under high salt condition by a newly isolated salt-tolerant strain Bacillus circulans BWL1061. Ecotoxicol Environ Saf. 2017 Jul;141:9-16.
Guo G, Li X, Tian F, Liu T, Yang F, Ding K, et al. Azo dye decolorization by a halotolerant consortium under microaerophilic conditions. Chemosphere. 2020 Apr;244:125510.
Carabajal M, Perullini M, Jobbágy M, Ullrich R, Hofrichter M, Levin L. Removal of phenol by immobilization of trametes versicolor in silica-alginate-fungus biocomposites and Loofa Sponge. Clean - Soil Air Water. 2016;
Chauhan PS, Goradia B, Saxena A. Bacterial laccase: recent update on production, properties and industrial applications. 3 Biotech. 2017;7(5).
Hom-Diaz A, Passos F, Ferrer I, Vicent T, Blánquez P. Enzymatic pretreatment of microalgae using fungal broth from Trametes versicolor and commercial laccase for improved biogas production. Algal Res. 2016;19:184-8.
Schroyen M, Van Hulle SWH, Holemans S, Vervaeren H, Raes K. Laccase enzyme detoxifies hydrolysates and improves biogas production from hemp straw and miscanthus. Bioresour Technol. 2017;244:597-604.
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