Characterizing the Potentials of Alcaligenes sp. for Acrylamide Degradation
DOI:
https://doi.org/10.54987/jobimb.v11i1.804Abstract
Microorganisms play a crucial role in the detoxification and elimination of harmful substances from the environment. Acrylamide is a major pollutant in soil from the breaking down of pesticide addictive. Slowly but steadily, the used of microbe-mediated acrylamide breakdown as a bioremediation approach has gained attention all around the world. This research was aimed to characterize the potential of previously isolated bacteria for acrylamide degradation. In this research five previously isolated bacteria were screened on minimal salt media (MSM) to assess their potential for utilizing acrylamide as the sole nitrogen source. Of all the isolates, isolate D, identified as Alcaligenes sp., displayed the highest growth on acrylamide supplemented MSM agar medium. The optimum conditions for acrylamide degradation by this bacterium were acrylamide concentration of 600 mg/L, pH 7.0, an inoculum size of 400 µL, an incubation time of 96 h, glucose concentration of 4 g/L and temperature of 35 oC. The ability of this isolate to convert toxic acrylamide into less harmful form is a novel finding, highlighting its significance as a valuable tool for bioremediation of this pollutant.
References
Priya N, Nithyakalyan V, Rashika R. Enterobacter cloacae subsp. dissolvens strain TJ1812 in acrylamide biodegradation. Int J Eng Appl Sci Technol. 2020;5(7):255-61.
Ibrahim A, Khalifa U, Sani A, Gado A, Ismail G, Ibrahim M, et al. Bioremediation: a biological tool for environmental mitigation. Int Res J Mod Eng Technol Sci. 2021 Jan 1;3(1):649-55.
Spencer P, Schaumburg HH. Nervous system degeneration produced by acrylamide monomer. Environ Health Perspect. 1975 Jun 1;11:129-33.
Rusnam, Gusmanizar N. Characterization of An Acrylamide-degrading Bacterium Isolated from Volcanic Soil. J Environ Bioremediation Toxicol. 2022 Aug 5;5(1):32-7.
Gafar AA, Shukor MY. Characterisation of an acrylamide-degrading bacterium and its degradation pathway. J Environ Microbiol Toxicol. 2018 Dec 31;6(2):29-33.
Aisami A, Gusmanizar N. Characterization of an acrylamide-degrading bacterium isolated from hydrocarbon sludge. Bioremediation Sci Technol Res. 2019 Dec 28;7(2):15-9.
Mottram DS, Wedzicha BL, Dodson AT. Acrylamide is formed in the Maillard reaction. Nature. 2002 Oct;419(6906):448-9.
Rifai L, Saleh FA. A Review on Acrylamide in Food: Occurrence, Toxicity, and Mitigation Strategies. Int J Toxicol. 2020 Mar 1;39(2):93-102.
Abubakar A, Gusmanizar N, Rusnam M, Syed MA, Shamaan NA, Shukor MY. Remodelling the Growth Inhibition Kinetics of Pseudomonas sp. Strain DrY Kertih on Acrylamide. Bioremediation Sci Technol Res. 2020 Dec 31;8(2):16-20.
Igisu H, Goto I, Kawamura Y, Kato M, Izumi K. Acrylamide encephaloneuropathy due to well water pollution. J Neurol Neurosurg Psychiatry. 1975;38(6):581-4.
Kusnin N, Syed MA, Ahmad SA. Toxicity, pollution and biodegradation of acrylamide - a mini review. J Biochem Microbiol Biotechnol. 2015 Dec 31;3(2):6-12.
Tareke E, Rydberg P, Karlsson P, Eriksson S, Törnqvist M. Analysis of acrylamide, a carcinogen formed in heated foodstuffs. J Agric Food Chem. 2002;50(17):4998-5006.
Chibueze C, Chioma A, Chikere B. Bioremediation techniques - classification based on site of application?: principles , advantages , limitations and prospects. World J Microbiol Biotechnol. 2016;32(11):1-18.
Tijjani AU, Sufyan AJ, Ibrahim S, Shehu D, Ya'u M, Babagana K, et al. Characterization of Aniline Degradation by A Previously Isolated Molybdenum-reducing Pseudomonas sp. Bioremediation Sci Technol Res. 2021 Dec 31;9(2):17-20.
Chandrashekar V, Chandrashekar C, Shivakumar R, Bhattacharya S, Das A, Gouda B, et al. Assessment of acrylamide degradation potential of Pseudomonas aeruginosa BAC-6 isolated from industrial effluent. Appl Biochem Biotechnol. 2014;173(5):1135-44.
Rahman MFA, Manogaran M, Yakasai HM, Yasid NA, Shukor MY. A Two-Level Factorial Design for Screening Factors that Influence the Growth of Bacillus sp. Strain ZEID-14 on Acrylamide. J Environ Bioremediation Toxicol. 2022 Dec 31;5(2):17-24.
Rahim MBH, Syed MA, Shukor MY. Isolation and characterization of an acrylamide-degrading yeast Rhodotorula sp. strain MBH23 KCTC 11960BP. J Basic Microbiol. 2012;52(5):573-81.
Yu F, Fu R, Xie Y, Chen W. Isolation and characterization of polyacrylamide-degrading bacteria from dewatered sludge. Int J Environ Res Public Health. 2015;12(4):4214-30.
Abdullahi UM, Adamu FA, Manogaran M, Yasid NA, Shukor MY. Isolation and Growth Characterization of an Acrylamide-degrading E. cloacae strain UPM2021a Isolated from a Paddy Field. Bioremediation Sci Technol Res. 2022 Dec 31;10(2):1-7.
Shukor A, Yunus M, Gusmanizar N, Ramli J, Shamaan NA, MacCormack W, et al. Isolation and characterization of an acrylamide-degrading Antarctic bacterium. J Environ Biol. 2009;30(1):107-12.
Shukor MY, Gusmanizar N, Azmi NA, Hamid M, Ramli J, Shamaan NA, et al. Isolation and characterization of an acrylamide-degrading Bacillus cereus. J Environ Biol. 2009;30(1):57-64.
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