Isolation, Characterization and Screening of Potential Lambda-Cyhalothrin-Degrading Bacteria from Cultivated Soil in Moro, Kwara State, Nigeria
DOI:
https://doi.org/10.54987/jobimb.v11i1.801Abstract
The indiscriminate use of Lambda Cyhalothrin (LC), one of the commonly used synthetic pyrethroids (SPs) insecticides in agriculture, has led to the contamination of different environments with potentially serious impacts on the health of humans and animals. This study investigated the presence of LC-degrading bacteria in cultivated soils. Bacteria were isolated from agricultural soil on mineral salt medium (MSM) using an enrichment technique. They were characterized and identified. Their growth in different concentrations (50 mg/L, 75 mg/L, 100 mg/L, and 125 mg/L) of LC was studied, Potential to degrade LC was assessed by the growth of the bacteria in a mineral salts medium containing LC as the sole carbon source over 14 days. Five bacteria able to grow in MSM with LC as the sole source of carbon and on nutrient agar enriched with 100mg/L of LC were isolated. Their growth (turbidity and viable counts of the bacterial cells) increased from the third day till the sixth day, after which it declined till the 14th day. The Lambda Cyhalothrin-degrading bacterial isolates (LCDB) were tentatively identified as Bacillus species, Klebsiella species, Pseudomonas species, and Lysinibacillusspecies. These results indicated that these bacterial isolates are potentially able to degrade LC and can be useful for the remediation of SPs-contaminated agricultural soils.
References
Ensley, S.M. Pyrethrins and pyrethroids. In Veterinary toxicology (pp. 515-520). 2018. Academic Press.
Gammon DW, Liu Z, Chandrasekaran A, El-Naggar SF, Kuryshev YA and Jackson S. Pyrethroid neurotoxicity studies with bifenthrin indicate a mixed type I/II mode of action. Pest Manag Sci. 2019;75:1190-1197.
World Health Organization (WHO). Pesticide evaluation scheme, vector ecology and management. Vol. 3. World Health Organization;Geneva, Switzerland. 2016.
Gu BG, Wang HM, Chen WL, Cai DJ and Shan ZJ. Risk assessment of lambda cyhalothrin on aquatic organisms in paddy field in China. Regul Toxicol Pharma. 2017;4(8):69-74.
He LM, Troiano J, Wang A and Goh K. Environmental chemistry, ecotoxicity, and fate of lambda-cyhalothrin. Rev Environ Contam Toxicol. 2018;19(5):71-91.
Xu H, Li W, Schilmiller AL, Eekelen HV and deVos RCH. Pyrethric acid of natural pyrethrin insecticide: Complete pathway elucidation and reconstitution in Nicotiana benthamiana. New Phytol. 2019;223:751-765.
Hao X, Zhang X, Duan B, Huo S, Lin W, Xia X, and Liu K. Screening and Genome Sequencing Of Deltamethrin-Degrading Bacterium ZJ6. CurrMicrobiol. 2018;75(11):1468- 1476.
Kumar TT and Jahangir HS. Biodegradation of lambda cyhalothrin by Rhodococcus erythropolis. Life Sci Inform. 2018;4(5):192-200.
Liu Y, Tang S, Wang X, Wang X, Tang X, Wu Q, Huang Z and Ding J. A novel thermostable and salt-tolerant carboxylesterase involved in the initial aerobic degradation pathway for Pyrethroids in Glycomyces salinus. J Hazard Mater. 2023;451:131128. doi:10.1016/j.jhazmat.2023-131128.
Fang Y, Xu W, Zhang W, Guang C and Mu W. Microbial elimination of pyrethroids: Specific strains and involved enzymes. Appl Microbiol Biot. 2022;106(21):6915-6932.
Moghaddam NS, Zakaria MP, Omar D, Sijam K and Khakvar R. Effects of imidacloprid on the biodiversity of soil microbes in selected soils of Malaysia. Environ Sci Dev. 2011;4:7-10.
Estefan G, Sommer R and Ryan J. Method of Soil, Plant and Water Analysis:A Manual for the West Asia and North Africa region. Third edition, International Centre for Agricultural Research in Dry Areas. 2013.
Wang BB, Wang CL, Liu WX, Liu X, Hou J, Teng Y, Luo YM and Christie P. Biosurfactant-producing microorganism Pseudomonas sp SB assists the phytoremediation of DDT-contaminated soil by two grass species. Chemosphere. 2017;182:137-142.
Parte SG, Kharat AS and Mohekar AD. Isolation and characterization of dichlorovos degrading bacterial strain Pseudomonas stutzerismk. Res J Life Sci Bioinform Pharma Chem Sci. 2017;2(5):282-288.
Mohamed MS. Degradation of methomyl by the novel bacterial strain Stenotrophomonas maltophilia M I. Electron J Biotechnol. 2009;12 (4):1-6.
Shahzad F, Shafee M, Abbas F, Babar S, Tariq M and Ahmad Z. Isolation and biochemical characterization of Rhizobium meliloti from root nodules of Alfalfa (medico sativa). J Anim Plant Sci. 2012;22(2):522-524.
Arora PK and Bae HL. Bacterial degradation of chlorophenols and their derivatives. Microb Cell Fact. 2014;13(1):1-17.
Bergey DH. Bergey's Manual of Systematic Bacteriology. Firmicutes, Vol. 3. Springer-Verlag, New York, 2009;pp 1-1476.
Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood, WA, Krieg NR and Phillips GB. Manual of Methods for General Bacteriology. Eds:1994;(Pp 524) Published by ASM. ISBN-10-0914826298.
Onuorah S, Ngwu V and Nwankwo J. Lambda cyhalothrin and dichlorvos pesticides degradation potentials of bacteria isolated from agricultural soil in Enugu, Enugu State, Nigeria. Int J Adv Technol Sci. 2021;2(1):1-15.
Liu J, Huang WW, Han HT, She CC and Zhong GH. Characterization of cell free extracts from fenpropathrin-degrading strain Bacillus cereus ZH-3 and its potential for bioremediation of pyrethroid-contaminated soils. Sci Total Environ. 2015;523:50-58.
Zhang H, Zhang YM, Hou ZG, Wang XM, Wang J, Lu ZB, Zhao XF, Sun FJ and Pan HY Biodegradation potential of deltamethrin by the Bacillus cereus strain Y1 in both culture and contaminated Soil. Int J Biodeterior Biodeg. 2016;106:53-59.
Chen SH, Deng YY, Chang CQ, Lee J, Cheng YY, Cui ZN, Zhou JA, He F, Hu MY and Zhang LH. Pathway and kinetics of cyhalothrin biodegradation by Bacillus thuringiensis strain ZS-19. Sci Rep.2015;5:8784-8791.
Tang J, Liu B, Shi Y, Zeng CY, Chen TT, Zeng L and Zhang Q. Isolation and identification of fenvalerate-degrading potential of Bacillus licheniformis CY-012. Biotechnol Biotech. Equip. 2018;32(3):574-582.
Farooq H, Khalid M and Hashmi I. Bioremediation of synthetic pyrethroid by hydrolases of Bacillus aryabhattai and Bacillus circulans derived from indigenous soil. J Environ Treat Technol. 2022;10(3):187-194.
Thatheyus AJ, Alexander D and Selvam G. Synthetic Pyrethroids: Toxicity and Biodegradation. Appl Ecol Environ Sci. 2013;1(3):33-36.
Yang J, Feng Y, Zhan H, Liu J, Yang F, Zhang K, Zhang L and Chen S. Characterization of a pyrethroid-degrading Pseudomonas fulva strain P31 and biochemical degradation pathway of d-Phenothrin. Front Microbiol. 2018;9:1003. doi:10.3389/fmicb.2018.01003.
Tang J, Hu Q, Liu B, Lei D, Chen TT, Sun Q, Zeng CY and Zhang Q. Efficient biodegradation of 3-phenoxybenzoic acid and pyrethroid pesticides by the novel strain Klebsiella pneumoniae BPBA052. Can J Microbiol. 2019;65(11):795-804.
Tang J, Hu Q, Lei D, Wu M, Zeng C and Zhang Q. Characterization of deltamethrin degradation and metabolic pathway by co-culture of Acinetobacter junii LH-1-1 and Klebsiella pneumoniae BPBA052. Appl Microbiol Biotech. Exp. 2020;10:106-117.
Anjos CS, Birolli WG and Porto ALM. Biodegradation of the Pyrethroid pesticide esfenvalerate by a bacterial consortium isolated from Brazilian Savannah. J Braz Chem Soc. 2020;31(8):1654-1660.
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