Assessment of Serum 25-Hydroxy Vitamin D Level Among Hypothyroidism with and without Thyroid Peroxidase Antibodies

Authors

  • Ibrahim Wagea Alla Balla Dalia Department of Clinical Chemistry, Faculty of medical laboratory science, Mashreq university, MHF2+GMQ, Al-Inqaz St, Khartoum North, Sudan.
  • A. Abdrabo Abdelkarim Department of Clinical Chemistry, Faculty of Medical Laboratory Science, Elneelian University, JG37+2RM, 52nd St, Khartoum, Sudan
  • Abulmakarim Mahmoud Mustafa Hafiya Department of Clinical Chemistry, Faculty of Medical Laboratory Science, Sudan International University, GJ32+W3F, Khartoum, Sudan.
  • El-Sadiq Abdoalsamed Ahmed Omsalama Department of Clinical Chemistry, Faculty of Medical Laboratory Science, Sudan International University, GJ32+W3F, Khartoum, Sudan.
  • khalifa Abd El-Majeed Aza Department of Clinical Chemistry, Faculty of Medical Laboratory Science, Sudan International University, GJ32+W3F, Khartoum, Sudan.
  • Zainalabdeen Mustafa Hussain Alaa Department of Clinical Chemistry, Faculty of Medical Laboratory Science, Sudan International University, GJ32+W3F, Khartoum, Sudan.
  • Garba Uba Department of Science Laboratory Technology, College of Science and Technology, Jigawa State Polytechnic, Dutse, PMB 7040, Nigeria.

DOI:

https://doi.org/10.54987/jobimb.v10i2.759

Keywords:

Antithyroid Peroxidase (TPO), Vitamin D deficiency, Thyroid disease, Thyroid Function Tests, Free Thyroxine

Abstract

The association between serum 25-Hydroxy vitamin D (25-OH vitamin D) levels in hypothyroid subjects with and without thyroid peroxidase (TPO) antibodies is controversial. There is increasing evidence that 25-OH vitamin D level is associated with autoimmune diseases. The analytical cross-sectional case-control and hospital-based study were conducted at Professor Al-Mahdi M. Ali Center for Diabetes and Endocrinology in Khartoum from February to October 2020.  Sixty subjects with hypothyroidism were recruited from the follow-up clinic. Thirty age and sex-matched subjects were used as control. Thyroid Function Tests [Thyroid-stimulating hormone (TSH), Free Thyroxine (FT4), and  Free triiodothyronine (FT3)] were measured using TOSOH AIA 360system analyzer, and serum 25-OH vitamin D level was measured using semiautomatic I-Chroma-II reader and thyroid peroxidase antibodies (TPO antibodies) were measured in both cases and controls was estimated using ELISA. The results were analyzed using SPSS version 21. TSH was significantly increased in both TPO antibodies positive and TPO antibodies negative hypothyroid subjects than in the controls (22.3±4.44), (10.5±2.55) (3.42±0.75) with a P-value of 0.000. FT4 has decreased in both TPO antibodies positive and TPO antibodies negative subjects than in the control (0.51±0.21) (0.56±0.19) (2.0±0.55) with a P-value of 0.002. FT3 has significantly decreased in both TPO antibodies positive and TPO antibodies negative subjects than in the control  (0.73±0.31)(0.73±0.49)(3.67±0.81) with a p-value of 0.0001. 25-OH Vitamin D level was lower among hypothyroid subjects than in the control. Subjects with positive TPO antibodies had lower 25-OH vitamin D levels than the  TPO-antibody negative subjects compared to control (7.45±4.50̽) (10.5±7.18)(48.8±10.0) with  P-value 0.000. Females 46(77%) were more than males 14(23%) and geographically most subjects were coming from the central part of Khartoum state.

References

Naeem Z. Vitamin d deficiency- an ignored epidemic. Int J Health Sci. 2010;4(1):V-VI.

Holick MF, Chen TC. Vitamin D deficiency: a worldwide problem with health consequences. Am J Clin Nutr. 2008;87(4):1080S-1086S.

Puzin MN, Razinkin OP, Rushanov MI. Facial nerve neuropathy (review of the literature). Zhurnal Nevropatol Psikhiatrii Im SS Korsakova Mosc Russ 1952. 1991;91(5):112-5.

Vilarrasa N, Vendrell J, Maravall J, Elío I, Solano E, San José P, et al. Is plasma 25(OH) D related to adipokines, inflammatory cytokines and insulin resistance in both a healthy and morbidly obese population? Endocrine. 2010;38(2):235-42.

Parva NR, Tadepalli S, Singh P, Qian A, Joshi R, Kandala H, et al. Prevalence of Vitamin D Deficiency and Associated Risk Factors in the US Population (2011-2012). Cureus. 2018; 6.

J GP, Venkatasamy S, S Pyarejan K, K. J. Vitamin D status in children with autoimmune thyroiditis. Int J Contemp Pediatr. 2017;4(5):1595.

Institute of Medicine (US) Committee to Review Dietary Reference Intakes for Vitamin D and Calcium. Dietary Reference Intakes for Calcium and Vitamin D. Ross AC, Taylor CL, Yaktine AL, Del Valle HB, editors. Washington (DC): National Academies Press (US); 2011 [cited 2022 Jul 14]. (The National Academies Collection: Reports funded by National Institutes of Health).

Jones G. Pharmacokinetics of vitamin D toxicity. Am J Clin Nutr. 2008 Aug;88(2):582S-586S.

Lim K, Thadhani R. Vitamin D Toxicity. Braz J Nephrol. 2020;42(2):238-44.

Matsui MS. Vitamin D Update. Curr Dermatol Rep. 2020;9(4):323-30.

Haussler MR, Livingston S, Sabir ZL, Haussler CA, Jurutka PW. Vitamin D Receptor Mediates a Myriad of Biological Actions Dependent on Its 1,25?Dihydroxyvitamin D Ligand: Distinct Regulatory Themes Revealed by Induction of Klotho and Fibroblast Growth Factor?23. JBMR Plus. 2021;5(1).

Chaker L, Bianco AC, Jonklaas J, Peeters RP. Hypothyroidism. The Lancet. 2017;390(10101):1550-62.

Associate Professor, Department of General Medicine, Medical College, Thiruvananthapuram, Kerala, India, Christopher S. Study on Association between Vitamin D Deficiency and Autoimmune Hypothyroidism. J Med Sci Clin Res. 2019;7(11).

Kumari M, Mahli RK, Verma SK, Kumar V. Evaluation of vitamin D levels in patients with primary hypothyroidism: A cross-sectional study. Int J Clin Biochem Res. 2021;8(3):193-7.

R?c?t?ianu N, Leach NV, Bolboac? SD, Cozma A, Dronca E, Valea A, et al. Vitamin D deficiency, insulin resistance and thyroid dysfunction in obese patients: is inflammation the common link? Scand J Clin Lab Invest. 2018;78(7-8):560-5.

Tamer G, Arik S, Tamer I, Coksert D. Relative Vitamin D Insufficiency in Hashimoto's Thyroiditis. Thyroid. 2011;21(8):891-6.

Shin DY, Kim KJ, Kim D, Hwang S, Lee EJ. Low Serum Vitamin D Is Associated with Anti-Thyroid Peroxidase Antibody in Autoimmune Thyroiditis. Yonsei Med J. 2014;55(2):476.

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Published

31.12.2022

How to Cite

Dalia, I. W. A. B., Abdelkarim, A. A., Hafiya, A. M. M., Omsalama, E.-S. A. A., Aza, khalifa A. E.-M., Alaa, Z. M. H., & Uba, G. (2022). Assessment of Serum 25-Hydroxy Vitamin D Level Among Hypothyroidism with and without Thyroid Peroxidase Antibodies. Journal of Biochemistry, Microbiology and Biotechnology, 10(2), 56–59. https://doi.org/10.54987/jobimb.v10i2.759

Issue

Vol. 10 No. 2 (2022)

Section

Articles

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