Range Findings of Lethal Concentration of Zinc Sulfate Heptahydrate (ZnSO4.7H2O) to Juvenile Red Tilapia

Authors

  • Mohd Lukman Muazzim Rohaidi Department of Environment, Faculty of Forestry and Environment, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Wan Lutfi Wan Johari Department of Environment, Faculty of Forestry and Environment, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Khairul Nizam Mohamed Department of Environment, Faculty of Forestry and Environment, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Nur Adeela Yasid Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • Natrah Fatin Mohd Ikhsan Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

DOI:

https://doi.org/10.54987/jobimb.v10i2.741

Keywords:

Juvenile Red Tilapia, Oreochromis sp, Zinc Sulfate, Lethal concentration, Toxicity

Abstract

An acute toxicity study was conducted to determine the range of lethal concentration of zinc sulfate heptahydrate (ZnSO4.7H2O) to juvenile red tilapia (Oreochromis sp.). The mortality of the fish was recorded at 24, 48, 72, and 96 hours of study. The lethal concentration of ZnSO4.7H2O that caused 50% mortality (LC50) was 48.7, 40.0, 34.6, and 33.1 mg/L for 24, 48, 72, and 96 hours, respectively. Meanwhile, the lethal concentration that caused 99% mortality (LC99) was 116.7, 108.7, 76.2, and 69.9 mg/L for 24, 48, 72, and 96 hours, respectively. The Probit analysis with a 95% confidence interval is used to determine the range of lethal concentration of ZnSO4.7H2O for 24, 48, 72, and 96 hours of toxicity study. The result shows that the lethal concentration of ZnSO4.7H2O decreased over time indicating that the low concentration could become lethal to juvenile red tilapia when exposed for longer periods and act as a preliminary study for further study.

References

Authman M, Zaki M, Khallaf E, Abbas H. Use of Fish as Bio-Indicator of The Effects of Heavy Metals Pollution. J Aquac Res Dev. 2015 Apr 1;J Aquaculture Research and Development:328.

Hogstrand C. Zinc. In: Fish Physiology: Homeostasis and Toxicology of Essential Metal. Academic Press; P. 135-200.

Siddiki Akmna, Khair Mu, Naser Mn, Salam Ma. Biophysicochemical Changes In Nile Tilapia, Oreochromis Niloticus Exposed To Znso4.7h2o and Zncl2 Metal Toxicant. J Innov Pharm Biol Sci. 2018 Apr 5;113-8.

Sires J. A Review of Potential Zinc and Copper Pollution Sources In Kenai River Watershed. Alaska Department of Environmental Conservation; 2017.

European Commision. Opinion of The Scientific Committee for Animal Nutrition on The Use of Zinc In Feeding Stuffs. European Commision (Ec); 2003.

European Food Safety Authority. Scientific Opinion on The Safety and Efficacy of Zinc Compounds (E6) as Feed Additives for All Animal Species (Zinc Acetate, Dihydrate; Zinc Chloride, Anhydrous; Zinc Oxide; Zinc Sulphate, Heptahydrate; Zinc Sulphate, Monohydrate; Zinc Chelate of Amino Acids, Hydrate; Zinc Chelate of Glycine, Hydrate), Based on A Dossier Submitted By Fefana asbl. 2015. Available From: Https://Www.Efsa.Europa.Eu/En/Efsajournal/Pub/4058

Finney Dj. Probit Analysis: A Statistical Treatment of The Sigmoid Response Curve. University Press; 1952. 346 P.

Oecd. Test No. 203: Fish, Acute Toxicity Test. Paris: Organisation for Economic Co-Operation and Development; 2019. Available From: Https://Www.Oecd-Ilibrary.Org/Environment/Test-No-203-Fish-Acute-Toxicity-Test_9789264069961-En

American Society for Testing and Materials International (ASTM International). Standard Guide for Conducting Acute Toxicity Tests on Test Materials With Fishes, Macroinvertebrates, and Amphibians. Available From: Https://Www.astm.Org/E0729-96r14.Html

Almeida Ja, Diniz Ys, Marques Sfg, Faine La, Ribas Bo, Burneiko Rc, Et Al. The Use of The Oxidative Stress Responses as Biomarkers In Nile Tilapia (Oreochromis Niloticus) Exposed To In Vivo Cadmium Contamination. Environ Int. 2002 Mar;27(8):673-9.

Garcia-Santos S, Fontaínhas-Fernandes A, Wilson Jm. Cadmium Tolerance In The Nile Tilapia (Oreochromis Niloticus) Following Acute Exposure: assessment of Some Ionoregulatory Parameters. Environ Toxicol. 2006 Feb;21(1):33-46.

Ayotunde Eo, ofem Bo. Acute and Chronic Toxicity of Pawpaw (Carica Papaya) Seed Powder To Adult Nile Tilapia (Oreochromis Niloticus Linne 1757). Afr J Biotechnol. 2008:7(13).

Firat O, Kargin F. Individual and Combined Effects of Heavy Metals on Serum Biochemistry of Nile Tilapia Oreochromis Niloticus. Arch Environ Contam Toxicol. 2010 Jan;58(1):151-7.

Taweel A, Shuhaimi-Othman M, Ahmad Ak. In Vivo Acute Toxicity Tests of Some Heavy Metals To Tilapia Fish (Oreochromis Niloticus). J Biol Sci. 2013;13(5):365-71.

Abdel-Khalek A Adel, Kadry M, Hamed A, Marie Ma. Ecotoxicological Impacts of Zinc Metal In Comparison To Its Nanoparticles In Nile Tilapia; Oreochromis Niloticus. J Basic Appl Zool. 2015 Oct 1;72:113-25.

Abdel-Tawwab M, El-Sayed Go, Shady Sh. Effects of Dietary Protein Levels and Environmental Zinc Exposure on The Growth, Feed Utilization, and Biochemical Variables of Nile Tilapia, Oreochromis Niloticus (L.). Toxicol Environ Chem. 2012 Aug 1;94(7):1368-82.

Abdel-Tawwab M, El-Sayed Go, Shady Sh. Growth, Biochemical Variables, and Zinc Bioaccumulation In Nile Tilapia, Oreochromis Niloticus (L.) as Affected By Water-Born Zinc Toxicity and Exposure Period. Int Aquat Res. 2016 Sep 1;8(3):197-206.

Ponzoni Rw, Hamzah A, Tan S, Kamaruzzaman N. Genetic Parameters and Response To Selection for Live Weight In The Gift Strain of Nile Tilapia (Oreochromis Niloticus). Aquaculture. 2005 Jun 30;247(1):203-10.

Ponzoni Rw, Khaw Hl, Yee Hy. Gift: The Story Since Leaving Iclarm (Now Known as The Worldfish Center): Socioeconomic, Access and Benefit Sharing and Dissemination aspects. Fridtjof Nanesn Institute; 2010. Available From: Https://Digitalarchive.Worldfishcenter.Org/Handle/20.500.12348/1294

Aldoghachi M, Rahman M, Yusoff I, Azirun M. Acute Toxicity and Bioaccumulation of Heavy Metals In Red Tilapia Fish. 2016 Apr 1;26:507-13.

Abdul Rahman S, Zambry H, Kamarzaman S, Jalal A. The Potential Role of Red Tilapia (Oreochromis Niloticus) Scales: Allergic Reaction Test In Mice. J Appl Pharm Sci. 2013 Oct 1;3:45-50.

Jeram S, Sintes Jmr, Halder M, Fentanes Jb, Sokull-Klüttgen B, Hutchinson Th. A Strategy To Reduce The Use of Fish In Acute Ecotoxicity Testing of New Chemical Substances Notified In The European Union. Regul Toxicol Pharmacol Rtp. 2005 Jul;42(2):218-24.

Rufli H, Springer Ta. Can We Reduce The Number of Fish In The Oecd Acute Toxicity Test? Environ Toxicol Chem. 2011 Apr;30(4):1006-11.

Halmi Mie, Rahim Mbha, Othman Ar. Estimation of Lc50 and Its Confidence Interval for The Effect of Ferrous Sulphate on Catla Catla. J Environ Microbiol Toxicol. 2018 Jul 31;6(1):21-3.

Jaafar Inm, Ahmad Sa, Yasid Na. Estimation of Lc50 and Its Confidence Interval for The Effect of Nano-Zero Valent Iron on The Freshwater Zooplankton Species Daphnia Magna. J Environ Microbiol Toxicol. 2018 Dec 31;6(2):25-8.

Schenker N, Gentleman Jf. on Judging The Significance of Differences By Examining The Overlap Between Confidence Intervals. Am Stat. 2001 Aug 1;55(3):182-6.

Grosell M, Nielsen C, Bianchini A. Sodium Turnover Rate Determines Sensitivity To Acute Copper and Silver Exposure In Freshwater Animals. Comp Biochem Physiol Toxicol Pharmacol Cbp. 2002 Sep;133(1-2):287-303.

Dung Lq, Cuong Nm, Huyen Nt, Cu Nd. Acute Toxicity

Kamaruzzaman By, Akbar B, Jalal Kca, Shahbudin S. Accumulation of Metals In The Gills of Tilapia Fingerlings (Oreochromis Niloticus) From In Vitro Toxicology Study. J Fish Aquat Sci. 2010;5(6):503-9.

Abdel-Tawwab M, El-Sayed Go, Shady Shhh. Acute Toxicity of Water-Born Zinc In Nile Tilapia, Oreochromis Niloticus (L.) Fingerlings. Better Sci Better Fish Better Life Proc Ninth Int Symp Tilapia Aquac Shanghai China 22-24 April 2011. 2011;39-44.

United States Environmental Protection Agency. Methods for Measuring The Acute Toxicity of Effluents and Receiving Waters To Freshwater and Marine Organisms. Washington, Dc: Usepa; 2002. Report No.: Epa-821-R-02-012.

Ezemonye Lin, Ogeleka Df, Okieimen Fe. Lethal Toxicity of Industrial Chemicals To Early Life Stages of Tilapia Guineensis. J Hazard Mater. 2008 Aug 30;157(1):64-8.

Ebrahimpour M, Alipour H, Rakhshah S. Influence of Water Hardness on Acute Toxicity of Copper and Zinc on Fish. Toxicol Ind Health. 2010 Jul 1;26(6):361-5.

Abdel-Warith, A A, Younis, M E, Al-asgah, A N, Et Al. Effect of Zinc Toxicity on Liver Histology of Nile Tilapia, Oreochromis Niloticus. Sci Res Essays. 2011 Aug 26;6(17):3760-9.

Ezeonyejiaku C, Obiakor M. Toxicological Study of Single Action of Zinc on Tilapia Species (Oreochromis Niloticus). online J Anim Feed Res. 2011 Apr 7;1:139-43.

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Published

31.12.2022

How to Cite

Rohaidi, M. L. M., Johari, W. L. W., Mohamed, K. N. ., Yasid, N. A., & Ikhsan, N. F. M. (2022). Range Findings of Lethal Concentration of Zinc Sulfate Heptahydrate (ZnSO4.7H2O) to Juvenile Red Tilapia. Journal of Biochemistry, Microbiology and Biotechnology, 10(2), 1–4. https://doi.org/10.54987/jobimb.v10i2.741

Issue

Vol. 10 No. 2 (2022)

Section

Articles

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