Phytochemical Screening, Proximate Composition and Mineral Element Analysis of Neocarya macrophylla (Gingerbread) Plum and its Effects on Microorganisms

Authors

  • Jamilu Garba Independent Researcher
  • Asime Oba Independent Researcher
  • Anthony Ofili Department of Microbiology, Central Reference Laboratory, Synlab, Lagos, Nigeria.
  • Barka John Veterinary Teaching Hospital, Faculty of Veterinary Medicine, University of Maiduguri, 600104 Maiduguri, Bama Road, Maiduguri, Borno State, Nigeria.
  • Hadiza Isah Independent Researcher
  • Kwata Veronica John Department of Veterinary pathology, Faculty of Veterinary Medicine, University of Maiduguri, 600104 Maiduguri, Bama Road, Maiduguri, Borno State, Nigeria.
  • Jasini Athanda Musa Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Maiduguri, 600104 Maiduguri, Bama Road, Maiduguri, Borno State, Nigeria.

DOI:

https://doi.org/10.54987/jobimb.v10i1.716

Keywords:

Antimicrobial, N. macrophylla, Minerals, Antibiotics, Antifungal

Abstract

The need to increase the production and utilization of locally available food and antimicrobial resources has been discussed at different national and international forum. Fresh Neocarya macrophylla fruits were obtained from Birnin Kebbi central market in Kebbi state and it was transported to Biochemistry Department at Usmanu Danfodiyo University Sokoto, Sokoto State, Nigeria. Where the fruit of N. macrophylla was transformed or crushed through stigmasterol extraction with 70% of methanol. The compound, stigmasterol, indicated varying actions of antimicrobial activity against the microbes tested. Susceptibility test result showed inhibition ranging from 23 mm to 30 mm against all the organisms, which are S. aureus (24 mm), Salmonella Typhimurium (23 mm), P. aeruginosa (26 mm), E. coli (28 mm), Streptococcus pyogenes (25 mm), B. subitilis (23 mm), A. niger (29 mm), C. albicans (24 mm), and C. kruseii (23 mm). Creating zone of inhibition. The ash content was 6.70±0.05., moisture 14.23%±0.10., lipids 6.70% ±0.05., fibre 10.15%± 0.57., crude protein 1.015%±0.127., carbohydrate 51.33%±1.025. The pulp also contains low concentrations of magnesium (2.843±0.025) and very low concentrations of iron (0.0856±0.002), manganese (0.0122±0.048), copper (0.0087±0.002), and zinc (0.0024±0.001), which are important micro elements required by body for proper functioning. The result obtained indicate that Neocarya macrophylla fruit pulp of pharmaceutical and medical significances that are useful in Combating antibiotics resistance infections, nutritional rich in terms of minerals and carbohydrate composition.

References

CDC Antimicrobial Resistance Coordination and Strategy Unit. (2022, March 2). What CDC is doing: Investments and action. Antibiotic/Antimicrobial Resistance. https://www.cdc.gov/drugresistance/solutions-initiative/index.htmL

Dar K.B. et al., () Efficacy of aqueous and methanolic Extracts of Rheum spiciformis against pathogenic Bacterial and Fungal strains. J Clin Diagnost Res. 2016;10(9):18-22.

Aberoumand, A. A Comparative Study of Nutrients and Mineral Molar Ratios of Some Plant Foods with Recommended Dietary Allowances. Advance J Food Sci Technol. 2020;2(2):104-108.

Glew, R.S., D.J. VanderJagt R. Bosses, Y.S. Huang L.T. Chuang and R.H. Glew, The nutrient content of three edible plants of the Republic of Niger. J Food Comp Anal. 2005;18: 15-27.

Burkill, H.M. The useful plants of West tropical Africa. London. 1985;1:1-3.

CDC. Sexually Transmitted Disease Surveillance 2019. Atlanta: U.S. Department of Health and Human Services;2021.

Asokan, G. V., & Vanitha, A. WHO global priority pathogens list on antibiotic resistance: an urgent need for action to integrate One Health data. Perspec Pub Health. 2018;38(2):87-88.

CDC Antimicrobial Resistance Coordination and Strategy Unit. Prevention works. Antibiotic Resistance Threats in the United States, 2019, ix. doi:10.15620/cdc:82532

Centers for Disease Control and Prevention (CDC), 2015, Methicillin-resistant Staphylococcus aureus, viewed 08 January 2015, from http://www.cdc.gov/mrsa/

Kamara, MTZ, Huiming, Z, Kexue, I, Amadou and F Tarawalie, (). Comparative Study of chemical Composition and physiochemical properties of two varieties of defatted foxtail. Millet flour grown in China. Am J Food Technol. 2009;4:255-267.

Baorto, EP, Baorto, D, Windle, ML & Lutwick, LI. Staphylococcus aureus infection. WebMD LLC (2014). From http://eMed.medscape.com/article/971358-overview.

Satterwhite, CL, Torrone, E, Meites, E et al. Sexually transmitted infections among US women and men: Prevalence and incidence estimates, 2008. Sex Transm Dis. 2013;40:187-193.

Shimane W. Makhabu, Balisana Marotsi. Changes in herbaceous species composition in the absence of disturbance in a Cenchrus biflorus Roxb. invaded area in Central Kalahari Game Reserve, Botswana. Int J Ecol. 2012;174813, 6 pages, 2012. https://doi.org/10.1155/2012/174813

Bultosa, Geremew, Molapisi, Moenyane, Tselaesele, Nelson, Kobue-Lekalake, Rosemary, Desse Haki, Gulelat, Makhabu, Shimane, Sekwati-Monang, Bonno, Seifu, Eyassu, P. Nthoiwa, Gothatamang. Plant-based traditional foods and beverages of Ramotswa Village, Botswana. J Ethn Food. 2020;7(1): 1-15.

FAO (2003): Food energy - methods of analysis and conversion factors. FAO Food and nutrition paper 77.

AOAC (2000). Official methods of analysis of the association of official analytical chemists, 15th edition, Washington D.C., 20044.

Chen, S, Wu, Q, Shen, Q & Wang, H. Progress in understanding the genetic information and biosynthetic pathways behind Amycolatopsis antibiotics, with implications for the continued discovery of novel drugs. ChemBioChem. 2016;17(2):119-128.

Vollekova, A, Kostalova, D & Sochorova, R. Isoquinoline alkaloid from Mahonia aquifolium stem bark is active against Malsseizia species', Folia Microbiol. 2001;46:107-111.

Jose MC. Diane S. Rapid micro Kjeldahl digestion of cereal grains and other biological material. Anal Biochem. 1973;53(1):35-41.

Oyeleke, A. (1984). Outline of Food Analysis. Ahamadu Bello University Zaria, Nigerian :58.

JL Black and PA Kenney. Factors affecting diet selection by sheep. 2. Height and density of pasture Australian. J Agri Res 1984;35(4):565 - 578.

Yakandawala, D, Morton, CM & Prance, GT. Phylogenetic relationships of the chrysobalanaceae inferred from chloroplast, nuclear and morphological data. Annal Missouri Bot Garden. 2010;97:259-281.

Motlhanka D.M. Makhabu S.W. Medicinal and edible wild fruit plants of Botswana as emerging new crop opportunities. J Med Plant Res. 2011;5(10):1836-1842.

Warra, AA, Umar, RA, Sani, I, Gafar, MK, Nasiru, A & Ado, A. Preliminary phytochemical screening and physicochemical analysis of gingerbread plum (Parinari macrophylla) seed oil. J Pharmacogn Phytochem. 2013;1(2): 20-25.

Dryden, M. Reactive oxygen therapy: A novel therapy in soft tissue infection. Curr Opin Infect Dis. 2017;30(2):143-149.

Aletan U.I. and Kwazo H.A. Analysis of the proximate Composition, (2019) Anti-Nutrients and mineral content of Maerua crassifolia leaves Nigeria. J Basic and Appl Sci. 2019;27(1):89-96.

Magu, T. O., H. Louis, N. Nzeata-Ibe, E. A. Sunday, V. M. Udowo, J. A. Ugur, and I. E. A. Oyo. (). Proximate Analysis and Mineral Composition of Jatropha curcas seeds obtained from Pankshin local government area of Plateau state of Nigeria. J Phys Chem Biophys. 2018;8:265

Kreisel, K.M., Weston, E.J., St. Cyr, S.B., Spicknall, I.H.Estimates of the Prevalence and Incidence of Chlamydia and Gonorrhea Among US Men and Women, 2018. Sex Transm Dis. 2021;1;48(4):222-231.

Akomal O.A., Nmal N. Y,. Musa S.A and Salihu A.B. Nutritional and Phytochemical composition of Vitellaria paradoxa (Shea fruit pulp). Int J Biochem Res Rev. 2018;22(1):1-7

Aliyu Muhammad, Sayudi Yahaya Haruna, Abubarkar Umar Birnin Yauri, Aminu Haruna Muhammad, Como's Moki Elinge. Nutritional and Anti-Nutrientional Composition of ximenia Americana Fruit. Am J Appl Chem. 2019;7:123-129.

Yinusa, I, George, NI, Shuaibu, OA & Ayo, RG. Bioactivity of stigmasterol isolated from the aerial part of Spillanthes acmella (Murr) on selected microorganism. Int J Curr Microbiol Appl. 2014;3(2):475-479.

Fagbohun, E.D., Lawal, O.U. and Ore, M.E. The proximate, mineral and phytochemical analysis of the leaves of Ocimum gratissimum L., Melanthera scandens A. and Leea guineensis L. and their medicinal value. Int J Appl Biol Pharma Technol. 2012;3(1):15-22.

Olubukola O. Ruthven L. A navigational guide for refugee integration. Info Process Manage. 2019;56(3):791-808.

Green, BN, Johnson, CD, Egan, JT, Rosenthal, M, Griffith, EA & Evans, MW. Methicillin-resistant Staphylococcus aureus: An overview for manual therapists. J Chiropractic Med. 2012;11(1):64-76.

Ochoa, JT, Barletta, F, Contreras, C & Mercado, E. New insights into the epidemiology of enteropathogenic Escherichia coli infection. Trans. R. Soc. Trop. Med. Hyg. 2008;102(9):852-856.

Muhammad, S., Umar, k.J., Sani, N.A. Evaluation of Nutritional and Anti-Nutrientional profiles of Gingerbread plum (Neocarya macrophylla) Seed Kernel from Sokoto State, Nigeria. Int J Sci Technol. 2015;4(7):361-367.

Weiner-Lastinger, LM, Pattabiraman, V, Konnor, RY, Patel, PR, Wong, E, Xu, SY, Smith, B, Edwards, JR, & Dudeck, MA. The impact of coronavirus disease 2019 (COVID-19) on healthcare-associated infections in 2020: A summary of data reported to the National Healthcare Safety Network. Infect Control Hosp Epidemiol. 2022;43(1):12-25.

Pawar, HA. Natural product as a source of lead to the design of new drugs. Nat Product Chem Res. 2014;2:156.

Siddiqui, AA, Iram, F, Siddiqui, S & Sahu, K. Role of natural products in drug discovery process. Int J Drug Develop Res. 2014;6(2):0-0.

Ajifolokun OM, Adeniran HA. Proximate and Mineral Composition of Co-Fermented Breadfruit and Cassava into Gari Analogue. J Nutr Food Sci. 2018;8:658.

Hassan L.G, Abdulmin U, UmarK.K Ikeh P.O, and Aliero A.A. Nutritional and anti- nutritional composition of Strychnos innocua Del. ( Monkey orange) fruit pulp of Africa locust bean (Parkia biglobosa L.). Niger J Basic Appl Sci. 2014;13:15-27.

Tamokou, JD, Kuiate, JR, Tene, M, Nwemeguela, TJK & Tane, P. The antimicrobial activities of extract and compounds isolated from Brillantais lamium. Iran J Med Sci. 2011;36(1):24-31.

Ayrton, F, A Júlia, S Daiane, F Jaqueline, M Darliana. Determination of statin drugs in hospital effluent with dispersive liquid-liquid microextraction and quantification by liquid chromatography. J Environ Sci Heal A. 2017;52(10):1-7.

Aziz S, Saha k, Sultana N, But HP, Ahsan MA, Ahmed S, Hossain M. Comparative studies of elemental composition in leaves and flowers of Catharanthus roseus growing in Bangladesh. Asia Pac J Trop Biomed. 2016;6(1):50-54.

World Health Organization (WHO), 2002, Use of antimicrobials outside human Med and resultant antimicrobial resistance in humans, Fact sheet 268, WHO, Geneva.

Khan L. Development of an indigenous screening instrument in Pakistan: the Aga Khan University Anxiety and Depression Scale. J Pak Med Assoc. 1998;8(9):261.

Sunday EA, Israel AU, Magu T.O. Proximate analysis and mineral element Composition of false yam (icacina trichantha). tuber and oyster mushroom (Pleurotus ostreatus). Int J Chem Sci. 2016;1:31-39.

Akakuru OU, Magu TO, Louis H, Maitera ON, Fidelis TT. Comparative study of some heavy metal concentrations in coconut fluid and milk obtained from Taraba, Imo and Adamawa states, Nigeria. Int J Sci Res Pub. 2017;7:138-140.

Yusuf, AJ, Abdullahi, MI, Haruna, AK, Idris, AY & Musa, AM. Isolation and characterization of stigmasterol and Bis-(5,7-diacetyl-catechin-4-?-rhamnopyranoside) from the stem bark of Neocarya macrophylla (Sabine) prance (Chrysobalanaceae)', Niger J Basic Appl Sci. 2015;23(1):15-22.

Downloads

Published

31.07.2022

How to Cite

Garba, J. ., Oba, A., Ofili, A. ., John, B., Isah, H., John, K. V., & Musa, J. A. . (2022). Phytochemical Screening, Proximate Composition and Mineral Element Analysis of Neocarya macrophylla (Gingerbread) Plum and its Effects on Microorganisms. Journal of Biochemistry, Microbiology and Biotechnology, 10(1), 76–81. https://doi.org/10.54987/jobimb.v10i1.716

Issue

Section

Articles