Isolation and Screening of Biosurfactant-producing Bacteria from Hydrocarbon-contaminated Soil in Kano Metropolis, Nigeria

Authors

  • Aminu Yusuf Fardami Department of Microbiology, Usmanu Danfodiyo University, 840104, Sokoto, Nigeria.
  • Abdullahi Hassan Kawo Department of Microbiology, Faculty of Basic Medical Sciences, College of Health Science, Bayero University Kano, Kano, PMB 3011, Nigeria.
  • Sani Yahaya Department of Microbiology, Faculty of Basic Medical Sciences, College of Health Science, Bayero University Kano, Kano, PMB 3011, Nigeria.
  • Maryam Lami Riskuwa-Shehu Department of Microbiology, Usmanu Danfodiyo University, 840104, Sokoto, Nigeria.
  • Ibrahim Lawal Department of Biological Sciences, Al-Qalam University Katsina, Dutsinma Road, 820102, Katsina State, Nigeria.
  • Haruna Yahaya Ismail Department of Microbiology, University of Maiduguri, Borno State, P.M.B 1069 Maiduguri, Nigeria.

DOI:

https://doi.org/10.54987/jobimb.v10i1.664

Keywords:

Biosurfactant, Screening, Hydrocarbon, Contaminated Soil, Bacteria

Abstract

Biosurfactants are surface-active biomolecules produced by microorganisms that have different applications in solving many environmental problems. This study was carried out to screen biosurfactant-producing bacteria isolated from hydrocarbon-contaminated soil of Kano Metropolis, Kano State- Nigeria. Soil samples were collected and processed. Biosurfactant-producing bacteria were enumerated, isolated and characterized using cultural, morphological and biochemical characteristics. Blood haemolysis, oil drop collapse and oil displacement tests were employed for the screening of the bacterial isolates for the potential to produce biosurfactant. The viable aerobic heterotrophic bacterial count of the samples ranges from 1.0 to 8.4×106 cfu/g. Eight bacterial genera were biochemically identified as Pseudomonas aeruginosa, Enterobacter cloacae, Pantoea agglomerans, Pseudomonas sp., Bacillus subtilis, Enterobacter alvei, Bacillus sp. and Klebsiella sp. Bacillus subtilis had the highest frequency of occurrence of 5(27%) while Bacillus sp. and Enterobacter alvei have the least occurrences of 1(6%) each. The eight identified bacterial isolates were all positive for the haemolysis test, drop collapse and oil displacement test.

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Published

31.07.2022

How to Cite

Fardami, A. Y. ., Kawo, A. H., Yahaya, S., Riskuwa-Shehu, M. L. ., Lawal, I. ., & Ismail, H. Y. (2022). Isolation and Screening of Biosurfactant-producing Bacteria from Hydrocarbon-contaminated Soil in Kano Metropolis, Nigeria. Journal of Biochemistry, Microbiology and Biotechnology, 10(1), 52–57. https://doi.org/10.54987/jobimb.v10i1.664

Issue

Vol. 10 No. 1 (2022)

Section

Articles

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