Study of Enzymatic Activity of Fungi Isolated from Spoiled Bread Found in Contaminated Soil Around Birnin Kebbi City, Kebbi state Nigeria

Authors

  • Jamilu Garba Independent Researcher
  • Asime Oba Independent Researcher
  • David N. BukBuk Department of Microbiology, University of Maiduguri, P.M.B 1069 Maiduguri, Borno State, Nigeria.
  • Barka John eterinary teaching hospital, Faculty of Veterinary Medicine, University of Maiduguri, P.M.B 1069 Maiduguri, Borno State, Nigeria.
  • Ibrahim Alkali Allamin epartment of Microbiology, University of Maiduguri, P.M.B 1069 Maiduguri, Borno State, Nigeria.
  • Hadiza Isah Independent researcher, P.M.B 1069 Maiduguri, Borno State, Nigeria.
  • Kwata Veronica John Department of Veterinary pathology, Faculty of Veterinary Medicine, University of Maiduguri, P.M.B 1069 Maiduguri, Borno State, Nigeria.
  • Jasini Athanda Musa Department of Public Health, Faculty of Veterinary Medicine, University of Maiduguri, P.M.B 1069 Maiduguri, Borno State, Nigeria.

DOI:

https://doi.org/10.54987/jobimb.v10i1.662

Keywords:

Bread, Enzymes, Aspergillus, Penicillium, Rhizopus

Abstract

enzymes ranging from biochemical substances that are of industrial significance that includes by-products of both primary and secondary metabolites. Ethanol, citric acid, gluconic acid, itaconic acid, amino acid, Vitamins, nucleotides and polysaccharides provide examples of primary metabolites produced by fungi. Antibiotics such as penicillin, cephalosporin, fusidic acid and griseofulvin are important secondary metabolites. Fungi are well known as a source of antibiotics but new therapeutic compounds with novel pharmacological activities have been developed in recent years. Loaves of spoilt Bread recruited for this study were collected from different Areas of Birnin Kebbi town and exposed for 7 days in different Areas and are assessed daily. Potato Dextrose Agar (PDA) was used as a culture medium for the isolation. Three fungal species were isolated from Bread samples from contaminated sites using Potato Dextrose Agar. Characterization was based on classical microscopic and macroscopic techniques of color margin and elevation of pure colonies. The consumption of the spores of Fungi that is associated with the spoilage of Bread leads to an increase in Airborne yeasts infections, previous studies had shown that most fungi species that grow on bread stand a major factor of respiratory infection due to their cosmopolitans in the distribution of spores.

References

Unachukwu, M. N and Nwakanma, C. The fungi associated with the spoilage of bread in Enugu state. Int J Curr Microbiol App Sci. 2018;4(1):989-995.

Nirmmala, R. Pathamanathan, S. Selvaratnam, S. Int J Adv Biol Sci. 2016;3(4):165-167.

Brian, F. Air sampling for fungi in indoor environments. Int J Aero Sci. 1997;28(3):381-392.

Thakre J, N. V. and Kolet, M. Preservatives used for control of fungal spoilage of bread. Int J Life Sci. Special Issue A9, 2018;9:2018. UGC Approved Journal No 4895.

Berni E and Scaramuzza N. Effect of ethanol on growth of Chrysonilia sitophila ('the red bread mould') and Hyphopichia burtonii ('the chalky mould') in sliced bread. Lett Appl Microbiol. 2013;57(4): 344-349.

Caio GO, Pontes SFO, Medeiros EAA and Soares NFF. Edible films from methylcellulose and nanoemulsions of clove bud (Syzygium aromaticum) and Oregano (Origanum vulgare) essential oils as shelf-life extenders for sliced bread. J Agric Food Chem. 2014;62(22):5214-5219.

Nielsen PV and Rios R. Inhibition of fungal growth on bread by volatile components from spices and herbs, and the possible application in active packaging, with special emphasis on mustard essential oil. Int J Food Microbiol. 2000;60(2-3):219-229.

Lotfinia S, Javanmard, DM and Mohammadi NA. Application of Starch Foams Containing Plant Essential Oils to Prevent Mold Growth and Improve Shelf Life of Packaged Bread. J Chem Health Risk. 2013;3(4):9-18.

Lakins DG, Echeverry A, Alvarado CZ, Brooks JC, Brashears MT and Brashears MM. Quality of mold growth on white enriched bread for military rations following directional microwave treatment. J Food Sci. 2008;73(3):99-103.

Axel C, Zannini E and Arendt EK. Mold spoilage of bread and its biopreservation: A review of Current strategies for bread shelf life extension, Crit Rev Food Sci. Nutr. 2017;57(16):3528-3542.

Unachukwu MN and Nwakanma C. The fungi associated with the spoilage of bread in Enugu state. Int J Curr Microbiol App Sci. 2015;4(1):989-995.

Jesen, P.R. and Fenical., W. Marine microorganisms and drug discovery Curr status and future potential. In . Fusetani., N. (eds). Drugs from the sea. Basel.. karger. Microbiol. 2000

Borel, J.F.History of the discovery of cyclosporine and of its early pharmacological development. J Biotechnol. 2002;114:433-437.

Vazhacharikal PJ, Mathew JJ, Sajeshkumar NK and Prathap P. Effect of concentration and pH on the preservative action of calcium propionate against black bread mold (Rhizopus stolonifer) in Kerala. CIB Tech J Biotechnol. 2015;4(2):1-12.

Ryan LA, Dal Bello F and Arendt EK. The use of sourdough fermented by antifungal LAB to reduce the amount of calcium propionate in bread. Int J Food Microbiol. 2008;125(3):274-278.

Baker, KL; Beneke, ES; Hooper, GR; Fields, WG. Host range and axenic culture of the mycoparasite Syncephalis sphaerica (Mucorales). Mycologia. 1977;69(5):1008-1015.

Benny, G.L., Smith, M.E., Kirk, p.m., Tretter, E.D, White, M.M. Challenges and Future Perspectives in the Systematics of Kickxellomycotina, Mortierellomycotina, Mucoromycotina, and Zoopagomycotina". Biology of Microfungi pp 65-126 ;2016

Shahnawaz M, Lohano DK and Sheikh SA. A study on the impact of chemical preservatives on sensorial excellence of bread at various temperatures. Int. J. Res. in Ayurveda and Pharmacy, 2012;3(6):833-836.

Marcelo,V. G. Curr Opinion in Food Science Volume 29, October 2019, Pages 1-6.Department of Technology and Food Science, Center of Rural Sci, Federal University of Santa Maria (UFSM), Postal 97105-900, Santa Maria, RS, Brazil.

Ayerst, G.. The effects of moisture and temperature on growth and spore germination in some fungi Department of App Science, Wolverhampton College of Technology, Wolverhampton, England. J. St. Prod. Res. Volume 5, Issue 2, August 1969, pp 127-141.

Wang J, Pawelzik E, Weinert J, Zhao Q and Wolf GA. Effect of fungicide treatment on the quality of wheat flour and bread making. J Agric Food Chem. 2004;52(25):7593-7600.

Berni E and Scaramuzza N. Effect of ethanol on growth of Chrysonilia sitophila ('the red bread mould') and Hyphopichia burtonii ('the chalky mould') in sliced bread. Lett. Appl. Microbiol., 2013;57(4):344-349.

Ravimannan N, Sevvel P and Saarutharshan S. Study on fungi associated with spoilage of bread. Int J Adv Res Biol. Sci., 2016;3(4):165-167.

Tang, Q., Bie, X., Lu, Z., Lv, F., Tao, Y., Qu, X. Effects of Fengycin from Bacillus subtilis fmbJ on apoptosis and necrosis in Rhizopus stolonifer. J Microbiol. 2014;52:675-680.

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Published

31.07.2022

How to Cite

Garba, J., Oba, A., BukBuk, D. N. ., John, B., Allamin, I. A. ., Isah, H., John, K. V. ., & Musa, J. A. . (2022). Study of Enzymatic Activity of Fungi Isolated from Spoiled Bread Found in Contaminated Soil Around Birnin Kebbi City, Kebbi state Nigeria . Journal of Biochemistry, Microbiology and Biotechnology, 10(1), 41–45. https://doi.org/10.54987/jobimb.v10i1.662

Issue

Vol. 10 No. 1 (2022)

Section

Articles

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