Antibacterial Effect of Bitter Leave (Vernonia amygdalina) on Klebsiella pneumoniae

Authors

  • Haruna Sa’idu Department of Biology, Faculty of Sciences, Gombe State University, P.M.B 127, Tudun Wada, Gombe, Gombe State, Nigeria.
  • Hassan Ismail Ahmad Department of Biology, Faculty of Sciences, Gombe State University, P.M.B 127, Tudun Wada, Gombe, Gombe State, Nigeria.
  • Salau Abiola Olanrewaju Department of Natural Sciences, Gombe State College of Education,Billiri, Gombe State, Nigeria.
  • Aisha Bello Mahmoud National Biotechnology Development Agency, Nigeria.

DOI:

https://doi.org/10.54987/jobimb.v8i2.538

Keywords:

Antibacterial; bitter leaf; medical; multi drug resistance

Abstract

High rate of multi drug resistance bacteria caused by Klebsiella pneumoniae is increasing day by day, which in turn becomes medical and economic important to humans. The present study investigates the antibacterial effect of bitter leaf on Klebsiella pneumoniae. Frothing test and agar well diffusion method was used in this study. The former method indicate the presence of phytochemicals such saponin, tannin, flavonoid, alkaloid and carbohydrate in the plant while the later method indicate the potential effect of the plant extract to inhibit the growth of Klebsiella pneumoniae under different concentration ranging 2.5, 5, 10 20 mg/mL. The various zones of inhibition obtained under these concentrations were 0.0±0, 7±0.8, 11±0.6 and 14.00±1.3 mm, respectively. The highest zone of inhibition recorded was 26.00±2.3 mm using control experiment whereby no activity was obtained using 2.5 mg/mL plant extract. Thus, increase in the concentrations of extract was found to inhibit the growth of the bacteria under study. This research reports the potential effect of Vernonia amygdalina on the activity of K. pneumoniae.

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Published

28.12.2020

How to Cite

Sa’idu, H., Ahmad, H. I., Olanrewaju, S. A., & Mahmoud, A. B. (2020). Antibacterial Effect of Bitter Leave (Vernonia amygdalina) on Klebsiella pneumoniae. Journal of Biochemistry, Microbiology and Biotechnology, 8(2), 16–20. https://doi.org/10.54987/jobimb.v8i2.538

Issue

Vol. 8 No. 2 (2020)

Section

Articles

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