Electroduction on Azospirillum brasilense Sp7 and Chromobacterium violaceum

Authors

  • Wai Keong Loke Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
  • Halimi Mohd Saud Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.

DOI:

https://doi.org/10.54987/jobimb.v8i2.537

Keywords:

Electroduction; Azospirillum brasilense Sp7; Chromobacterium violaceum; cosmid vector pLAFR1

Abstract

Electroduction is a fast, simple and very efficient transformation method with a wider range of bacteria strains which involved physical process by exposing to high electricity. A successful transfer of a 21.6 kb cosmid vector pLAFR1 into Azospirillum brasilense Sp7 and Chromobacterium violaceum was achieved with the maximum efficiency of about 35 X 102 and 24 X 102 transformants, respectively, at the electrical field strength of 5kV/cm with longer pulse length range between 8.3 and 8.5 milliseconds. Doubling the pulse length and a decrease or increase in the electrical field strength of 5kV/cm resulted in a reduction in the total number of transformants. The electroduction parameters were very important to make this transformation method works efficiently.

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Published

28.12.2020

How to Cite

Loke, W. K., & Saud, H. M. (2020). Electroduction on Azospirillum brasilense Sp7 and Chromobacterium violaceum. Journal of Biochemistry, Microbiology and Biotechnology, 8(2), 13–15. https://doi.org/10.54987/jobimb.v8i2.537

Issue

Vol. 8 No. 2 (2020)

Section

Articles

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