Multiple Drug Resistance Among Staphylococcus aureus Strains Isolated from Cutting Boards of Commercial Food Premises: A Threat to Food and Public Health Safety

Authors

  • Nor Ainy Mahyudin Department of Food Service and Management, Faculty of Food Science and Technology, Universiti Putra Malaysia, Malaysia.
  • Shakira Mohd Sahil Department of Food Service and Management, Faculty of Food Science and Technology, Universiti Putra Malaysia, Malaysia.
  • Son Radu Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Malaysia.
  • Nor Khaizura Mahmud @Ab Rashid Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Malaysia.
  • Shu’aibu Isa Department of Microbiology, Gombe State University, Gombe, Gombe State Nigeria.

DOI:

https://doi.org/10.54987/jobimb.v7i1.454

Keywords:

Staphylococcus aureus; multidrug resistance; antibiotics; cutting boards; restaurants

Abstract

Multidrug resistance among bacterial pathogens is an issue of global concern, especially in commercial food premises. This study aimed to isolate and identify the multidrug-resistant S. aureus from cutting boards of some selected restaurants in Sri Serdang, Selangor, Malaysia. Samples were obtained from the surfaces of 55 plastic cutting boards of 45 restaurants by swabbing and analyzed using 3Mâ„¢ Petri filmâ„¢ Staph Express Count Plate and Disk and further identified using Gram staining, coagulase and catalase tests and growth on mannitol salt agar and Baird Parker agar. Agar-disk diffusion technique was employed for antibiotic resistance against 11 antibiotics, and the results were analyzed based on CLSI. Out of 55 samples obtained, 43.6% (n=24) were found to be positive for S. aureus. Of the 24 S. aureus, 45.8% (n=11) were multidrug-resistant. Resistance to the members of penicillins was 100%, to nalidixic acid (79.2%), to ciprofloxacin (66.7%), to cephalothin (33.3%) and ceftazidime (20.8%). There was no resistance to gentamicin, streptomycin, ceftriaxone, cefotaxime, and sulphafurazole. The emergence of multidrug-resistant S. aureus on the cutting boards is an indication of poor personal and sanitary hygiene and could pose a danger of outbreak due to staphylococcal food poisoning among consumers.

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Published

31.07.2019

How to Cite

Mahyudin, N. A., Mohd Sahil, S., Radu, S., Mahmud @Ab Rashid, N. K., & Isa, S. (2019). Multiple Drug Resistance Among Staphylococcus aureus Strains Isolated from Cutting Boards of Commercial Food Premises: A Threat to Food and Public Health Safety. Journal of Biochemistry, Microbiology and Biotechnology, 7(1), 48–51. https://doi.org/10.54987/jobimb.v7i1.454

Issue

Vol. 7 No. 1 (2019)

Section

Articles

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