Encapsulation of Lactobacillus plantarum with Mannan and Sodium Alginate Improves its Cell Production
DOI:
https://doi.org/10.54987/jobimb.v7i1.447Keywords:
Encapsulation; Lactobacillus plantarum; mannan; oligosaccharides; Response Surface MethodologyAbstract
Probiotic bacteria are utilized in aquaculture as they exert a crucial function in promoting and maintaining the fish health. Probiotic strains should be present in a viable form during consumption and throughout the gastrointestinal tract for maximum health benefits. Many reports stated that there is poor survival of probiotic in products containing free probiotic cells. Providing probiotic living cells with physical barrier to resist adverse environmental conditions is therefore an approach currently receiving considerable interest. In this study, Lactobacillus plantarum was encapsulated with mannan and sodium alginate to increase probiotic viability. Response surface methodology (RSM) based on Box-Behnken design was used to optimize the encapsulation process with mannan concentration (5 to 30 % w/v) and sodium alginate concentration (1 to 5 % w/v) as the independent variables evaluated. According to the regression coefficients and significance of the polynomial model, the optimum encapsulation parameters were as follows: 24.73 % w/v mannan; 1.6 % w/v sodium alginate. Under these conditions of encapsulation, the total cell production of the Lactobacillus plantarum was increased to 5.3 (108 CFU/g) as compared to the free cell culture, 3.2 (108 CFU/g).
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).