Characterization of COOH-Fe<sub>3</sub>O<sub>4</sub>/NCC-CTA<sup>+</sup> on screen printed carbon electrode using Field Emission Scanning Electron Microscope and Energy Dispersive X-Ray for DNA biosensor

Authors

  • Che Engku Noramalina Che-Engku-Chik Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43300 Serdang, Selangor Darul Ehsan, Malaysia.
  • Nor Azah Yusof Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43300 Serdang, Selangor Darul Ehsan, Malaysia.
  • Jaafar Abdullah Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43300 Serdang, Selangor Darul Ehsan, Malaysia.
  • Siti Sarah Othman Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43300 Serdang, Selangor Darul Ehsan, Malaysia.
  • Helmi Wasoh Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43300 Serdang, Selangor Darul Ehsan, Malaysia.

DOI:

https://doi.org/10.54987/jobimb.v5i2.343

Keywords:

biosensor; DNA; nanocomposite; nanocellulose crystalline; screen printed carbon electrode

Abstract

A novel DNA biosensing platform was designed by the functionalization of iron oxide (Fe3O4)
with the carboxylic group via capping agent, mercaptopropionic acid (MPA) and conjugated
with nanocellulose crystalline (NCC) surface modified with surfactant cetyltrimethylammonium
bromide (CTAB) to assist in the DNA sensing capability. The product of nanocomposites
compound was drop-casted on screen printed carbon electrode (SPCE). Characterization by field
emission scanning electron microscope (FESEM) and energy dispersive X-Ray (EDX)
spectroscopy showing that carboxyl functionalized iron oxide (COOH-Fe3O4) can be hybridized
with NCC-CTA+ via electrostatic interaction.

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Published

31.12.2017

How to Cite

Che-Engku-Chik, C. E. N., Yusof, N. A., Abdullah, J., Othman, S. S., & Wasoh, H. (2017). Characterization of COOH-Fe<sub>3</sub>O<sub>4</sub>/NCC-CTA<sup>+</sup> on screen printed carbon electrode using Field Emission Scanning Electron Microscope and Energy Dispersive X-Ray for DNA biosensor. Journal of Biochemistry, Microbiology and Biotechnology, 5(2), 19–21. https://doi.org/10.54987/jobimb.v5i2.343

Issue

Vol. 5 No. 2 (2017)

Section

Articles

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