Isolation of molybdenum-reducing bacterium; <i>Serratia</i> sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation

Authors

  • Mohd Izuan Effendi Halmi Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43600 UPM Serdang, Selangor, Malaysia.
  • Wan Lutfi Wan Johari Department of Environment, Faculty of Environmental Studies, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Mohd Shukuri Mohd Ali Department of Environment, Faculty of Environmental Studies, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Noor Azmi Shaharuddin Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. 3Department of Environment, Faculty of Environmental Studies, Universiti Putra

DOI:

https://doi.org/10.54987/jobimb.v5i2.342

Keywords:

Bioremediation; molybdenum blue; agriculture soil; isolation; Serratia sp.

Abstract

Molybdenum is reported to be very toxic to ruminants and shows evidence of spermatogenesis
toxicity in animals and insects. Hence, its removal is important. In this study, we report on the
first isolation of molybdenum-reducing bacterium from agricultural soil. The bacterium reduces
hexavalent molybdenum (sodium molybdate) to molybdenum blue (Mo-blue); a colloidal
product, which can be trapped and removed from solution. Phylogenetic analysis resulted in a
tentative identification of the bacterium as Serratia sp. strain MIE2. The optimum conditions for
Mo-blue production using the normal one-variable-at-a-time (OVAT) approach were 10 mM of
sodium molybdate, pH 6.0, a temperature of 35°C, ammonium sulphate at 10 g/L as the nitrogen
source and sucrose concentrations of between 30-50 g/L as the carbon source and electron donor
for molybdate. Studies on the effects of pesticides and solvents on Mo-blue production showed
that Mo-blue production from whole cells was relatively more affected by these xenobiotics
compared to the crude enzyme. Nevertheless, the strain was resistant to most of the xenobiotics
tested. Based on the strain MIE characteristics, the bacterium will be a suitable candidate for the
remediation of aquatic bodies and agricultural soils contaminated with molybdenum.

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Published

31.12.2017

How to Cite

Halmi, M. I. E., Johari, W. L. W., Ali, M. S. M., & Shaharuddin, N. A. (2017). Isolation of molybdenum-reducing bacterium; <i>Serratia</i> sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation. Journal of Biochemistry, Microbiology and Biotechnology, 5(2), 12–18. https://doi.org/10.54987/jobimb.v5i2.342

Issue

Vol. 5 No. 2 (2017)

Section

Articles

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