Expression of recombinant E1 Glycoprotein of Chikungunya virus in Baculovirus expression system

Authors

  • Magdline Sia Henry Sum Department of Biochemistry UPM
  • Anna Andrew
  • Milda Aren Maling

DOI:

https://doi.org/10.54987/jobimb.v3i1.232

Abstract

Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, the envelope E1 gene of CHIKV was cloned and expressed in a baculovirus system. The recombinant E1 protein with N-term 6-His residues protein was successfully expressed and purified as confirmed by SDS-PAGE and western blot analysis. The seroreactivity of the recombinant protein was evaluated in immunoassay for anti-CHIKV IgM and IgG antibodies. The recombinant antigen showed 69% sensitivity and 100% specificity for anti-CHIKV IgG by dot blot assay. Detection of anti-CHIKV IgM by dot assay showed 79% sensitivity and 100% specificity. No cross reactivity of the antigen was observed with anti-dengue virus serum samples. The results strongly support that the recombinant E1 protein has potential to be used as diagnostic antigen. The used of the antigen in a dot blot assay gives an advantage for laboratory detection without the need of any specialised equipment.

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Published

30.10.2015

How to Cite

Henry Sum, M. S., Andrew, A., & Maling, M. A. (2015). Expression of recombinant E1 Glycoprotein of Chikungunya virus in Baculovirus expression system. Journal of Biochemistry, Microbiology and Biotechnology, 3(1), 26–29. https://doi.org/10.54987/jobimb.v3i1.232

Issue

Vol. 3 No. 1 (2015)

Section

Articles

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