Optimization of monoclonal antibodies purification expressed in H-192 cells using preparative native-polyacrylamide gel electrophoresis

Authors

  • Santhana Krishnan Department of Biochemistry UPM
  • Zularisam Abdul Wahid
  • Chua Yeo Gek Kee
  • Chew Few Nee
  • Mimi Sakinah Abdul Munaim

DOI:

https://doi.org/10.54987/jobimb.v3i1.231

Abstract

Monoclonal antibodies (mAbs) are unique and specific drug molecules targeting the treatment of various diseases such as arthritis, immune disorders, infectious diseases, and cancer etc. Different methods such as antibody coupled affinity chromatography, hydrophobic interaction chromatography, etc., can be applied to purify mAbs from various sources. This article provides a simple, cost effective, preparative native-polyacrylamide gel electrophoresis (n-PAGE)
technique to purify mAbs expressed in H-192 cells (Hybridoma murine cell lines) against an antigen i.e. 17-alpha-hydroxyprogesterone (17-OHP), which further can have diagnostic application to detect Congenital Adrenal Hyperplasia (CAH). Furthermore, different parameters such as concentration and volume of the feedstock (medium containing antibodies), pore size of gel, height of resolving gel etc. were optimized to obtain the maximum purity and yield of mAbs.

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Published

30.10.2015

How to Cite

Krishnan, S., Abdul Wahid, Z., Gek Kee, C. Y., Few Nee, C., & Abdul Munaim, M. S. (2015). Optimization of monoclonal antibodies purification expressed in H-192 cells using preparative native-polyacrylamide gel electrophoresis. Journal of Biochemistry, Microbiology and Biotechnology, 3(1), 21–25. https://doi.org/10.54987/jobimb.v3i1.231

Issue

Vol. 3 No. 1 (2015)

Section

Articles

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