Identification of methane-producing bacteria from palm oil mill sludge (POMS) with solid cud from ruminant stomach

Authors

  • Chan, C. S. W Univerisiti Putra Malaysia
  • S. Lau Department of Chemistry, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak
  • A.A. S. Husaini Department of Chemistry, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak
  • A. Zulkharnain Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 943000 Kota Samarahan, Sarawak
  • K. Apun Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 943000 Kota Samarahan, Sarawak
  • L. M. Bilung Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 943000 Kota Samarahan, Sarawak
  • Mickey Vincent Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 943000 Kota Samarahan, Sarawak

DOI:

https://doi.org/10.54987/jobimb.v2i1.125

Abstract

Biological generation in anaerobic environments such as enteric fermentation and anaerobic waste treatment from agriculture sector are the major contributor of methane gas which has the potential as biogas. The aimed of this study was to identify methane-producing bacteria in anaerobic vessel which contained a mixture of Palm Oil Mill Sludge (POMS) and solid cud taken from the first compartment of cow’s stomach (1:2 and 2:1 ratio) as co-mixture. The co-mixture was incubated at 50 °C in a 2 L vessel with initial starter of 400 ml and sampling was conducted every 4 weeks interval during 12 weeks of incubation. For specific detection of methanogens, 16S rRNA-cloning analysis was carried out. Methanobrevibacter sp. and Methanosaeta sp. were confirmed to be presence within the 2:1 ratio of co-mixture while only Methanobrevibacter sp. was found in 1:2 ratio of co-mixture on both Week 0 and Week 4. No methanogens were detected for both co-mixtures on Week 8 and Week 12.  

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Published

31.07.2014

How to Cite

C. S. W, C., Lau, S., Husaini, A. S., Zulkharnain, A., Apun, K., Bilung, L. M., & Vincent, M. (2014). Identification of methane-producing bacteria from palm oil mill sludge (POMS) with solid cud from ruminant stomach. Journal of Biochemistry, Microbiology and Biotechnology, 2(1), 23–26. https://doi.org/10.54987/jobimb.v2i1.125

Issue

Vol. 2 No. 1 (2014)

Section

Articles

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