Molecular approach for identification of fungi from soils of Cameron Highlands

Authors

  • Shamala Salvamani Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Baskaran Gunasekaran Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Norazah Mohammad Nawawi Faculty of Biotechnology and Life sciences, Universiti Selangor, 45 600, Bestari Jaya, Selangor, Malaysia.

DOI:

https://doi.org/10.54987/jobimb.v2i1.123

Abstract

Seven cultures of fungi were isolated from the soil of Cameroon Highlands. In this research, molecular approach was used for the identification of these fungi. The genomic DNA of the fungi species was extracted and the target sequences within the DNA were amplified using polymerase chain reaction (PCR) with ITS 1 (forward) and ITS 4 (reverse) as the primers which amplified the Internal Transcribe Spacer (ITS) of the fungi. The size of PCR product was 600 base pairs. The PCR products were purified and the DNA purity obtained for culture 1, 2, 3 and 4 were 1.74, 1.92, 1.83 and 1.77, respectively. Based on the sequences of the PCR products, three species were successfully identified as Aspergillus flavus, Aspergillus niger and Fusarium oxysporum. 

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Published

31.07.2014

How to Cite

Salvamani, S., Gunasekaran, B., & Nawawi, N. M. (2014). Molecular approach for identification of fungi from soils of Cameron Highlands. Journal of Biochemistry, Microbiology and Biotechnology, 2(1), 7–16. https://doi.org/10.54987/jobimb.v2i1.123

Issue

Vol. 2 No. 1 (2014)

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Articles

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