Characterisation of Salmonella enterica serovar Typhimurium Bacteriophage vB_SenS_ST10: Host-Range Determination, Efficiency of Plating, and Antibiofilm Activity
DOI:
https://doi.org/10.54987/jobimb.v13i1.1074Keywords:
Antimicrobial resistance, Poultry farming, Bacterial control, Viral therapy, Food safetyAbstract
The Malaysian poultry industry, a significant contributor to the agricultural sector and national GDP, is increasingly challenged by antibiotic-resistant bacterial infections, particularly from Salmonella enterica serovar Typhimurium. The widespread use of antibiotics in poultry farming has driven the emergence of resistant strains, highlighting the need for alternative antimicrobial strategies. This study evaluated the characteristics and therapeutic potential of bacteriophage vB_SenS_ST10 as a biological control agent against Salmonella infections in poultry. Host range analysis revealed that vB_SenS_ST10 exhibited a narrow host range, effectively infecting only three of the thirty-two bacterial isolates tested: S. Typhimurium 8720/06, S. enterica (SCC), and S. Tennessee. Efficiency of plating (EOP) analysis indicated reduced binding efficiency for S. enterica (SCC) with an EOP value of 1.5 x 10⁻² relative to the reference strain. Biofilm inhibition assays demonstrated significant (P < 0.05) biofilm suppression at phage concentrations above 10⁴ PFU/mL, though a plateau was observed at higher levels, and complete biofilm eradication was not significantly achieved even at 10⁹ PFU/mL. Importantly, vB_SenS_ST10 did not affect beneficial gut bacteria, such as Lactobacillus and Bifidobacterium species, supporting its potential for targeted antimicrobial application without disrupting gut microbiota. The selective nature of vB_SenS_ST10, combined with its ability to inhibit biofilm formation, presents a promising approach to mitigate Salmonella-associated contamination in poultry production. However, further research is necessary to optimise its application and investigate mechanisms underlying biofilm resilience in poultry production systems.
References
Statista. Per capita poultry consumption in Malaysia from 2006 to 2025. 2023 [cited 2025 Aug 13]. Available from: https://www.statista.com/statistics/757983/malaysia-poultry-consumption-per-capita/
Dar MA, Ahmad SM, Bhat SA, Ahmed R, Urwat U, Mumtaz PT, et al. Salmonella typhimurium in poultry: a review. Worlds Poult Sci J. 2017;73(2):345-54.
Milanov D, Ljubojevi? Peli? D, Cabarkapa I, Karabasil N, Velhner M. Biofilm as risk factor for Salmonella contamination in various stages of poultry production. Biotechnol Anim Husb. 2017;33(1):81-92.
Harrell JE, Hahn MM, D'Souza SJ, Vasicek EM, Sandala JL, Gunn JS, et al. Salmonella biofilm formation, chronic infection, and immunity within the intestine and hepatobiliary tract. Front Cell Infect Microbiol. 2021;10:615221.
Shaji S, Selvaraj RK, Shanmugasundaram R. Salmonella infection in poultry: a review on the pathogen and control strategies. Microorganisms. 2023;11(11):2814.
Osman AY, Elmi SA, Simons D, Elton L, Haider N, Khan MA, et al. Antimicrobial resistance patterns and risk factors associated with Salmonella spp. isolates from poultry farms in the East Coast of Peninsular Malaysia: a cross-sectional study. Pathogens. 2021;10(9):1160.
Talukder H, Roky SA, Debnath K, Sharma B, Ahmed J, Roy S. Prevalence and antimicrobial resistance profile of Salmonella isolated from human, animal and environment samples in South Asia: a 10-year meta-analysis. J Epidemiol Glob Health. 2023;13(4):637-52.
Nobrega FL, Vlot M, de Jonge PA, Dreesens LL, Beaumont HJE, Lavigne R, et al. Targeting mechanisms of tailed bacteriophages. Nat Rev Microbiol. 2018;16(12):760-73.
Sulakvelidze A, Alavidze Z, Morris JG. Bacteriophage therapy. Antimicrob Agents Chemother. 2001;45(3):649-59.
Batinovic S, Wassef F, Knowler SA, Rice DTF, Stanton CR, Rose J, et al. Bacteriophages in natural and artificial environments. Pathogens. 2019;8(3):100.
Ngu NT, Phuong LNN, Anh LH, Loc HT, Tam NT, Huan PKN, et al. The efficiency of bacteriophages against Salmonella Typhimurium infection in native Noi broilers. Braz J Poult Sci. 2022;24:eRBCA-2022-1794.
Bardina C, Spricigo DA, Cortés P, Llagostera M. Significance of the bacteriophage treatment schedule in reducing Salmonella colonization of poultry. Appl Environ Microbiol. 2012;78(18):6600-7.
Esmael A, Azab E, Gobouri AA, Nasr-Eldin MA, Moustafa MMA, Mohamed SA, et al. Isolation and characterization of two lytic bacteriophages infecting a multi-drug resistant Salmonella Typhimurium and their efficacy to combat salmonellosis in ready-to-use foods. Microorganisms. 2021;9(2):423.
Pinto RM, Soares FA, Reis S, Nunes C, Van Dijck P. Innovative strategies toward the disassembly of the EPS matrix in bacterial biofilms. Front Microbiol. 2020;11:952.
Chegini Z, Khoshbayan A, Taati Moghadam M, Farahani I, Jazireian P, Shariati A. Bacteriophage therapy against Pseudomonas aeruginosa biofilms: a review. Ann Clin Microbiol Antimicrob. 2020;19(1):45.
Maffei E, Woischnig A-K, Burkolter MR, Heyer Y, Humolli D, Thürkauf N, et al. Phage Paride can kill dormant, antibiotic-tolerant cells of Pseudomonas aeruginosa by direct lytic replication. Nat Commun. 2024;15(1):175.
Pelyuntha W, Ngasaman R, Yingkajorn M, Chukiatsiri K, Benjakul S, Vongkamjan K. Isolation and characterization of potential Salmonella phages targeting multidrug-resistant and major serovars of Salmonella derived from broiler production chain in Thailand. Front Microbiol. 2021;12:640993.
Stepanovi? S, Vukovi? D, Hola V, Di Bonaventura G, Djuki? S, Cirkovi? I, et al. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS. 2007;115(8):891-9.
Zar JH. Biostatistical analysis. 5th ed. Upper Saddle River: Prentice Hall; 1999.
Dowah ASA, Clokie MRJ. Review of the nature, diversity and structure of bacteriophage receptor binding proteins that target Gram-positive bacteria. Biophys Rev. 2018;10(2):535-42.
Shin H, Lee J-H, Kim H, Choi Y, Heu S, Ryu S. Receptor diversity and host interaction of bacteriophages infecting Salmonella enterica serovar Typhimurium. PLoS One. 2012;7(8):e43392.
Bertozzi Silva J, Storms Z, Sauvageau D. Host receptors for bacteriophage adsorption. FEMS Microbiol Lett. 2016;363(4):fnw002.
Daubie V, Chalhoub H, Blasdel B, Dahma H, Merabishvili M, Glonti T, et al. Determination of phage susceptibility as a clinical diagnostic tool: a routine perspective. Front Cell Infect Microbiol. 2022;12:974813.
Clavijo V, Morales T, Vives-Flores MJ, Reyes Muñoz A. The gut microbiota of chickens in a commercial farm treated with a Salmonella phage cocktail. Sci Rep. 2022;12(1):991.
Molina F, Menor-Flores M, Fernández L, Vega-Rodríguez MA, García P. Systematic analysis of putative phage-phage interactions on minimum-sized phage cocktails. Sci Rep. 2022;12(1):2458.
Ngiam L, Weynberg KD, Guo J. The presence of plasmids in bacterial hosts alters phage isolation and infectivity. ISME Commun. 2022;2(1):75.
Holtzman T, Globus R, Molshanski-Mor S, Ben-Shem A, Yosef I, Qimron U. A continuous evolution system for contracting the host range of bacteriophage T7. Sci Rep. 2020;10(1):307.
Visnapuu A, Van der Gucht M, Wagemans J, Lavigne R. Deconstructing the phage-bacterial biofilm interaction as a basis to establish new antibiofilm strategies. Viruses. 2022;14(5):1057.
Shree P, Singh CK, Sodhi KK, Surya JN, Singh DK. Biofilms: understanding the structure and contribution towards bacterial resistance in antibiotics. Med Microecol. 2023;16:100084.
Bisht K, Wakeman CA. Discovery and therapeutic targeting of differentiated biofilm subpopulations. Front Microbiol. 2019;10:1908.
Balducci E, Papi F, Capialbi DE, Del Bino L. Polysaccharides' structures and functions in biofilm architecture of antimicrobial-resistant (AMR) pathogens. Int J Mol Sci. 2023;24(4):4030.
Arias CF, Acosta FJ, Bertocchini F, Herrero MA, Fernández-Arias C. The coordination of anti-phage immunity mechanisms in bacterial cells. Nat Commun. 2022;13(1):7412.
Schwartz DA, Lehmkuhl BK, Lennon JT. Phage-encoded sigma factors alter bacterial dormancy. mSphere. 2022;7(4):e00297-22.
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