Assessment of Antioxidant, Antimicrobial and Wound Healing Potential of Golden Chicken Fern (Cibotium barometz (L.) J. Sm.) Methanolic Extracts

Authors

  • Nursyamila Izzatie Rosdin Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • Mohd Ezuan Khayat Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • Suet Lin Chia Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Murni Halim Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • Helmi Wasoh Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • Zulfazli M. Sobri Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

DOI:

https://doi.org/10.54987/jobimb.v12i2.1013

Keywords:

Cibotium barometz, Antioxidant activity, Antimicrobial properties, Wound healing, Bioactive compounds

Abstract

Cibotium barometz (L.) J. Sm., also known as the golden chicken fern, is valued in traditional Chinese medicine, particularly for its rhizome and golden hairs, which are reputed to have medicinal properties. However, there is limited scientific evidence supporting the wound-healing properties of its golden hairs. This study aims to evaluate the antioxidant, antimicrobial, and wound-healing potential of extracts from these hairs. The golden hairs were extracted with 80% methanol to isolate bioactive compounds. Antioxidant activity was measured using the DPPH radical scavenging assay, and total phenolic content (TPC) and total flavonoid content (TFC) were assessed. TFC was found to be higher than TPC, with values of 331 ± 0.011 mg QE/g and 67.36 ± 0.014 mg GAE/g, respectively. The antimicrobial properties of the extract were tested against Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Serratia marcescens using agar well diffusion; no inhibition zones were observed, indicating a lack of antimicrobial activity. Further assays, including cytotoxicity and wound scratch tests on normal human dermal fibroblast (NHDF) cells, revealed that the extract did not promote wound healing and suppressed NHDF cell growth at a concentration of 10 mg/mL. In conclusion, while C. barometz  golden hairs are traditionally valued, this study found limited antioxidant activity and no antimicrobial or wound-healing efficacy in the extract.

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Published

25.12.2024

How to Cite

Rosdin, N. I. ., Khayat, M. E., Chia, S. L., Halim, M., Wasoh, H., & Sobri, Z. M. . (2024). Assessment of Antioxidant, Antimicrobial and Wound Healing Potential of Golden Chicken Fern (Cibotium barometz (L.) J. Sm.) Methanolic Extracts. Journal of Biochemistry, Microbiology and Biotechnology, 12(2), 16–23. https://doi.org/10.54987/jobimb.v12i2.1013

Issue

Vol. 12 No. 2 (2024)

Section

Articles

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Copyright (c) 2024 Nursyamila Izzatie Rosdin, Mohd Ezuan Khayat, Suet Lin Chia, Murni Halim, Helmi Wasoh, Zulfazli M. Sobri

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