Amplification of new isolated luciferase gene from marine Photobacterium strain MIE by using specific PCR

Authors

  • A R Abdul Rachman Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
  • M.I.E. Halmi Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
  • M.Y. Shukor Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia

DOI:

https://doi.org/10.54987/jemat.v2i1.92

Keywords:

Photobacterium strain MIE, luciferase gene, polymerase chain reaction, PCR primer

Abstract

The aim of this study is to isolate a new luciferase gene from Photobacterium strain MIE by using specific polymerase chain reaction (PCR) and to identify whether this new Photobacterium sp. same strain as P.kishitanii or P.phosphoreum. The Photobacterium strain MIE was successfully isolated, characterized and identified in 2012. After analyzing by using blastn of 16S rRNA, this strain was closely related with several Photobacterium kishitanii and Photobacterium phosphoreum strain. Primers were designed based on the nucleotide multiple sequence alignment of luciferase gene from several Photobacterium from both species deposited in Genebank. Luciferase genes from P.kishitanii and P.phosphoreum have the expected size of ~1.8 kbp and ~2.1 kbp, respectively. Result shown that, both size were amplified by using specific primers

Author Biography

A R Abdul Rachman, Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia

Department of Biochemistry

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Published

29.07.2014

How to Cite

Abdul Rachman, A. R., Halmi, M., & Shukor, M. (2014). Amplification of new isolated luciferase gene from marine Photobacterium strain MIE by using specific PCR. Journal of Environmental Microbiology and Toxicology, 2(1), 35–37. https://doi.org/10.54987/jemat.v2i1.92

Issue

Vol. 2 No. 1 (2014)

Section

Articles

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