The evaluation of Periophtalmodon schlosseri as a source of acetylcholinesterase for the detection of insecticides

Authors

  • M.K. Sabullah Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • S.A. Ahmad Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • M.Y. Shukor Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • M.A. Syed Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • N.A. Shamaan Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, 55100 USIM, Kuala Lumpur, Malaysia.

DOI:

https://doi.org/10.54987/bessm.v1i1.994

Keywords:

Periophtalmodon Schlosseri, Acetylcholinesterase, Carbamate, Organophosphate

Abstract

The aim of this study is to investigate the ability of acetylcholinesterase from mudskipper, Periophtalmodon schlosseri, as an assay to detect various pesticides, especially carbamate and organophosphate, and selected metal ions. The assay is sensitive to several carbamates with IC50of 0.0993, 0.0945, 0.0532 and 0.0488 mg/L for carbaryl, methomyl, propoxur and bendiocarb, respectively. Carbofuran highly inhibited the activity (0.0313) with the limit of detection (LOD) of 0.0176 and the limit of quantitation (LOQ) of 0.0526. The organophosphate group is less inhibiting compared to carbamate group.

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Published

2013-12-31

How to Cite

Sabullah, M., Ahmad, S., Shukor, M., Syed, M., & Shamaan, N. (2013). The evaluation of Periophtalmodon schlosseri as a source of acetylcholinesterase for the detection of insecticides. Bulletin of Environmental Science and Sustainable Management (e-ISSN 2716-5353), 1(1), 20–24. https://doi.org/10.54987/bessm.v1i1.994

Issue

Vol. 1 No. 1 (2013)

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