Entrapment of Mo-Reducing Bacterium Increase Its Resistance towards Heavy Metals

Authors

  • M.I.E. Halmi Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • S.A. Ahmad Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • M.T. Yusor Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • M.Y. Shukor Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • M.A. Syed Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.

DOI:

https://doi.org/10.54987/bessm.v1i1.992

Keywords:

Molybdenum, pollution, Dialysis, Tubing

Abstract

Molybdenum is highly toxic to ruminant at several ppm. Molybdenum pollution in soil and mine tailings could reach 20,000 ppm in the most contaminated region. Molybdenum is mined as a byproduct of copper in malaysia and occasional elevated levels of these heavy metals have been reported. Bioremediation of molybdenum in the presence of the toxic copper is a challenge. In this work a novel method of molybdenum removal from aqueous solution using the dialysis tubing method coupled with molybdenum-reducing activity of serratia sp. Strain dry5 is demonstrated. The enzymatic reduction of molybdenum is molybdenum blue, a colloid that does not pass through dialysis tubing. The calculated maximal rate of molybdenum blue production (vmobluemax) was 0.264±0.034 mmole/mo-blue/hr and the concentration of molybdate resulting in the half- maximal rate of reduction (kmo) was 21.78±3.89 mM molybdate indicating an efficient system with high tolerance towards molybdenum. Heavy metals exhibited significantly higher inhibition towards free cells compared to dialysis tubing entrapped cells. Hence the immobilization of mo- reducing cells by entrapment could be a viable bioremediation tool in aquatic bodies or effluent co-contaminated by other heavy metals.

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Published

2013-12-31

How to Cite

Halmi, M., Ahmad, S., Yusor, M., Shukor, M., & Syed, M. (2013). Entrapment of Mo-Reducing Bacterium Increase Its Resistance towards Heavy Metals. Bulletin of Environmental Science and Sustainable Management (e-ISSN 2716-5353), 1(1), 11–13. https://doi.org/10.54987/bessm.v1i1.992

Issue

Vol. 1 No. 1 (2013)

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