An inhibitive assay for insecticides using the acetylcholinesterase from Osteochillus hasselti

Authors

  • M.K. Sabullah School of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia.
  • S.A. Ahmad Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • I. Ishak Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • M.R. Sulaiman School of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia.
  • M.Y. Shukor Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • M.A. Syed Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • N.A. Shamaan Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, 55100 USIM, Kuala Lumpur, Malaysia.

DOI:

https://doi.org/10.54987/bessm.v1i1.990

Keywords:

Osteochillus hasselti, Acetylcholinesteras, Carbamates, Organophosphates

Abstract

The aim of this study is to investigate the ability of acetylcholinesterase from fresh water carp, Osteochillus hasseltias an assay to detect insecticides.The IC50 values for the carbamates carbofuran, carbaryl, methomyl, bendiocarb were 0.0550, 0.0497, 0.0845, 0.0470µg/l, respectively, and the IC50 values for the oxonated organophosphates parathion, malathion, diazinon and chlorpyrifos were 0.0660, 0.0681, 0.0991 and 0.0632µg/l, respectively. The carbamates carbaryl, and the oxonated organophosphate diazinon showed lower IC50s in O. hasselti compared to E. electricus while parathion and diazinon showed similar sensitivity to E. electricus. Carbofuran, methomyl, bendiocarb and malathion exhibited lower IC50 confidence interval in E. electricus than in O. hasselti. This suggests that in overall, AChE from Osteochillus hasseltiis a suitable source of enzyme for the detection of insecticides.

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Published

2013-12-31

How to Cite

Sabullah, M., Ahmad, S., Ishak, I., Sulaiman, M., Shukor, M., Syed, M., & Shamaan, N. (2013). An inhibitive assay for insecticides using the acetylcholinesterase from Osteochillus hasselti. Bulletin of Environmental Science and Sustainable Management (e-ISSN 2716-5353), 1(1), 1–4. https://doi.org/10.54987/bessm.v1i1.990

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Vol. 1 No. 1 (2013)

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