Characterization of a Metanil Yellow-decolorizing Pseudomonas strain Isolated from the Juru Industrial Park
DOI:
https://doi.org/10.54987/bessm.v7i2.917Keywords:
Metanil Yellow-decolorizing, Pseudomonas strain, Juru Industrial Park, Characterization, BioremediationAbstract
This study examines how the initial concentration of dye, temperature, pH, and NaCl content affect the ability of a bacterial strain, specifically identified as Pseudomonas sp. strain UPM291, to remove color from Metanil Yellow. The decolorization percentage exhibited a distinct trend throughout a range of dye concentrations (0-700 mg/L), with the greatest efficacy (90-100%) recorded at values below 200 mg/L. The efficiency declined at higher concentrations, reaching approximately 20% at a dosage of 700 mg/L. Temperature investigations unveiled a symmetrical curve resembling a bell shape, indicating a range of temperatures that is most favorable for the process of decolorization. The maximum efficiency, approaching 100%, was found at a temperature of 35°C. However, the efficiency decreased considerably as the temperature above 35°C, reaching approximately 20% at 50°C. The impact of pH on decolorization exhibited comparable patterns, with optimal efficacy observed at pH 6.5 and diminished efficacy at both more acidic and more alkaline settings. The decolorization efficiency reached its peak (90-100%) at a pH of 6.5 and decreased to approximately 60% at a pH of 8.0. The influence of the NaCl content on decolorization was shown to follow a certain pattern, with the most effective decolorization (90-100%) occurring at NaCl concentrations of up to 10 g/L. The efficiency declined as the NaCl concentrations increased, reaching around 20% at 30 g/L. The data indicate that the bacterial strain demonstrates the highest effectiveness in removing color within particular ranges of dye concentration, temperature, pH, and NaCl content. There is a noticeable decline in efficiency when the parameters go outside of these optimal ranges. Gaining a comprehensive understanding of these characteristics can assist in optimizing the conditions necessary for the efficient bioremediation of Metanil Yellow utilizing specific bacterial strains.
References
Abd Shukor MS, Aftab K, Norazlina M, Effendi Halmi M, Sheikh A, Shukor M. Isolation of a Novel Molybdenum-reducing and Azo Dye Decolorizing Enterobacter sp. Strain Aft-3 from Pakistan. Chiang Mai Univ J Nat Sci. 2016 Jan 1;15:95-114.
Dan-Iya BI, Basirun AA, Shukor MY. Kinetic Analysis of the Adsorption of Ethyl Violet onto Graphene Oxide Sheets Integrated with Gold Nanoparticles. Bioremediation Sci Technol Res. 2021 Dec 31;9(2):35-9.
Manogaran M, Yasid NA, Othman AR, Gunasekaran B, Halmi MIE, Shukor MYA. Biodecolourisation of Reactive Red 120 as a Sole Carbon Source by a Bacterial Consortium-Toxicity Assessment and Statistical Optimisation. Int J Environ Res Public Health. 2021 Jan;18(5):2424.
Eskandari F, Shahnavaz B, Mashreghi M. Optimization of complete RB-5 azo dye decolorization using novel cold-adapted and mesophilic bacterial consortia. J Environ Manage. 2019;241:91-8.
Farag S, Bekhit F, Attia AM. Bacterial isolation, optimization and immobilization for decolorization and degradation of the azo dye (basic red 46). Res J Pharm Biol Chem Sci. 2016;7(4):1323-35.
Guo G, Li X, Tian F, Liu T, Yang F, Ding K, et al. Azo dye decolorization by a halotolerant consortium under microaerophilic conditions. Chemosphere [Internet]. 2020;244. Available from: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85076057919&doi=10.1016%2fj.chemosphere.2019.125510&partnerID=40&md5=03084a46ed5d7d42bc674520dbd5f922
Kamble KD, More MA. Bacterial decolorization of acid yellow dye obtained from textile industry effluents. Int J Pharma Bio Sci. 2013;4(4):B763-9.
Malik NH, Gough HL. Textile Dye Wastewater Biotreatment-A Project Empowering Female Leadership through International Exchange. In 2018. p. 238-49. Available from: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048866967&doi=10.1061%2f9780784481394.023&partnerID=40&md5=a2205f366cb6b5265d9ed6d9379003e4
Raghavendra KR, Ajay Kumar K. Synthesis of some novel azo dyes and their dyeing, redox and antifungal properties. Int J ChemTech Res. 2013;5(4):1756-60.
Palanivelan R, Rajakumar S, Ayyasamy PM. Effect of various carbon and nitrogen sources on decolorization of textile dye remazol golden yellow using bacterial species. J Environ Biol. 2014;35(5):781-7.
Senthilvelan T, Kanagaraj J, Panda RC. Enzyme-Mediated Bacterial Biodegradation of an Azo Dye (C.I. Acid Blue 113): Reuse of Treated Dye Wastewater in Post-Tanning Operations. Appl Biochem Biotechnol. 2014;174(6):2131-52.
Deka A, Chougule BS, Parveen A, Lahan JP, Barooah M, Boro RC. Natural pigment betacyanin as tracking dye for gel electrophoresis. Indian J Nat Prod Resour. 2015;6(1):23-6.
Zhang X, Jing J, Zhang L, Song Z, Zhou H, Wu M, et al. Biodegradation characteristics and genomic functional analysis of indole-degrading bacterial strain Acinetobacter sp. JW. J Chem Technol Biotechnol. 2019;94(4):1114-22.
Xu B, Sun QJ, Lan JCW, Chen WM, Hsueh CC, Chen BY. Exploring the glyphosate-degrading characteristics of a newly isolated, highly adapted indigenous bacterial strain, Providencia rettgeri GDB 1. J Biosci Bioeng. 2019;128(1):80-7.
Win YY, Singh M, Sadiq MB, Anal AK. Isolation and identification of caffeine-degrading bacteria from coffee plantation area. Food Biotechnol. 2019;33(2):109-24.
Sandhyarani R, Mishra S. Isolation and characterization of phenol degrading organism, optimization using doehlert design. Desalination Water Treat. 2019;148:351-62.
Chaturvedi V, Kumar A. Isolation of sodium dodecyl sulfate degrading strains from a detergent polluted pond situated in Varanasi city, India. J Cell Mol Biol. 2010;8(2):103-11.
Chaturvedi V, Kumar A. Diversity of culturable sodium dodecyl sulfate (SDS) degrading bacteria isolated from detergent contaminated ponds situated in Varanasi city, India. Int Biodeterior Biodegrad. 2011;65(7):961-71.
Lee M, Woo SG, Ten LN. Characterization of novel diesel-degrading strains Acinetobacter haemolyticus MJ01 and Acinetobacter johnsonii MJ4 isolated from oil-contaminated soil. World J Microbiol Biotechnol. 2012;28(5):2057-67.
Kafilzadeh F, Mokhtari S. Isolation and identification of phenol degrading bacteria from mangrove sediments in the persian gulf (asaluyeh) and their growth kinetics assay. Biomed Pharmacol J. 2013;6(2):189-96.
Kumari S, Chetty D, Ramdhani N, Bux F. Phenol degrading ability of Rhodococcus pyrinidivorans and Pseudomonas aeruginosa isolated from activated sludge plants in South Africa. J Environ Sci Health - Part ToxicHazardous Subst Environ Eng. 2013;48(8):947-53.
Motiwalla MJ a, Punyarthi PP b, Mehta MK b, D'Souza JS a, Kelkar-Mane V b. Studies on degradation efficiency of polycaprolactone by a naturally-occurring bacterium. J Environ Biol. 2013;34(1):43-9.
Shahbazi R, Kasra-Kermanshahi R, Gharavi S, Moosavi- Nejad Z, Borzooee F. Screening of SDS-degrading bacteria from car wash wastewater and study of the alkylsulfatase enzyme activity. Iran J Microbiol. 2013;5(2):153-8.
Charoenpanich J, Tani A. Proteome analysis of acrylamide-induced proteins in a novel acrylamide-degrader Enterobacter aerogenes by 2D electrophoresis and MALDI-TOF-MS. Chiang Mai Univ J Nat Sci. 2014;13(1):11-22.
Sarkar R, Ghosh AR. Metanil Yellow, a food additive induces the responses at cellular and sub-cellular organisations of stomach, intestine, liver, and kidney of heteropneustes fossilis (bloch). EM Int. 2010 Jan 1;29:453-60.
Sarkar R, Ghosh A, Bengal W. Metanil Yellow - an azo dye induced histopathological and ultrastructural changes in albino rat (Rattus norvegicus). The Bioscan. 2012;7(3):427-32.
Nath PP, Sarkar K, Mondal M, Paul G. Metanil Yellow impairs the estrous cycle physiology and ovarian folliculogenesis in female rats. Environ Toxicol. 2016 Dec;31(12):2057-67.
Dome R, Hazra S, Ghosh D, Ghosh S. Beneficial Effects of Ethanolic Leaf Extract of Coriandrum Sativum on Metanil Yellow induced alteration in activity of Catalase and Level of Lipid Peroxidation in Hercine Cardiac tissue In Vitro. Int J Pharm Pharm Sci. 2017 May 1;9.
Nagaraja TN, Desiraju T. Effects of chronic consumption of Metanil Yellow by developing and adult rats on brain regional levels of noradrenaline, dopamine and serotonin, on acetylcholine esterase activity and on operant conditioning. Food Chem Toxicol Int J Publ Br Ind Biol Res Assoc. 1993 Jan;31(1):41-4.
Ramchandani S, Das M, Joshi A, Khanna SK. Effect of oral and parenteral administration of Metanil Yellow on some hepatic and intestinal biochemical parameters. J Appl Toxicol. 1997;17(1):85-91.
Hazra S, Dome R, Ghosh S, Ghosh D. Protective effect of methanolic leaves extract of Coriandrum sativum against Metanil Yellow induced lipid peroxidation in goat liver: an in vitro study. 2016 Sep 12;3:34-41.
Jain K, Shah V, Chapla D, Madamwar D. Decolorization and degradation of azo dye - Reactive Violet 5R by an acclimatized indigenous bacterial mixed cultures-SB4 isolated from anthropogenic dye contaminated soil. J Hazard Mater. 2012;213-214:378-86.
Ng IS, Chen T, Lin R, Zhang X, Ni C, Sun D. Decolorization of textile azo dye and Congo red by an isolated strain of the dissimilatory manganese-reducing bacterium Shewanella xiamenensis BC01. Appl Microbiol Biotechnol. 2014;98(5):2297-308.
Guo G, Hao J, Tian F, Liu C, Ding K, Zhang C, et al. Decolorization of Metanil Yellow G by a halophilic alkalithermophilic bacterial consortium. Bioresour Technol. 2020 Nov;316:123923.
Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergeys Man Determinative Bacteriol. 1994;
Costin S, Ionut S. ABIS online - bacterial identification software, http://www.tgw1916.net/bacteria_logare.html, database version: Bacillus 022012-2.10, accessed on Mar 2015. 2015.
Shukor MS, Shukor MY. A microplate format for characterizing the growth of molybdenum-reducing bacteria. J Environ Microbiol Toxicol. 2014;2(2):42-4.
Solís M, Solís A, Pérez HI, Manjarrez N, Flores M. Microbial decolouration of azo dyes: A review. Process Biochem. 2012;47(12):1723-48.
Anjaneya O, Souche SY, Santoshkumar M, Karegoudar TB. Decolorization of sulfonated azo dye Metanil Yellow by newly isolated bacterial strains: Bacillus sp. strain AK1 and Lysinibacillus sp. strain AK2. J Hazard Mater. 2011 Jun 15;190(1):351-8.
Chang JS, Chou C, Lin YC, Lin PJ, Ho JY, Hu TL. Kinetic characteristics of bacterial azo-dye decolorization by Pseudomonas luteola. Water Res. 2001;35(12):2841-50.
Chen BY, Chen SY, Lin MY, Chang JS. Exploring bioaugmentation strategies for azo-dye decolorization using a mixed consortium of Pseudomonas luteola and Escherichia coli. Process Biochem. 2006;41(7):1574-81.
Chen JP, Lin YS. Decolorization of azo dye by immobilized Pseudomonas luteola entrapped in alginate-silicate sol-gel beads. Process Biochem. 2007;42(6):934-42.
Hsueh CC, Chen BY. Comparative study on reaction selectivity of azo dye decolorization by Pseudomonas luteola. J Hazard Mater. 2007;141(3):842-9.
Joe J, Kothari RK, Raval CM, Kothari CR. Decolorization of textile dye Remazol Black B by Pseudomonas aeruginosa CR-25 isolated from the common effluent treatment plant. J Bioremediation Biodegrad. 2011;02(02):118.
Kumar Garg S, Tripathi M, Singh SK, Tiwari JK. Biodecolorization of textile dye effluent by Pseudomonas putida SKG-1 (MTCC 10510) under the conditions optimized for monoazo dye orange II color removal in simulated minimal salt medium. Int Biodeterior Biodegrad. 2012;74:24-35.
Zablocka-Godlewska E, Przystas W, Grabinska-Sota E. Decolourization of diazo evans blue by two strains of Pseudomonas fluorescens isolated from different wastewater treatment plants. Water Air Soil Pollut. 2012;223(8):5259-66.
Hafshejani MK, Ogugbue CJ, Morad N. Sequential microaerophilic-oxic phase mineralization of Azo dyes by a monoculture of Pseudomonas aeruginosa strain AWF isolated from textile wastewater. Water Air Soil Pollut. 2013;224(9).
Hussain S, Maqbool Z, Ali S, Yasmeen T, Imran M, Mahmood F, et al. Biodecolorization of reactive black-5 by a metal and salt tolerant bacterial strain Pseudomonas sp. RA20 isolated from Paharang drain effluents in Pakistan. Ecotoxicol Environ Saf. 2013;98:331-8.
Jadhav SB, Surwase SN, Phugare SS, Jadhav JP. Response surface methodology mediated optimization of Remazol Orange decolorization in plain distilled water by Pseudomonas aeruginosa BCH. Int J Environ Sci Technol. 2013;10(1):181-90.
Rusnam, Gusmanizar N. Isolation and Characterization of a Molybdenum-reducing and the Congo Red Dye-decolorizing Pseudomonas putida strain Neni-3 in soils from West Sumatera, Indonesia. J Biochem Microbiol Biotechnol. 2022 Jul 31;10(1):17-24.
Mansur R, Gusmanizar N, Roslan MAH, Ahmad SA, Shukor MY. Isolation and characterisation of a molybdenum-reducing and Metanil Yellow dye-decolourising Bacillus sp. strain Neni-10 in soils from West Sumatera, Indonesia. Trop Life Sci Res. 2017 Jan;28(1):69-90.
Lal N, Chand S. Decolorization of Sulphonated Azodye Metanil Yellow by Newly Isolated Bacterial Strain: Bacillus -3330. J Environ Sci Eng. 2014 Jul;56(3):341-6.
Muliadi FNA, Halmi MIE, Wahid SBA, Gani SSA, Zaidan UH, Mahmud K, et al. Biostimulation of Microbial Communities from Malaysian Agricultural Soil for Detoxification of Metanil Yellow Dye; a Response Surface Methodological Approach. Sustainability. 2021 Jan;13(1):138.
El Ahwany AMD. Decolorization of Fast red by metabolizing cells of Oenococcus oeni ML34. World J Microbiol Biotechnol. 2008;24(8):1521-7.
Vijaykumar MH, Vaishampayan PA, Shouche YS, Karegoudar TB. Decolourization of naphthalene-containing sulfonated azo dyes by Kerstersia sp. strain VKY1. Enzyme Microb Technol. 2007;40(2):204-11.
Jadhav SU, Kalme SD, Govindwar SP. Biodegradation of Methyl red by Galactomyces geotrichum MTCC 1360. Int Biodeterior Biodegrad. 2008 Sep 1;62(2):135-42.
Ramlan MAM, Azizan NA, Han BH, Kim LC, Mohamad SE, Ibrahim Z. Decolourisation of reactive black 5 by azoreductase produced by BreviBacillus panacihumi ZBI. J Teknol Sci Eng. 2012;59(SUPPL.1):11-6.
Ogugbue CJ, Sawidis T, Oranusi NA. Bioremoval of chemically different synthetic dyes by Aeromonas hydrophila in simulated wastewater containing dyeing auxiliaries. Ann Microbiol. 2012;62(3):1141-53.
Wong PK, Yuen PY. Decolorization and biodegradation of methyl red by Klebsiella pneumoniae RS-13. Water Res. 1996;30(7):1736-44.
Song XY, Liu FJ, Zhou HB, Yang HL. Biodegradation of Acid Scarlet 3R by a New Salt-tolerant Strain Alcaligenes faecalis LJ-3: Character, Enzyme and Kinetics Analysis. Chem Biochem Eng Q. 2018 Oct 13;32(3):371-81.
Gao F, Ding H, Feng Z, Liu D, Zhao Y. Functional display of triphenylmethane reductase for dye removal on the surface of Escherichia coli using N-terminal domain of ice nucleation protein. Bioresour Technol. 2014;169:181-7.
Pathak H, Madamwar D. Biosynthesis of indigo dye by newly isolated naphthalene-degrading strain pseudomonas sp. HOB1 and its application in dyeing cotton fabric. Appl Biochem Biotechnol. 2010;160(6):1616-26.
Chen G, An X, Feng L, Xia X, Zhang Q. Genome and transcriptome analysis of a newly isolated azo dye degrading thermophilic strain AnoxyBacillus sp. Ecotoxicol Environ Saf. 2020 Oct 15;203:111047.
Guo G, Li X, Tian F, Liu T, Yang F, Ding K, et al. Azo dye decolorization by a halotolerant consortium under microaerophilic conditions. Chemosphere. 2020 Apr;244:125510.
Beydilli MI, Pavlostathis SG. Biodecolorization of the Azo Dye Reactive Red 2 by a Halotolerant Enrichment Culture. Water Environ Res. 2007;79(12):2446-56.
Asad S, Amoozegar MA, Pourbabaee AA, Sarbolouki MN, Dastgheib SMM. Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria. Bioresour Technol. 2007;98(11):2082-8.
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