Response Surface Method for the Optimization of Glyphosate Degrada-tion by Bacillus sp. Isolated from Soils Near Lake Maninjau
DOI:
https://doi.org/10.54987/bessm.v7i2.916Keywords:
Glyphosate-degrading bacterium, Bacillus sp., Bioremediation, RSM, Box-BehnkenAbstract
The study utilized a Box-Behnken experimental design to assess the impact of three parameters on the percentage of glyphosate biodegradation by a bacterium. The variables examined were incuba-tion period (measured in days), glyphosate concentration (measured in grams per liter), and pH, each assessed at three different levels. The quadratic model, which has squared terms, interaction products, linear terms, and an intercept, most accurately characterizes the connection between the variables and the response. The findings demonstrated that glyphosate concentration exerted the most pronounced impact on glyphosate degradation, with pH ranking second, as supported by substantial F-values and low p-values. The incubation period had no discernible effect. The ANOVA analysis validated the dependability of the model, as evidenced by an R² value of 0.9602 and an adjusted R² value of 0.9091. These values indicate that the model accounts for 90% of the variability observed in the response data. The contour and response surface plots demon-strated substantial interactions among the variables, specifically between pH and glyphosate con-centration and between the incubation period and glyphosate concentration. The model's anticipat-ed optimal conditions were experimentally tested, demonstrating no significant deviation from the projected values. The predicted maximum biodegradation of 90.097% closely matched the exper-imentally observed value of 92.505% (p>0.05). The predicted combination to give the desired maximum response was at pH 6.81, glyphosate concentration of 0.692 g/L and an incubation period of 3.092 days. On the other hand, the predicted combination to give the desired maximum response is based on the requirement for the conditions where biodegradation is at the highest possible glyphosate concentration of 0.844 g/L and an incubation period of 3.112 days. A higher response of about 5.779% degradation was achieved through RSM. This study showcases the efficacy of the Box-Behnken design in optimizing biodegradation processes, offering a solid sta-tistical basis for further investigations on glyphosate degradation.
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