Isolation and Characterization of a Diesel-degrading Bacterium From Waters Near the Langkawi UNESCO Kilim Karst Geoforest Park
DOI:
https://doi.org/10.54987/bessm.v7i2.915Keywords:
Isolation, Characterization, Bioremediation, Diesel-degrading bacterium, Bacillus sp.Abstract
This study explored the biodegradation potential of bacterial isolates from various locations in Malaysia, focusing on their ability to utilize diesel as a carbon source. Among the ten isolates tested, isolate 4, identified as Pseudomonas sp. strain UPM-Langkawi 3 based on morphological, cultural, and biochemical properties, exhibited significant growth on 2.5% diesel, indicating rapid activation of diesel assimilation mechanisms. Using the ABIS online software analysis, the strain was provisionally identified as Pseudomonas sp. UPM-Langkawi 3. Experimental results demonstrated that the optimal conditions for the growth of strain UPM-Langkawi 3 were between 6 and 8 % (v/v) diesel concentration, a temperature of between 28 and 35 °C, and a pH range of between 7.5 to 8.5. The strain showed inhibited growth at higher diesel concentrations and temperatures above 40 °C, as well as at a lower pH of 5.7. Among several inorganic nitrogen sources tested, 0.9% w/v ammonium sulphate was found to be the most effective, corroborating previous research. The study underscores the efficacy of Pseudomonas sp. strain UPM-Langkawi 3 in degrading diesel, suggesting its potential use in bioremediation of diesel-contaminated environments. Further research should employ molecular identification techniques, such as 16srRNA gene sequencing, to confirm the species identity and optimize biodegradation conditions. This work establishes a foundation for utilizing indigenous bacterial strains in environmental management strategies, particularly for areas impacted by hydrocarbon pollutants.
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