Characterization of a Glyphosate-degrading Bacterium from a Paddy Field in Kepala Batas, Penang
DOI:
https://doi.org/10.54987/bessm.v7i1.901Keywords:
Bioremediation, Glyphosate, Pollution, Pseudomonas sp., Heavy metalAbstract
Bioremediation of contaminants, including glyphosate, a herbicide, is an economically viable and environmentally friendly technique. Glyphosate is the most utilized herbicides for weed management. Pollution from glyphosate is dangerous to wildlife and their habitats. Soil Pseudomonas sp. strain UMP-KB2 obtained from a paddy field was used as the only source of carbon and described for its capacity to degrade glyphosate. The growth of these bacteria was measured spectrophotometrically as A600 nm in response to changes in incubation time, g inoculum size, glyphosate concentration (carbon source), temperature and pH. The bacterium degrades glyphosate optimally at pH 7.0, glyphosate concentration of 0.5 g/L, temperatures of between 30 and 35 ºC, and inoculum size 1% (v/v). Growth at 0.5 g/L glyphosate shows a two-day lag period and nearly 90% degradation after six days of incubation. The isolation of a glyphosate-degrading bacterium that utilizes glyphosate as a carbon source will be very useful in mineralizing glyphosate in contaminated agriculture soil.
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