Modelling the Inhibitory Effect of Mercury on the Growth Rate of a Bacterium on Acrylamide
DOI:
https://doi.org/10.54987/bessm.v7i1.899Keywords:
Mercury, Acrylamide-degrading bacterium, Pseudomonas, Inhibition kinetics, Mathematical modelsAbstract
Mercury was a potent inhibitor of the acrylamide-degrading Pseudomonas sp. strain DrY Kertih bacterium. The bacterium is capable of growing on acrylamide as a nitrogen source. Growth shows a sigmoidal pattern with lag times of 7–10 hours when exposed to increasing amounts of mercury. Bacterial growth was significantly slowed down while the lag period was increased as the concentration of mercury was increased to 0.1 mg/L, and eventually, growth stopped altogether. Growth rates at various mercury concentrations were calculated using a modified Gompertz model to obtain the maximum specific growth rate parameter. The modified Han-Levenspiel, modified Andrews, Shukor, Kai, Liu, Wang and Amor models were applied to the growth rates. The Amor model was unable to fit the curve. The Wang model performed the best statistically, with the lowest RMSE and AICc, the highest adjusted correlation coefficient (adR2), and AF and BF values closest to unity. The parameters obtained were Kc, max and m which represent critical heavy metal ion concentration (g/l), and maximum growth rate (g/l h) and empirical constant values were KC, max and m which represent inhibition constant (mg/L), maximum growth rate (per h) and empirical constant values were 0.054, 0.431 and 4.295, respectively. The results obtained in this study are useful when remediation works on polluted sites containing acrylamide co-contaminated with metal ions especially mercury.
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