Characterization of carbazole degrading marine bacterium strain OC6S isolated from seawater of Kobe, Japan, a probable novel species and genus from‘Alphaproteobacteria’class

Authors

  • Azham . Zulkharnain Dept. of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
  • Hiroyuki Fuse College of Graduate School of Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minuma-ku, 337-8570 Saitama, Japan.
  • Rintaro Maeda College of Graduate School of Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minuma-ku, 337-8570 Saitama, Japan.
  • Shintaro Oba College of Graduate School of Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minuma-ku, 337-8570 Saitama, Japan.
  • Toshio Omori College of Graduate School of Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minuma-ku, 337-8570 Saitama, Japan.

DOI:

https://doi.org/10.54987/bessm.v2i1.114

Abstract

The carbazole-degrading marine bacterium OC6ST was isolated from seawater collected off the coast of Kobe, Japan. Phylogenetic analysis of 16S rRNA revealed that this strain is distinct from other known orders of class ‘Alphaproteobacteria’. The closest related bacterium was Kordiimonas gwangyangensis GW14-5T, although 16S rRNA similarity was only 90.1%. Strain OC6ST was Gram-negative, motile, rod-shaped, catalase-positive, oxidase-positive, nitrate-reducing, and mesophilic. Respiratory quinone was Q-10 for both aerobic and anaerobic growth, and the DNA G+C content was 58.3 mol%. Optimal cell growth was observed at 16-37 °C, pH 5.5-10, and at NaCl concentrations up to 5%. The dominant fatty acids were C18 : 1 ω7c (41.54%), C14 : 0 2-OH (14.21%), and C17 : 1 ω6c (10.10%). The results suggest that this marine bacterium may be representing a novel genus and species within ‘Alphaproteobacteria’.

Published

2014-07-30

How to Cite

Zulkharnain, A. ., Fuse, H., Maeda, R., Oba, S., & Omori, T. (2014). Characterization of carbazole degrading marine bacterium strain OC6S isolated from seawater of Kobe, Japan, a probable novel species and genus from‘Alphaproteobacteria’class. Bulletin of Environmental Science and Sustainable Management (e-ISSN 2716-5353), 2(1), 12–16. https://doi.org/10.54987/bessm.v2i1.114

Issue

Vol. 2 No. 1 (2014)

Section

Articles

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