Performance of β-Glucosidase produced by Ganoderma lucidum using Waste Substrate as Carbon Source

Authors

  • Nur Haziqah Aniyah Salihan Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Arbakariya Ariff Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Mohd Rafein Zakaria Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Suraini Abd-Aziz Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Md Noor bd Wahab Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Helmi Wasoh Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

DOI:

https://doi.org/10.54987/jobimb.v1i1.380

Keywords:

Ganoderma lucidum, Beta-glucosidase, waste, compost, activity

Abstract

Oil palm empty fruit bunch (OPEFB) was used for the production of β-glucosidase by Ganoderma lucidum in submerged fermentation. The activity of β-glucosidase had shown an improvement by going from pH 4 to 6 and declined towards alkaline pH. The highest activity (47.75 U/mL) was obtained at pH 6 with 2.0 mm size of substrate’s particle. The smaller the particles size, the higher surface area of substrate which provides more space for hyphae to colonize. Optimum substrate concentration was obtained at 6.0 g/L (43.99 U/mL). The highest β-glucosidase activity occurred at 27 ºC (53.37 U/mL). The nutrient content in composted OPEFB provides a relevant reason that should be considered in the performance of high activity β-glucosidase enzyme. Therefore, the results of this study can be used to produce high activity β-glucosidase using cheap and economical substrate (compost OPEFB).

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Published

31.12.2013

How to Cite

Salihan, N. H. A., Ariff, A., Zakaria, M. R., Abd-Aziz, S., bd Wahab, M. N., & Wasoh, H. (2013). Performance of β-Glucosidase produced by Ganoderma lucidum using Waste Substrate as Carbon Source. Journal of Biochemistry, Microbiology and Biotechnology, 1(1), 17–24. https://doi.org/10.54987/jobimb.v1i1.380

Issue

Vol. 1 No. 1 (2013)

Section

Articles

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