Comparison of staining methods for two dimensional electrophoresis gel resolved with <i>Puntius javanicus</i> liver proteome

Authors

  • Khalizan Sabullah Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia
  • S.A. Ahmad Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
  • Fisal Ahmad Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia
  • Siti Aimi Farah Zainal Abidin Halal Product Research Institute, Putra Infopo1t, UPM 43400 Serdang, Selangor
  • Azlan Jualang Gansau Faculty of Science andNatural Resources, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia.
  • Mohd Rosni Sulaiman Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia

DOI:

https://doi.org/10.54987/jobimb.v2i1.127

Keywords:

P. javanicus, Proteomic, CBB R -250, CBB G-250, Silver nitrate

Abstract

The aim of this study was to compare the various staining methods based on coomassie briliant blue and silver nitrate stain for the two-dimensional gel electrophoresis resolved with Puntius javanicus liver proteome. The staining methods were selected base on the previous report about their compatibility with the mass spectrometry analysis. Silver staining method is known as the most sensitive method to visualize the maximum number of protein spots resolved in 2D gel but it is less sensitive(incompatible) toward mass spectrometry detection. Results of this study showed that a modified staining method using colloidal coomassie blue G-250 (CCB) is roughly similarly sensitive but lower protein spot detected compared with silver staining (SS) as indicated at the number of 303±26 and 693±14 of protein resolved in both types of stained gels. The conventional methods of staining using coomassie brilliant blue G-250 and R-250 only detected less number of protein spots(128±17and 78±11, respectively) compared to modified CCB staining method. As the coomassie brilliant blue stain is known to be a very sensitive for mass spectrometry detection,  the modified method of CCB was selected for further study on Puntius javanicus liver proteome.

Downloads

Published

31.07.2014

How to Cite

Sabullah, K., Ahmad, S., Ahmad, F., Abidin, S. A. F. Z., Gansau, A. J., & Sulaiman, M. R. (2014). Comparison of staining methods for two dimensional electrophoresis gel resolved with <i>Puntius javanicus</i> liver proteome. Journal of Biochemistry, Microbiology and Biotechnology, 2(1), 27–31. https://doi.org/10.54987/jobimb.v2i1.127

Issue

Vol. 2 No. 1 (2014)

Section

Articles

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).