A confidence interval comparison chart to assess the sensitivity of acetylcholinesterase from Hemibagrus nemurus as a source of enzyme for insecticides detection

Authors

  • Nurul Aida Muhamad Asri Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia.
  • Ghousia Begum Toxicology Unit, Biology Division, Indian Institute of Chemical Technology, Tarnaka Hyderabad, 500 007 A.P; India.
  • Siti Aqlima Ahmad Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia.
  • Nur Adeela Yasid Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia.
  • Mohd Yunus Shukor Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia.

DOI:

https://doi.org/10.54987/jemat.v5i1.416

Keywords:

Acetylcholinesterase, Hemibagrus nemurus, Shukor’s chart, insecticides, inhibitive assay

Abstract

In this work, the potential of acetylcholinesterase (AChE) from Hemibagrus nemurus (Baung) as a sensitive tool to detect the presence of insecticides was assessed. The affinity partially purified fraction was utilized as a source of AChE for in vitro assay of the effect of insecticides on the AChE. The resultant IC50 values were then compared with published results.  An IC50 confidence interval comparison chart coined Shukor’s chart was utilized to compare the sensitivity of the AChE from H. nemurus to various other published results. In this chart, the IC50 confidence interval (95%) values for a particular insecticide is placed at one end of the chart and lined up with published values of IC50. Two lines are then made crossing the entire chart and represent the 95% confidence interval value for H. nemurus. Any confidence interval values for targeted insecticides from published results that do not touch these two lines are deemed significantly different (p<0.05), either more or less sensitive judging whether they fall below or above the two lines, respectively, whilst any confidence interval values that fall in or touch the two lines are deemed comparable in sensitivity.The results show that the AChE from H. nemurus is more sensitive to diazinonoxon and comparable in sensitivity compared to commercial and other local fish sources suggesting that the ACHE from H. nemurus can be a cheaper alternative for the current commercially and locally available AChEs. 

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Published

31.07.2017

How to Cite

Asri, N. A. M., Begum, G., Ahmad, S. A., Yasid, N. A., & Shukor, M. Y. (2017). A confidence interval comparison chart to assess the sensitivity of acetylcholinesterase from Hemibagrus nemurus as a source of enzyme for insecticides detection. Journal of Environmental Microbiology and Toxicology, 5(1), 14–20. https://doi.org/10.54987/jemat.v5i1.416

Issue

Vol. 5 No. 1 (2017)

Section

Articles

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