Emulsification Characteristics of Rhamnolipids by Pseudomonas aeruginosa Using Coconut Oil as Carbon Source

Authors

  • Izuani Mohamad Sobri Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Murni Halim Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Oi-Ming Lai Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Ahmad Firdaus Lajis Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Mohd Termizi Yusof Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Mohd Izuan Effendi Halmi Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Wan Lutfi Wan Johari Department of Environmental Sciences, Faculty of Environmental Studies, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Helmi Wasoh Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

DOI:

https://doi.org/10.54987/jemat.v6i1.400

Keywords:

Rhamnolipids, biosurfactants, Pseudomonas aeruginosa, rhamnose, coconut oil

Abstract

Rhamnolipids (RLs) production using coconut oil as a carbon source by the bacterium P. aeruginosa is studied. This bacterium was grown in media containing 1% carbon source (glucose/ coconut oil). The RLs were characterized by emulsification index (E24), thermal stability and oil spreading test. Further RLs quantification was carried out by the orcinol assay with L-rhamnose as the standard. The result showed that the highest production of RLs occurred in the presence of both coconut oil and glucose at 96 h (2.51 g/L). A stable emulsification index (E24) was observed using diesel with a maximum value of 57% at room temperature. Good stability to high temperature (120 oC) was observed when exposed at 55%. Oil displacement activity showed the presence of RLs with the highest value was at the highest RLs production. This study shows P. aeruginosa is able to produce RLs using coconut oil as the substrate and may potentially become a good source of biosurfactant for industry in the future.

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Published

31.07.2018

How to Cite

Sobri, I. M., Halim, M., Lai, O.-M., Lajis, A. F., Yusof, M. T., Halmi, M. I. E., Johari, W. L. W., & Wasoh, H. (2018). Emulsification Characteristics of Rhamnolipids by Pseudomonas aeruginosa Using Coconut Oil as Carbon Source. Journal of Environmental Microbiology and Toxicology, 6(1), 7–12. https://doi.org/10.54987/jemat.v6i1.400

Issue

Vol. 6 No. 1 (2018)

Section

Articles

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