Toxicity Assessment of Bioluminescent Rapid Bioassays (Vibrio fischeri) on Selected DBPs

Authors

  • Mohd Izuan Effendi Halmi Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
  • R.K.I. Phang Department of Environmental Sciences, Faculty of Environmental Studies, Universiti Putra Malaysia, 43400 UPM Serdang Selangor Malaysia
  • W.L.W. Johari Department of Environmental Sciences, Faculty of Environmental Studies, Universiti Putra Malaysia, 43400 UPM Serdang Selangor Malaysia
  • M.Y. Shukor Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia

DOI:

https://doi.org/10.54987/jemat.v2i2.169

Keywords:

iodoacetate, bioluminescence, disinfection, byproducts

Abstract

For the past 30 years more than 600 different disinfection by-products (DBPs) have been reported with many unknown ones yet to be discovered. Bioluminescence rapid toxicity tests are suitable toxicity screening for DBPs that caused adverse health effects. Previously, IC50 study on specific DBPs have not been conducted. This study aims to characteristically identify the sensitivity of bioluminescent rapid bioassays on selected DBPs (chloroacetic acid, trichloroacetic acid, bromoacetic acid and iodoacetic acid) by measuring IC50 of Vibrio fischeri on these compounds. IC50 are determined through luminescence that was measured using a Beckman Counter DTX 800 multimode detector.  The 30-minute IC50 of selected DBPs are as followed: CAA (865.4 mg/L), TCAA (1119 mg/L), BAA (59.67 mg/L) and IAA (15.6 mg/L). It was found that Bioluminescent Rapid Bioassays based on Vibrio fischeri showed promising sensitivity on the selected DBPs and are suitable as a screening tools for DBPs.

Author Biography

Mohd Izuan Effendi Halmi, Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia

Department of Biochemistry

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Published

30.12.2014

How to Cite

Halmi, M. I. E., Phang, R., Johari, W., & Shukor, M. (2014). Toxicity Assessment of Bioluminescent Rapid Bioassays (Vibrio fischeri) on Selected DBPs. Journal of Environmental Microbiology and Toxicology, 2(2), 47–52. https://doi.org/10.54987/jemat.v2i2.169

Issue

Vol. 2 No. 2 (2014)

Section

Articles

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