Toxicity and Bioaccumulation of Lead (Pb) in the Marine Bivalve Geloina expansa
DOI:
https://doi.org/10.54987/jemat.v13i2.1147Keywords:
Geloina expansa, Lead, Toxicity, LC50, BioaccumulationAbstract
Heavy metal pollution is a major environmental concern due to its persistence, toxicity, and ability to bioaccumulate in aquatic ecosystems. In Malaysia, increasing attention has been given to monitoring heavy metals in local marine organisms, as their accumulation in sediments and biota threatens ecosystem health and food safety. Bivalves serve as effective bioindicators, and LC50 provides essential insight into the acute toxicity of heavy metals across species. This study investigated the effects of lead (Pb) exposure on the bivalve Geloina expansa through a 96-hour acute toxicity assay. Mortality increased with rising Pb concentrations, accompanied by higher Pb accumulation in soft tissues. The 96-hour LC50 for G. expansa was calculated as 218.23 mg/L. Exposure to 250 mg/L Pb resulted in substantial Pb accumulation (1653 ± 177.51 mg/kg) compared to controls (13.00 ± 1.04 mg/kg), confirming effective uptake. These findings highlight Geloina expansa sensitivity to Pb and its suitability for pollution monitoring.
References
Shukor NA, Sughanti M, Krishnan K, Shukor NI, Wong LS. Assessing the ecological impact of heavy metal pollution in the sediments of Sepang Besar River, Malaysia. Nat Eng Sci 2024;9(3):117-28. http://doi.org/10.28978/nesciences.1558330
Strehse JS, Maser E. Marine bivalves as bioindicators for environmental pollutants with focus on dumped munitions in the sea: a review. Mar Environ Res 2020;158:105006. http://doi.org/10.1016/j.marenvres.2020.105006 (PubMed)
Yap CK, Tan WS, Ismail MS, Ariffin N, Cheng WH, Bintal A, Zakaly HM, Peng SH, Yap CW, Edward FB. Shells of intertidal mudflat snails: a promising biomonitoring material of nickel pollution. Environ Prot Res 2022:1–9.
Ong MC, Ibrahim A. Determination of selected metallic elements in marsh clam Polymesoda expansa collected from Tanjung Lumpur Mangrove Forest, Kuantan, Pahang. Borneo J Mar Sci Aquacult 2017;1. https://doi.org/10.51200/bjomsa.v1i0.993
Wariski I, Siregar YI, Amin B. Heavy metal content in sediment and Cerithidea obtusa in Panipahan waters, Rokan Hilir, Riau. J Natur Indones 2021;19(2):29–36. https://doi.org/10.31258/jnat.19.2.29-36
Krishnakumar PK, Qurban MA, Sasikumar G. Biomonitoring of trace metals in coastal waters using bivalve molluscs. Trace Elem Hum Health Environ 2018;153. https://doi.org/10.5772/intechopen.76938
Morling K, Fuchs S. Modelling copper emissions from antifouling paints applied on leisure boats into German water bodies. Environ Pollut 2021;289:117961. http://doi.org/10.1016/j.envpol.2021.117961 (journal DOI pattern)
Lim YC, Chen CF, Tsai ML, Wu CH, Lin YL, Wang MH, Albarico FP, Chen CW, Dong CD. Impacts of fishing vessels on heavy metal contamination in sediments: a case study of Qianzhen fishing port, southern Taiwan. Water 2022;14(7):1174. http://doi.org/10.3390/w14071174
Banik S, Das K, Islam M, Salimullah M. Recent advancements and challenges in microbial bioremediation of heavy metals contamination. JSM Biotechnol Biomed Eng 2014;2(1):1035.
Alluri HK, Ronda SR, Settalluri VS, Bondili JS, Suryanarayana V, Venkateshwar P. Biosorption: an eco-friendly alternative for heavy metal removal. Afr J Biotechnol 2007;6(25). https://doi.org/10.5897/ajb2007.000-2461
Yunus K, Suhaimi M, Zahir M, John A, Khan Chowdhury AJ, Saad S, Goddard JS. Bioaccumulation of some metals by Green mussel Perna viridis (Linnaeus 1758) from Pekan, Pahang, Malaysia. Int J Biol Chem 2011;5(1):54–60. https://doi.org/10.3923/ijbc.2011.54.60
Imsilp K, Tanhan P, Lansubsakul N, Thong-asa W. Oxidative response to cadmium and lead accumulation in Portunus pelagicus from Trang Province coast, Southern Thailand. J Aquat Anim Health 2024;36(3):265–74. https://doi.org/10.1002/aah.10229
Emami H, Abtahi B, Shokri MR. Heavy metal bioaccumulation (Ni, V, Hg) in soft tissues of crustaceans, bivalves and gastropods: a case study on the northern Persian Gulf. Casp J Environ Sci 2024;22(2):255–65.
Food M. Regulation (MFR). Malaysia law on food and drugs. Malaysia Law Publisher; 1985.
European Commission. Commission Regulation (EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs. Off J Eur Union 2006;L364:5–24.
Ekelund Ugge GM, Sahlin U, Jonsson A, Berglund O. Transcriptional responses as biomarkers of general toxicity: a systematic review and meta-analysis on metal-exposed bivalves. Environ Toxicol Chem 2023;42(3):628–41. http://doi.org/10.1002/etc.5482 (inferred DOI format likely valid)
Chuan OM, Kamaruzaman MI, Chuen YJ, Yunus KB, Bidai J. Metals contamination using Polymesoda expansa as bio-indicator in Kelantan River, Malaysia. Malays J Anal Sci 2017;21(3):597–604. https://doi.org/10.17576/mjas-2017-2103-09
Yahya N, Idris I, Rosli NS, Bachok Z. Mangrove-associated bivalves in Southeast Asia: a review. Reg Stud Mar Sci 2020;38:101382. http://doi.org/10.1016/j.rsma.2020.101382
Wakid SA, Abd Aziz NA, Alias Z, Mustafa M, Syazwan WM, Zulkifli SZ. Evaluation of glutathione S-transferase expression as biomarkers by heavy metals in Geloina expansa from Sepang Besar River, Selangor, Malaysia. Pertanika J Sci Technol 2024;32(4). https://doi.org/10.47836/pjst.32.4.01
Ramakritinan CM, Chandurvelan R, Kumaraguru A. Acute toxicity of Cu, Pb, Cd, Hg and Zn on marine molluscs Cerithedia cingulata and Modiolus philippinarum. Indian J Geo-Mar Sci 2012;41(2):141–5.
Sprague JB. The ABCs of pollutant bioassay using fish. In: Biological Methods for the Assessment of Water Quality, STP 528. ASTM International; 1983:6–30.
US Environmental Protection Agency. Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms. US EPA; 2002 Oct.
US Environmental Protection Agency. Method 3050B: SW-846 Test Methods for Evaluating Solid Wastes. 1996.
US Environmental Protection Agency. Method 6010B: Inductively Coupled Plasma-Atomic Emission Spectrometry. SW-846 Test Methods for Evaluating Solid Wastes. 1996.
Organisation for Economic Co-operation and Development. OECD Guideline for Testing of Chemicals No. 301: Ready Biodegradability. OECD; 1992. http://doi.org/10.1787/9789264070349-en
Tyler-Walters H, Williams E, Mardle MJ, Lloyd KA. Sensitivity assessment of contaminant pressures: approach development, application, and evidence reviews. Plymouth: Marine Life Information Network (MarLIN), Marine Biological Association of the UK; 2022. 192 p. Available from: https://www.marlin.ac.uk/publications
Labrot F, Narbonne JF, Ville P, Saint Denis M, Ribera D. Acute toxicity, toxicokinetics, and tissue target of lead and uranium in the clam Corbicula fluminea and the worm Eisenia fetida: comparison with the fish Brachydanio rerio. Arch Environ Contam Toxicol 1999;36(2):167–78. http://doi.org/10.1007/s002449900477
Hariharan G, Purvaja R, Ramesh R. Toxic effects of lead on biochemical and histological alterations in green mussel (Perna viridis) induced by environmentally relevant concentrations. J Toxicol Environ Health A 2014;77(5):246–60. http://doi.org/10.1080/15287394.2014.880064
Nadella SR, Tellis M, Diamond R, Smith S, Bianchini A, Wood CM. Toxicity of lead and zinc to developing mussel and sea urchin embryos: critical tissue residues and effects of dissolved organic matter and salinity. Comp Biochem Physiol C Toxicol Pharmacol 2013;158(2):72–83. http://doi.org/10.1016/j.cbpc.2013.07.006
Brahma N, Gupta A. Acute toxicity of lead in freshwater bivalves Lamellidens jenkinsianus obesa and Parreysia corrugata with evaluation of sublethal effects. Ecotoxicol Environ Saf 2020;189:109939. http://doi.org/10.1016/j.ecoenv.2019.109939
Hertika AM, Kusriani K, Indrayani E, Nurdiani R, Putra RB. Relationship between heavy metals (Pb, Cd, Hg) and metallothionein in gills and stomach of Crassostrea iredalei and Crassostrea glomerata. F1000Res 2018;7:1239. http://doi.org/10.12688/f1000research.15829.2
Ainar SG, Kurhe A. Acute toxicity of heavy metals (CdCl2 and HgCl2) on freshwater bivalve Lamellidens marginalis from Godavari River, India. World J Pharm Life Sci 2020;6(7):159–64.
Kumar S, Pandey RK, Das S, Das VK. Acute toxicity and behavioural responses of Lamellidens marginalis to dimethoate exposure. Recent Res Sci Technol 2013;4(11).
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Copyright (c) 2025 Sarini Ahmad Wakid, Nor Azwady Abd Aziz, Zazali Alias, Muskhazli Mustafa, Wan Mohd Syazwan, Syaizwan Zahmir Zulkifli
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