Characterization of Streptococcus agalactiae Strain TP540K and Its Potential as a Target for DNA Aptamer Development in the Preliminary Stage of Whole-Cell Based SELEX

Authors

  • ZiXuen Gan Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • Pei Qin Tan Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • Murni Halim Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • Nur Adeela Yasid Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • Mohd Termizi Yusof Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Amalia Mohd Hashim Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Jaafar Abdullah Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Nik Mohd Afizan Nik Abd Rahman Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Ina Salwany Md Yasin Aquatic Animal Health and Therapeutics Laboratory, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Firdaus Mohamad Hamzah Centre for Defence Foundation Studies, Universiti Pertahanan Nasional Malaysia, Kem Sungai Besi, 57000 Sungai Besi, Kuala Lumpur, Malaysia.
  • Mohd Yunus Shukor Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • Helmi Wasoh Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

DOI:

https://doi.org/10.54987/jemat.v12i2.1010

Keywords:

Streptococcus agalactiae, Streptococcus outbreak, DNA aptamer, Biochemical characteristics, Whole-cell based SELEX

Abstract

Streptococcus outbreak caused mainly by Streptococcus agalactiae leads to massive mortalities of freshwater aquatic organisms, especially cultured tilapias. This has caused a huge concern in the Malaysian aquaculture industry due to a lower aquaculture production affected by the outbreak. Thus, there is a need for a rapid and simple method to detect and monitor S. agalactiae in cultured fishponds. This study aims to investigate the characteristics of a local S. agalactiae strain TP540K and its potential as a target bacterium to develop DNA aptamer through the whole-cell based ‘systematic evolution of ligands by exponential enrichment (SELEX)’ procedure. The morphology and biochemical characteristics of S. agalactiae strain TP540K were studied. Furthermore, the preliminary stage of the SELEX process was carried out by incubating S. agalactiae strain TP540K with a single-stranded DNA library. In this study, S. agalactiae strain TP540K was confirmed to be spherical, gram-positive, catalase and oxidase-negative with beta-haemolytic characteristics. Additionally, the presence of bands with an estimated size of 80-90 base pairs on the agarose gel indicated the presence of DNA aptamer that could bind towards S. agalactiae strain TP540K during the SELEX process. The findings herein demonstrate that S. agalactiae strain TP540K has the potential to be utilised as a target for the production of DNA aptamers, and subsequent rounds of whole-cell based SELEX process can be performed to investigate the binding affinity and specificity for more accurate detection of S. agalactiae in a future application.

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Published

25.12.2024

How to Cite

Gan, Z., Tan, P. Q. ., Halim, M., Yasid, N. A., Yusof, M. T. ., Hashim, A. M. ., Abdullah, J. ., Rahman, N. M. A. N. A., Yasin, I. S. M. ., Hamzah, F. M. ., Shukor, M. Y., & Wasoh, H. (2024). Characterization of Streptococcus agalactiae Strain TP540K and Its Potential as a Target for DNA Aptamer Development in the Preliminary Stage of Whole-Cell Based SELEX. Journal of Environmental Microbiology and Toxicology, 12(2), 31–37. https://doi.org/10.54987/jemat.v12i2.1010

Issue

Vol. 12 No. 2 (2024)

Section

Articles

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Copyright (c) 2024 ZiXuen Gan, Pei Qin Tan, Murni Halim, Nur Adeela Yasid, Mohd Termizi Yusof, Amalia Mohd Hashim, Jaafar Abdullah, Nik Mohd Afizan Nik Abd Rahman, Ina Salwany Md Yasin, Firdaus Mohamad Hamzah, Mohd Yunus Shukor, Helmi Wasoh

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