Bioremediation of Petroleum-Impacted Soil Using Poultry Manure
DOI:
https://doi.org/10.54987/jemat.v12i2.1003Keywords:
Biostimulation, Hydrocarbon, Crude oil, Soil pollution, BiodegradationAbstract
This 60-day study investigated the use of poultry manure (PM) at 10% w/w to drive crude oil elimination in impacted soil at a loading contaminant concentration of 5% v/w. Gas chromatography determined removal efficiency while the bacterial community was analysed using standard microbiological techniques. The presence of crude oil as a contaminant made the soil more alkaline and reduced its nutrient content across the board. The addition of PM improved soil nutrient properties and brought soil pH closer to neutral. PM amendment resulted in total petroleum hydrocarbon elimination levels of 96.83%. The total heterotrophic bacterial (THB) and hydrocarbon utilising bacterial (HUB) abundance in the PM-treated soils showed a steady rise till day 40 with values of 7.93 Log CFU/g and 8.83 Log CFU/g respectively, after which mean counts dropped. Statistically significant differences at 95% confidence interval (p≤0.05) in TPH elimination were seen between the control studies and the PM-treated soil. THB and HUB counts also differed significantly (p≤0.05) between the unpolluted control and the PM treatment study but no statistically significant differences were obtained between the PM-treated soil and the polluted control. Bacterial diversity analysis revealed a predominance of Bacillus spp., Staphylococcus spp., and Micrococcus on day 0. Dominance shifted to Klebsiella pneumoniae, Bacillus, and Citrobacter spp. by the end of the study, indicating that these genera were the drivers of crude oil biodegradation during the study. The application of poultry manure effectively enhanced the biodegradation of crude oil in impacted soil.
References
Awewomom J, Dzebie F, Takyi YD, Ashie WB, Ettey EN, Afua PE, et al. Addressing global environmental pollution using environmental control techniques: A focus on environmental policy and preventive environmental management. Discov Environ. 2024;2:8.
Adeniran MA, Oladunjoye MA, Doro KO. Soil and groundwater contamination by crude oil spillage: A review and implications for remediation projects in Nigeria. Front Environ Sci. 2023;11:1137496.
ITOPF. Oil tanker spill statistics 2023 [Internet]. 2024 [cited 2024 Dec 22]. Available from: https://www.itopf.org/news-events/news/oil-tanker-spill-statistics-2023/.
National Oil Spill Detection and Response Agency (NOSDRA). Oil Spill Monitor [Internet]. NOSDRA; 2024 [cited 2024 Dec 22]. Available from: https://nosdra.oilspillmonitor.ng/.
Michael-Igolimah U, Abbey SJ, Ifelebuegu AO. A systemic review on the effectiveness of remediation methods for oil-contaminated soils. Environ Adv. 2022;9:100319.
Techtmann SM, Domingo JS, Conmy R, Barron M. Impacts of dispersants on microbial communities and ecological systems. Appl Microbiol Biotechnol. 2023;107(4):1095-1106.
Talvenmäki H, Saartama N, Haukka A, Lepikko K, Pajunen V, Punkari M. In situ bioremediation of Fenton's reaction-treated oil spill site with a soil inoculum, slow-release additives, and methyl-?-cyclodextrin. Environ Sci Pollut Res. 2021;28:20273-89.
Bala S, Garg D, Thirumalesh BV, Sharma M, Sridhar K, Inbaraj BS. Recent strategies for bioremediation of emerging pollutants: A review for a green and sustainable environment. Toxics. 2022;10:484.
Ho TTK, Tra VT, Le TH, Nguyen N, Tran C, Nguyen P, et al. Compost to improve sustainable soil cultivation and crop productivity. Case Stud Chem Environ Eng. 2022;6:100211.
Bremner JM. Nitrogen Total. In: Sparks DL, editor. Methods in Soil Analysis, Part 3, Chemical Methods. Madison, WI: Am Soc Agron; 1996.
Association of Official Analytical Chemists (AOAC). Official Methods of Analysis. 14th ed. Washington, DC: AOAC; 1990.
United States Environmental Protection Agency (USEPA). Method 3560 - Supercritical extraction for total recoverable petroleum hydrocarbons (TRPHs). Washington, DC: USEPA; 1996.
Cheesbrough M. Discrete Laboratory Practice in Tropical Countries. 2nd ed. Cambridge, UK: Cambridge University Press; 2005. p. 472-90.
Uwadiae SE, Obasi CD. Modelling of bioremediation of oil-contaminated soil using chicken droppings as biostimulant. J Appl Sci Environ Manage. 2021;25(11):1895-8.
Uwidia IE, Uwidia OE. Effects of time on pH, total bacteria counts, and total hydrocarbon content in the bioremediation of crude oil-contaminated soil using indigenous biostimulants. Tanzan J Sci. 2021;47(1):344-52.
Obasi NA, Eberechukwu E, Anyanwu DI, Okorie UC. Effects of organic manures on the physicochemical properties of crude oil-polluted soils. Afr J Biochem Res. 2013;7(6):67-75.
Li C, Cui C, Zhang J, Shen J, He B, Long Y, et al. Biodegradation of petroleum hydrocarbons-based pollutants in contaminated soil by exogenous effective microorganisms and indigenous microbiome. Ecotoxicol Environ Saf. 2023;253:114673.
Muhammad RG, Machido DA, Ado SA, Atta HI, Bello IA. A comparison of the effect of some organic wastes on the rate of bioremediation of soil contaminated with spent engine oil using gas chromatography/mass spectrometry. J Agric Agric Technol. 2019;19(1):36-52.
Osazee E, Adebola MO, Falusi OA, Abioye OP. Influence of organic waste on physicochemical properties of spent engine oil-contaminated soil supplemented with fungi isolated from mechanic workshops in Minna, Nigeria. Mediterr Publ Res Int J Med Biol Pharm Sci. 2019;9(9):77-87.
Hanson-Akpan RI, Obiekezie SO, Makut MD, Ekeleme KI, Muhammed IA. A comparative study on the bioremediation of spent engine oil-contaminated soil using cow dung, poultry manure, and sawdust. Int J Res Innov Appl Sci. 2023;8(10):145-58.
Naowasarn S, Leungprasert S. Abundance and diversity of hydrocarbon-utilizing bacteria in oil-contaminated soils throughout a remedial scheme using compost amendment. Songklanakarin J Sci Technol. 2019;41(1):12-20.
Koshlaf E, Shahsavari E, Haleyur N, Osborn AM, Ball AS. Effect of biostimulation on the distribution and composition of a polycyclic aromatic hydrocarbon-contaminated landfill soil during bioremediation. Geoderma. 2019;338:216-25.
Ezenne GI, Nwoke OA, Ezikpe DE, Obalum SE, Ugwushiwu BO. Use of poultry droppings for remediation of crude oil-polluted soils: Effects of application rate on total and polyaromatic hydrocarbon concentrations. Int Biodeterior Biodegrad. 2014;92:57-65.
Okafor UC, Orji MU, Agu KC, Awah NS, Okeke BC, Okafor OI, et al. Bioremediation of crude oil-polluted soil using broiler chicken droppings. J Appl Environ Microbiol. 2016;4(4):75-84.
Behera ID, Nayak M, Mishra A, Meikap BC, Sen R. Strategic implementation of integrated bioaugmentation and biostimulation for efficient mitigation of petroleum hydrocarbon pollutants from terrestrial and aquatic environments. Mar Pollut Bull. 2022;177:113492.
Ibrahim HMM. Biodegradation of used engine oil by novel strains of Ochrobactrum anthropi HM-1 and Citrobacter freundii HM-2 isolated from oil-contaminated soil. 3 Biotech. 2016;6:226.
Fagbemi OK, Sanusi AI. Chromosomal and plasmid-mediated degradation of crude oil by Bacillus coagulans, Citrobacter koseri, and Serratia ficaria isolated from soil. Afr J Biotechnol. 2017;16(21):1242-53.
Dankaka SM, Muhammed JB, Usman S, Jagaba AH, Abdullahi N. Phenol biodegradation by Acinetobacter baumannii and Citrobacter sedlakii isolated from petroleum product-contaminated environments. Case Stud Chem Environ Eng. 2023;8:100468.
Bahar S, Rehman A, Malik MS, Naz I, Jamil M, Anees M. Biodegradation of crude oil using bacterial strains isolated from oil-contaminated soil of Shakar Dara oil fields of Pakistan. Bioremediation J. 2024; Available from: https://doi.org/10.1080/10889868.2024.2428723.
Patowary K, Patowary R, Kalita MC, Deka S. Development of an efficient bacterial consortium for the potential remediation of hydrocarbons from contaminated sites. Front Microbiol. 2016;7:1092.
Tang F, Zhang H, Cheng H, Wang Y, Liu Q, Zhao C, et al. New insights of crude oil biodegradation construction by microbial consortium B10: Responded substrates, genomics, biodegradation mechanism, and pathways. Chem Eng J. 2023;478:147143.
Bertovi? B, Runjavec MS, Todorovic N, Zgrebec I, Domanovac MV. Biotechnological potential of oil-tolerant strains for possible use in bioremediation. Sustainability. 2024;16(2):563.
Abubakar A, Abioye OP, Aransiola SA, Maddela NR, Prasad R. Crude oil biodegradation potential of lipase produced by Bacillus subtilis and Pseudomonas aeruginosa isolated from hydrocarbon-contaminated soil. Environ Chem Ecotoxicol. 2024;6:26-32.
Hussain N, Muccee F, Hammad M, Mohiuddin F, Bunny SM, Shahab A. Molecular and metabolic characterisation of petroleum hydrocarbon-degrading Bacillus cereus. Pol J Microbiol. 2024;73(1):107-20.
Hussein MA, Hosee YN, Kadhim AI, Ibrahim UJ. The ability of Klebsiella pneumoniae and Klebsiella oxytoca to degrade oil waste. Nat Jurnal Pelaksanaan Pengabdian Bergerak bersama Masyarakat. 2024;2(3):1-7.
Karlapudi AP, Venkateswarulu TC, Tammineedi J, Kanumuri L, Ravuru BK, Dirisala VR, et al. Role of biosurfactants in bioremediation of oil pollution: A review. Petroleum. 2018;4(3):241-9.
Gomaa EZ, El-Meihy RM. Bacterial biosurfactant from Citrobacter freundii MG812314.1 as a bioremoval tool of heavy metals from wastewater. Bull Natl Res Cent. 2019;43:69.
Kaur H, Sangwam S, Sharma P, Singh S. Biosurfactant production prospects of a Gram-negative bacterium - Klebsiella pneumoniae spp. Ozaenae BK34. Environ Monit Assess. 2022;194:374.
Thirumurugan D, Kokila D, Balaji T, Rajamohan R, AlSalhi MS, Devanesan S, et al. Impact of biosurfactant produced by Bacillus spp. on biodegradation efficiency of crude oil and anthracene. Chemosphere. 2023;344:140340.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Anwuli U. Osadebe, Barisua Q. Nkoro
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
