Effects of Nigerian Walnut (Juglan nigra) Lectin on Haematological Parameters and Lethal Dose (LD50) on Swiss Wistar

Authors

  • Lawrence Alexander Department of Haematology. Federal Medical Centre, Lokoja, Nigeria.
  • Uchejeso Obeta Department of Medical Laboratory Management, Federal School of Medical Laboratory Science, Jos, Nigeria.
  • Michael Okungbowa Department of Haematology. College of Basic Medicine, University of Benin, Benin, Nigeria.
  • Isaac Emeje Department of Chemical Pathology, Federal Medical Centre, Lokoja, Nigeria.
  • Jonathan Sabulu Department of Obstetrics and Gynaecology, Federal Teaching Hospital Gombe, Nigeria.
  • Gumta Matthew Department of Family Medicine, OLA Hospital Jos, Nigeria
  • Priscilla Agbalaka Department of Parasitology, Federal School of Medical Laboratory Science, Jos, Nigeria.
  • Ezeanyagu Oluchi Department of Parasitology, Federal School of Medical Laboratory Science, Jos, Nigeria.

DOI:

https://doi.org/10.54987/jemat.v9i2.645

Keywords:

Juglan nigra, Nigerian walnut, Lectin, Haematological parameters, LD50

Abstract

This study was designed to investigate the effects of Juglan nigra lectin on haematological parameters and lethal dose of rats. An authenticated Juglan nigra (UBHP401) collected from Lokoja was used to extract lectin and treated on Swiss Wistar rats (n=35) and (n=17) with weights from 75-129g for haematological parameters and LD50 respectively. The Wistar rats were divided into five equal groups labeled A, B, C, D, and E. Lectin concentrations were prepared and administered at stipulated days. Group A served as control, group B received 30%, C received 50%, D received 70% and group E received 90% of lectin intraperitoneally. Samples (Baseline-BL, First Harvests-FH and Second Harvest-SH) were collected from all the groups and analyzed for the following haematological parameters: white blood count (WBC), Red Blood count (RBC), Haemoglobin (HB), packed cell volume (PCV), platelet count, neutrophil, lymphocyte, monocytes, eosinophil and basophil. The data were analysed using two-way analysis of variance (ANOVA) in graph pad prism version 5.03. The Eosinophil count and white blood cell count were significantly increased P<0.005 and 0.05, whereas the HB, PCV and RBC showed significant different P<0.05 based on the day of exposure of the animal to the lectin, the platelet count was significantly increased on the basis of days of exposure of P<0.05, Eosinophil significantly increased too. Juglan nigra, (Nigerian walnut) Lectin is nontoxic as noted from LD50 result. Nigerian walnut lectin has a positive effect in haematopoietic activity of Swiss Wistar rats.

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Published

2021-12-31

How to Cite

Alexander, L., Obeta, U., Okungbowa, M., Emeje, I., Sabulu, J., Matthew, G., Agbalaka, P., & Oluchi, E. (2021). Effects of Nigerian Walnut (Juglan nigra) Lectin on Haematological Parameters and Lethal Dose (LD50) on Swiss Wistar. Journal of Environmental Microbiology and Toxicology, 9(2), 33–37. https://doi.org/10.54987/jemat.v9i2.645

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