Inhibitory Effects of Chitosan-Silver Nanoparticles on Acetylcholinesterase from the Black Bean Aphid
DOI:
https://doi.org/10.54987/jemat.v14i1.212Keywords:
Chitosan, Fish scale waste, Silver nanoparticles, Acetylcholinesterase, Green synthesisAbstract
This study examined chitosan recovered from fish-scale waste, the synthesis of chitosan-silver nanoparticles (Ch-AgNPs), and their inhibition of acetylcholinesterase (AChE) from the black bean aphid, Aphis fabae. Fish scales were demineralized, deproteinized, and deacetylated under different solid-to-alkali ratios and temperatures. The resulting material and Ch-AgNP preparations were characterized by yield, pH, viscosity, water-binding capacity, fat-binding capacity, degree of deacetylation, and Fourier-transform infrared spectroscopy. The highest recorded chitosan yield (46.686%) occurred at a fish-scale-to-NaOH ratio of 1:2 and 55 °C. The degree of deacetylation ranged from 21.533% to 57.593%. Aphid AChE was purified by ammonium sulfate precipitation, DEAE-52 ion-exchange chromatography, and Superdex 200 gel filtration. The final preparation had a specific activity of 24.53 U mg−1, a reported purification fold of 229.40, and a recovery of 13.99%. Crude and purified AChE showed temperature optima near 30 °C; their pH optima were 7.0 and 7.5, respectively. Ch-AgNP preparations inhibited both enzyme preparations, although the IC50 pattern was not monotonic across the five formulations. These findings support further evaluation of fish-scale-derived Ch-AgNPs as enzyme-targeted materials, with additional nanoparticle characterization and bioassays needed before pesticidal efficacy can be established.
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