Entrapment of Mo-reducing Bacterium Increase its Resistance towards Mercury

Authors

  • Garba Uba Department of Science Laboratory Technology, College of Science and Technology, Jigawa State Polytechnic, Dutse, PMB 7040, Nigeria.
  • Mohd Yunus Shukor Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • Hafeez Muhammad Yakasai Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University Kano, Nigeria.

DOI:

https://doi.org/10.54987/jebat.v5i2.764

Keywords:

Bioremediation, Molybdenum, Molybdenum blue, Mercury, Dialysis tubing

Abstract

In ruminants, even trace amounts of molybdenum can be lethal. In areas with high pollution, molybdenum levels in soil and mine tailings can exceed 20,000 ppm. Bioremediation of molybdenum can be challenging when toxic mercury is also present. This research presents a novel approach using dialysis tubing and the molybdenum-reducing activity of Enterobacter sp. strain Dr. Y13 for molybdenum removal from aqueous solutions. Molybdenum blue (Mo-blue), produced during enzymatic reduction, is insoluble in dialysis tubing and this can be a twofold advantage as a method of removal and as a method to protect bacterial cells from heavy metal inhibition, especially mercury. In this experiment, we assess the toxicity-shielding effect of dialysis tubing for molybdenum reduction to Mo-blue by this bacterium in the presence of mercury. As the concentrations of mercury were increased, both free and immobilized cells were strongly inhibited. Modelling using the dissociationone phase exponential decay model gave an IC50 value for the immobilized form of 0.1107 mg/L (95% confidence interval from 0.073 to 0.217 while the IC50 value for the free cell system was 0.023 mg/L (95% C.I. from 0.019 to 0.028). Since the confidence interval for the IC50 values did not overlap, the immobilized system gave better protection from mercury than the free cell system. Toxicity to free cells was higher than toxicity to cells trapped in dialysis tubes, suggesting that trapping Mo-reducing cells may be an effective strategy for bioremediation of water or wastewater contaminated with multiple heavy metals.

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Published

2022-12-31

How to Cite

Uba, G., Shukor, M. Y., & Yakasai, H. M. (2022). Entrapment of Mo-reducing Bacterium Increase its Resistance towards Mercury. Journal of Environmental Bioremediation and Toxicology, 5(2), 36–39. https://doi.org/10.54987/jebat.v5i2.764

Issue

Vol. 5 No. 2 (2022)

Section

Articles

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