Determination of the Relationship Between C-Reactive Protein (CRP) Levels and Hepatitis B Surface Antigen

Authors

  • Maryam Buhari Gidado Deparment of Biotechnology, Faculty of Science, Nigerian Defence Academy, PMB 2109, Kaduna State, Nigeria.
  • Ali Ahmed Haroun Deparment of Biotechnology, Faculty of Science, Nigerian Defence Academy, PMB 2109, Kaduna State, Nigeria.
  • Yakubu Magaji Deparment of Biotechnology, Faculty of Science, Nigerian Defence Academy, PMB 2109, Kaduna State, Nigeria.
  • Zaharaddeen Abdullahi Deparment of Biotechnology, Faculty of Science, Nigerian Defence Academy, PMB 2109, Kaduna State, Nigeria.
  • Abdulsalami Muhammad Sani Deparment of Biotechnology, Faculty of Science, Nigerian Defence Academy, PMB 2109, Kaduna State, Nigeria.

DOI:

https://doi.org/10.54987/jebat.v5i2.761

Keywords:

C-reactive protein, Hepatitis B, Surface antigen, Elisa, Viral load

Abstract

The relationship between hepatitis B virus (HBV) infection and C-reactive protein (CRP), which is an inflammatory biomarker, is limited in studies on the general population. Thus, this study aimed at determining the relationship between CRP levels and Hepatitis B surface antigen in patients with hepatitis B. A total of 70 samples were screened for the presence of hepatitis surface antigen by one step hepatitis B Surface antigen test strip (serum/plasma) package insert. The samples were further subjected to ELISA test and quantitative real time PCR to determine the viral load. The performance of the assay on the 70 samples showed 17 (24.29%) patients were positive and 53 (75.71%) patients were negative for serological test. Out of the 17 samples which were positive for HBV, CRP was positive in 5 patients while 12 patients were negative for CRP. While out of 53 patients who were negative for HBV, 9 were positive for CRP and 44 were negative for CRP. For the significance of viral load for clinical monitoring, three titer groups were presented. Among the 70 samples tested for viral load of HBV, 50% (35/70) of samples showed low titer by the Ct˂30, while only 15.71% (11/70) of samples were detected with high viral load by Ct˃30. Statistical analysis showed insignificant relationship between CRP and HBV.  Positive predictive value of CRP was lower;it is revealed that the presence of HBV infection cannot be predicted on the basis of CRP analysis only. The reason behind lower CRP concentration in HBV positive cases remains unclear but there is a perception that high CRP levels in the blood can be a marker of inflammation.

References

Santos MB, Santos AD, da Silva PP, Barreto AS, dos Santos EO, Franca AVC, et al. Spatial analysis of viral hepatitis and schistosomiasis coinfection in an endemic area in Northeastern Brazil. Rev Soc Bras Med Trop. 2017;50(3):383-387.

Tuncer O, Selahattin A, Hasan A. Correlation of highly sensitive C-reactive protein and hepatitis C virus RNA in anti-HCV-positive sera. Dicle Med J. 2011;38:134-136.

Che W, Zhang B, Liu, W, Wei Y, Xu Y, Hu D. Association between high-sensitivity C-reactive protein and N-Terminal Pro-B-Type Natriuretic Peptide in patients with hepatitis C virus infection. Mediat Inflamm. 2012;730923.

Reingold J, Wanke C, Kotler D, Lewis C, Tracy R, Heymsfield S, et al. Association of HIV infection and HIV/HCV coinfection with C-reactive protein levels:The fat redistribution and metabolic change in HIV infection (FRAM) study. J Acquir. Immune Defic. Syndr. 2008;48:142-148.

Popovic L, Maja J, Miodrag V, Ljiljana K, Velimir K, Ivan D. High sensitivity C-reactive protein as prediction factor of disease progression in patients with chronic hepatitis C and mild liver steatosis. Acta Medica. 2010;49:14-18.

Shah S, Ma Y, Scherzer R, Huhn G, French AL, Plankey M, et al. Association of HIV, hepatitis C virus and liver fibrosis severity with interleukin-6 and C-reactive protein levels. AIDS. 2015;29(11):1325-1333.

Xie Y, Garza G, Dong J Hepatitis C virus genotype and subtype distribution in patient specimens tested at the University of Texas Medical Branch, Galveston. Lab Med. 2016;47:112-118.

Gale MJr, Foy EM. Evasion of intracellular host defence by hepatitis C virus. Nature. 2005;436:939-945.

Salter ML, Lau B, Mehta SH, Go VF, Leng S, Kirk GD. Correlates of elevated interleukin-6 and C-reactive protein in persons with or at high risk for HCV and HIV infections. J Acquir. Immune Defic. Syndr. 2013;64:488-495.

Ford DE, Erlinger TP. Depression and C-reactive protein in US adults:Data from the Third National Health and Nutrition Examination Survey. Arch Intern Med. 2004;164:1010-1014.

Goff DC, Lloyd-Jones DM, Bennett G, Coady S, D'Agostino RB, Gibbons R, et al. ACC/AHA Guideline on the assessment of cardiovascular risk. Circulation. 2014;129, S49-S73.

Del Giudice M, Gangestad SW. Rethinking IL-6 and CRP:Why they are more than inflammatory biomarkers, and why it matters. Brain Behav. Immun. 2018;70:61-75.

Perez M, Luyten K, Michel R, Riou C, Blondin B. Analysis of Saccharomyces cerevisiae hexose carrier expression during wine fermentation:both low and high affinity Hxt transporters are expressed. FEMS Yeast Res. 2005;5(4-5):351-61.

Odusanya O, Alufohai E, Meurice F, Ahonkhai V. Determinations of vaccination coverage in rural Nigeria. BMC Public Health. 2008;8(1):381.

Chernecky CC, Berger BJ Galactose-1-phosphate-blood. Laboratory Tests and Diagnostic Procedure. 6th ed. Philadelphia, PA:Elsevier. 2013;550.

Dietzen D. Amino Acids, Peptides and Proteins. Principles and Applications of Molecular Diagnostics. Washington University in St. Louis. 2018. Pp. 345-380.

Ridker PM, van't Klooster CC, Hjortnaes J, van der Graaf Y, Asselbergs FW, Westerink J, et al. The relation between systemic inflammation and incident cancer in patients with stable cardiovascular disease:a cohort study. Eur. Heart J 2019;40(48):3901-3909.

Laura J, Martin MD. Hepatocellular Carcinoma. Cancer Center, 2020;Portland, Oregon.

Chen S, Yang X, Feng, JF. A novel inflammation-based prognostic score for patients with esophageal squamous cell carcinoma:the c-reactive protein/prognostic nutritional index ratio. Oncotarget. 2016;7:62123-32.

Kogo M, Sunaga T, Nakamura S. Prognostic index for survival in patients with advanced non-small-cell lung cancer treated with third-generation agents. Chemotherapy. 2017;62:239-45.

Liu Y, Chen S, Zheng C. The prognostic value of the preoperative c-reactive protein/albumin ratio in ovarian cancer. BMC Cancer. 2017;17:285.

Paraskevis D, Beloukas A, Haida C, Katsoulidou A, Moschidis Z, Hatzitheodorou H, et al. Development of a new ultra-sensitive real-time PCR assay (ultra-sensitive RTQ-PCR) for the quantification of HBV-DNA. Virol. J. 2010;7:57-62.

Welzel TM, Miley WJ, Parks TL, Goedert JJ, Whitby D, Ortiz-Conde BA. Real-Time PCR Assay for Detection and Quantification of Hepatitis B Virus Genotypes A to G. J Clin Microbiol. 2006;44:3325-3333.

Berger A, Preiser W, Doerr HW. The role of viral load determination for the management of human immunodeficiency virus, hepatitis B virus and hepatitis C virus infection. J Clin Virol. 2001;20:23-30.

Pawlotsky JM. Hepatitis B virus (HBV) DNA assays (methods and practical use) and viral kinetics. Supplement. 2002;39(1):31-35.

Ganem D, Alfred MD, Prince M. Hepatitis B Virus Infection- Natural History and Clinical Consequences. N. Engl J Med. 2004;350:1118-1129.

Yim HJ, Lok AS. Natural history of chronic hepatitis B virus infection:what we knew in 1981 and what we know in 2005. Hepatology. 2006;43:S173-181.

Tan AT, Koh S, Goh V, Bertoletti A. Understanding the immune-pathogenesis of chronic hepatitis B virus:an Asian prospective. J Gastroenterol Hepatol. 2008;23:833-843.

Tanaka M, Katayama F, Kato H. Hepatitis B and C Virus Infection and Hepatocellular Carcinoma in China:A Review of Epidemiology and Control Measures. J Epidemiol. 2011;21:401-416.

Xu R, Zhang Z, Wang FS. Liver fibrosis:mechanisms of immune-mediated liver injury. Cell. Mol. Immunol. 2012;9:296-301.

Xiang WQ, Feng WF, Ke W. Hepatitis B virus X protein stimulates IL-6 expression in hepatocytes via a MyD88 dependent pathway. J Hepatol. 2011;54:26-33.

Kao JT, Lai HC, Tsai SM. Rather than interleukin-27, interleukin-6 expresses positive correlation with liver severity in naive hepatitis B infection patients. Liver Int. 2012;32:928-936.

Zhu C, Zhang R, Liu L. Hepatitis B virus enhances interleukin-27 expression both in vivo and in vitro. J Clin Immunol. 2009;131:92-7.

Xiang XG, Xie Q. IL-35:a potential therapeutic target for controlling hepatitis B virus infection. J Dig. Dis. 2012;16:1-6.

Hao S, Wang Y, Gao G, Li Z. Hepatitis B Virus Upregulates the Expression of C-reactive Protein Both in vivo and in vitro. Ann Clin Lab Sci. 2017;47(4):432-435.

Gedik M, Ozekinci T, Ozbek E, Yilmaz S, Atmaca S. Lack of correlation between CRP and Hepatitis B viral load in serum of patients with chronic HBV. J Infect. 2007;54:204.

Black S, Kushner I, Samols D. C-reactive protein. J Biol. Chem. 2004;279:48487-48490.

Agrawal A. CRP after 2004. Mol Immunol. 2005;42:927-930.

Bugdaci MS, Alkim C, Karaca C. Could complement C4 be an alternative to biopsy for chronic hepatitis B histopathologic findings?. J Clin Gastroenterol. 2011;45:449-455.

Whalley SA, Murray JM, Brown D, Webster GJ, Emery VC, Dusheiko GM, et al. Kinetics of acute hepatitis B virus infection in humans. J Exp Med. 2001;193:847-854.

Saldanha J, Gerlich W, Lelie N, Dawson P, Heermann K, Heath A. An international collaborative study to establish a World Health Organization international standard for hepatitis B virus DNA nucleic acid amplification techniques. Vox Sanguinis. 2001;80:63-71.

Doumbia M, Mbengue KA, Kakou ES, Sevede D, Dosso M. Molecular Characterization of Hepatitis B Virus isolated from patients in Côte d'Ivoire. J Microbiol Res Rev. 2013;5:61-66.

Kei F, Yasuhito T, Etsuro O, Tomoyoshi O, Takanobu K, Kanji S et al. Distribution of HBV genotypes among HBV carriers in Benin:Phylogenetic analysis and virological characteristics of HBV genotype E. World J Gastroenterol. 2005;41:6410-6415.

Jiwani NS, Gul RB. A silent storm:Hepatitis C in Pakistan. J Pak Med Stud. 2011;1:89-91.

Chen M, Huang C, Feng W, Li Y, Wu Y. C-reactive protein mediates the association between leisure-time physical activity and lung function in middle-aged and older adults. BMC Public Health. 2020;20(6):1471-2458.

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Published

2022-12-31

How to Cite

Gidado, M. B., Haroun, A. A., Magaji, Y., Abdullahi, Z., & Sani, A. M. (2022). Determination of the Relationship Between C-Reactive Protein (CRP) Levels and Hepatitis B Surface Antigen . Journal of Environmental Bioremediation and Toxicology, 5(2), 12–16. https://doi.org/10.54987/jebat.v5i2.761

Issue

Vol. 5 No. 2 (2022)

Section

Articles

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